CASP3 Antibody

Datasheet
Code CSB-PA786000
Size US$299
Uniprot No. P42574
Image
  • The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA786000(CASP3 (active) Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Jurkat cells, MCF7 cells, Primary antibody: CSB-PA786000(CASP3 (active) Antibody) at dilution 1/120, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
Immunogen Synthetic peptide of Human CASP3 (active)
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Tested Applications ELISA,WB,IHC;ELISA:1:1000-1:10000,WB:1:200-1:1000,IHC:1:25-1:100
Relevance This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Form Liquid
Conjugate Non-conjugated
Storage Buffer -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method Antigen affinity purification
Isotype IgG
Alias caspase 3, apoptosis-related cysteine peptidase
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Target Names CASP3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Tissue Specificity Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
Database Links

HGNC: 1504

OMIM: 600636

KEGG: hsa:836

STRING: 9606.ENSP00000311032

UniGene: Hs.141125

Pathway MAPK signaling pathway
p53 signaling pathway
TNF signaling pathway
Apoptosis
IL-17 signaling pathway
Natural killer cell mediated cytotoxicity
AGE-RAGE signaling pathway in diabetic complications
ER Stress pathway

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