MCC Antibody

Datasheet

Code	CSB-PA013571LA01HU
Size	US$166
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Image	IHC image of CSB-PA013571LA01HU diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA013571LA01HU diluted at 1:500 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA013571LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MCC Polyclonal antibody

Uniprot No.

P23508

Target Names

MCC

Alternative Names

Colorectal mutant cancer protein antibody; CRCM_HUMAN antibody; DKFZp762O1615 antibody; FLJ38893 antibody; FLJ46755 antibody; MCC 1 antibody; MCC antibody; MCC1 antibody; Mutated in colorectal cancers antibody; Protein MCC antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Colorectal mutant cancer protein (479-611AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MCC Antibody (Product code: CSB-PA013571LA01HU) is Non-conjugated. For MCC Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA013571LB01HU	MCC Antibody, HRP conjugated	ELISA
FITC	CSB-PA013571LC01HU	MCC Antibody, FITC conjugated
Biotin	CSB-PA013571LD01HU	MCC Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase (PAK) activation. Represses the beta-catenin pathway (canonical Wnt signaling pathway) in a CCAR2-dependent manner by sequestering CCAR2 to the cytoplasm, thereby impairing its ability to inhibit SIRT1 which is involved in the deacetylation and negative regulation of beta-catenin (CTNB1) transcriptional activity.

Gene References into Functions

Study described a novel tumor suppressor function of MCC in the regulation of E-cadherin mediated cell-cell adhesion in colorectal cancer cells. PMID: 29035389
Data suggest that millisecond dynamic changes in PDZ1 domain conformation are responsible for higher affinity of scribble PDZ1 for phosphorylated ligands; oligopeptide fragments of RPS6KA2 and MCC were used as ligands in these nuclear magnetic resonance chemical shift experiments. (RPS6KA2 = ribosomal protein S6 kinase 2; MCC = mutated in colorectal cancer protein) PMID: 29144123
MCC might confer alterative genetic susceptibility to colorectal cancer in individuals with schizophrenia promising to shed more light on the relationship between schizophrenia and cancer progression. PMID: 27226254
Our results indicate that in sharp contrast to its tumor suppressive role in colorectal cancer, MCC functions as an oncogene in B cells PMID: 25200342
cytoplasmic MCC-DBC1 interaction sequesters DBC1 away from the nucleus, thereby removing a brake on DBC1 nuclear targets, such as SIRT1 PMID: 24824780
targeted by miR-494, which is overexpressed in hepatocellular carcinoma PMID: 23913442
MCC regulates lamellipodia formation by binding to Scrib and its downstream partner Myosin-IIB in a multiprotein complex. PMID: 22480440
we have shown that promoter methylation of the APC gene does not extend to the neighbouring MCC gene in lung cancer, but LOH is found at both loci. PMID: 22542170
Promoter methylated MCC is associated with inflammatory bowel disease in colorectal cancer. PMID: 22213290
observed a significant association of the rs11283943 SNP with increased breast cancer risk in an Indian population PMID: 21279955
MCC is a nuclear, beta-catenin-interacting protein that can act as a potential tumor suppressor in the serrated colorectal cancer pathway by inhibiting Wnt/beta-catenin signal transduction. PMID: 18591935
Results identify MCC as a potential scaffold protein regulating cell movement and able to bind Scrib, beta-catenin and NHERF1/2. PMID: 19555689

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Subcellular Location

Cell membrane. Cell projection, lamellipodium. Nucleus. Cytoplasm. Note=Colocalizes with actin at the leading edge of polarized cells.

Protein Families

MCC family

Tissue Specificity

Expressed in a variety of tissues.

Database Links

HGNC: 6935

OMIM: 159350

KEGG: hsa:4163

STRING: 9606.ENSP00000386227

UniGene: Hs.593171

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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