TP53 Antibody

Datasheet
Code CSB-PA080174
Size US$167
Image
  • Immunohistochemical analysis of paraffin-embedded Mouse Colon Tissue using p53 Polyclonal Antibody.

  • Western blot analysis of 1) Mouse Brain Tissue, 2) Rat Brain Tissue using p53 Polyclonal Antibody.

Product Details

Uniprot No. P04637
Target Names TP53
Alternative Names Cellular tumor antigen p53
Raised in Rabbit
Species Reactivity Mouse,Rat
Immunogen Synthetic Peptide
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Concentration It differs from different batches. Please contact us to confirm it.
Buffer PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Form Liquid
Tested Applications WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:1000-2000
IHC 1:500-1000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Citations

Neuronal Ca2+ -dependent activator protein 1 (NCDAP1) induces neuronal cell death by activating p53 pathway following traumatic brain injury. Arai M, et al,Journal of Neurochemistry,2019

Applications: WB
Antibody dilution factor: 1: 1000
Review: (b and c) Western blot detection of NCDAP1, p53, Bax, and active caspase-3 in rat brain cortices or hippocampus at indicated time points after TBI. Box and whisker plots of protein intensity relative to loading control (b-actin) at the indicated time points are shown. Statistical analysis was performed by twoway ANOVA with Bonferroni post hoc test for (a) and one-way ANOVA with Dunnett post hoc test for (b, c). *p < 0.05, **p < 0.01, and ***p < 0.001 versus sham group; n = 4 of animals. CA1, cornu ammonis 1; DG, dentate gyrus; TUNEL, Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling; DAPI, 4',6-diamidino-2-phenylindole, dihydrochloride; NCDAP1, neuronal Ca2+2+-dependent activator protein 1; TBI, traumatic brain injury.
PMID: 31237956

Target Data

Function Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2
Involvement in disease Esophageal cancer (ESCR); Li-Fraumeni syndrome (LFS); Squamous cell carcinoma of the head and neck (HNSCC); Lung cancer (LNCR); Papilloma of choroid plexus (CPP); Adrenocortical carcinoma (ADCC); Basal cell carcinoma 7 (BCC7)
Subcellular Location Cytoplasm, Nucleus, Nucleus, PML body, Endoplasmic reticulum, Mitochondrion matrix, Note=Interaction with BANP promotes nuclear localization, Recruited into PML bodies together with CHEK2, Translocates to mitochondria upon oxidative stress, Translocates to mitochondria in response to mitomycin C treatment (PubMed:27323408), SUBCELLULAR LOCATION: Isoform 1: Nucleus, Cytoplasm, Note=Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4, SUBCELLULAR LOCATION: Isoform 2: Nucleus, Cytoplasm, Note=Localized mainly in the nucleus with minor staining in the cytoplasm, SUBCELLULAR LOCATION: Isoform 3: Nucleus, Cytoplasm, Note=Localized in the nucleus in most cells but found in the cytoplasm in some cells, SUBCELLULAR LOCATION: Isoform 4: Nucleus, Cytoplasm, Note=Predominantly nuclear but translocates to the cytoplasm following cell stress, SUBCELLULAR LOCATION: Isoform 7: Nucleus, Cytoplasm, Note=Localized mainly in the nucleus with minor staining in the cytoplasm, SUBCELLULAR LOCATION: Isoform 8: Nucleus, Cytoplasm
Protein Families P53 family
Tissue Specificity Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3
Database Links

HGNC: 11998

OMIM: 133239

KEGG: hsa:7157

STRING: 9606.ENSP00000269305

UniGene: Hs.437460

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