Recombinant Escherichia coli Protein RecA (recA)

1. These results suggest that an unpaired region acts as a kinetic trap for RecA-based nucleoprotein filaments, impeding the assembly mechanism. PMID: 28112216
2. Coupling of helicase and RecA-loading activity during dsDNA-end resection is crucial in avoiding the deleterious effects of a long and stabile 3' tail in E. coli. PMID: 28689072
3. RecA-based nucleoprotein filaments were shown to interact with dsDNA in a highly cooperative manner. PMID: 28977583
4. The regulation of RecA filament assembly and the mechanism by which RecA quickly and efficiently searches for and identifies a unique homologous sequence among a vast excess of heterologous DNA has been described. (Review) PMID: 27156117
5. Intracellular accumulation of d-serine increases RecA production. PMID: 27698085
6. models for how sequence imperfections may affect base triplet recognition by Rad51/RecA family members, and we discuss how these models and our results may relate to the different biological roles of RecA, Rad51, and Dmc1. PMID: 28476890
7. Rad51 and RecA juxtapose dsDNA ends ready for DNA ligase-catalyzed end-joining under recombinase-suppressive conditions. PMID: 27794044
8. These experiments suggest that ATP plays an unanticipated role in promoting the turnover of captured duplex DNA intermediates as RecA attempts to align homologous sequences during the early stages of recombination. PMID: 27587394
9. The results indicate that the toxicity of RecA D112R substitution is due to its persistent binding to duplex genomic DNA, creating barriers for other processes in DNA metabolism. PMID: 27124470
10. ATP binding is essential for the mutant version of RecA, RecA730, functions while ATP hydrolysis is required only for double-strand break repair. PMID: 27130282
11. Data indicate that large recombinase A (RecA)-independent genomic replacements are a novel recombinant class. PMID: 26162088
12. membrane acts as a scaffold for nucleating the formation of RecA filament bundles and plays an important role in the SOS response. PMID: 26481664
13. Regression of replication forks stalled by leading-strand template damage: regression by RecA is inhibited by SSB. PMID: 25138217
14. it is hypothesized that recA4162 suppresses SOS expression best when the ssDNA occurs at a gap and that uvrD303 is able to decrease SOS expression when the ssDNA is either at a gap or when it is generated at a DSB but does so better at a gap PMID: 24084169
15. Data shows recA providing tolerance through SOS response network responding to DNA damage caused by geraniol. PMID: 23906844
16. Given the opposite effects of RecA on Pol III and TLS replisomes, we propose that RecA acts as a switch to regulate the occupancy of polymerases within a moving replisome. PMID: 23509251
17. Using single-molecule microscopy, we directly visualize RecA filament assembly on single molecules of SSB-coated ssDNA, simultaneously measuring nucleation and growth. PMID: 23103864
18. Studies characterized the chromosomal replication and degradation patterns during thymine starvation in Rec+ cells, as well as in the recA and recBCD mutants. PMID: 22621921
19. The biochemical properties of the [S240N]RecA and [S240K]RecA proteins are described in this report. PMID: 22521886
20. MutL has little effect on RecA-ssDNA filament formation, but does down-regulate the ATPase activity of RecA. PMID: 22001225
21. These results reveal specific amino acid determinants of the RecA-LexA interaction and suggest that LexA binds RecA and RecAi+6 at a composite site on the RecA filament, which could explain the role of the active filament during LexA cleavage. PMID: 21912525
22. DNA strand exchange promoted by RecA is inhibited by M. tuberculosis HupB (MtHupB) even though M. tuberculosis HupB is not homologous with E. coli HU; RecA is unaffected by either N-terminal nor C-terminal domain of MtHupB alone. PMID: 21787377
23. These results emphasize the importance of the 5'-3' exonuclease for high constitutive SOS expression in recA730 mutants and show that RecBCD function can further enhance the excellent intrinsic abilities of the RecA730 protein in vivo. PMID: 21764927
24. Recombination potential of RecA is structurally suppressed. PMID: 21143322
25. new mechanism of the frequency of recombination exchanges increase by improving the synaptase activity of the RecA protein PMID: 20886744
26. Uropathogenic Escherichia coli strains defective in regulation of the SOS response mediated by RecA and LexA display attenuated virulence in immunocompetent mice within the first 6 h post infection. PMID: 20435157
27. Data show that recA4142, a novel recA(Con) mutant,is additionally dependent on ruvAB, recJ, xonA, and partially dependent on recQ for its high SOS(Con) expression. PMID: 20304994
28. RecA filament disassembly plays a major role in, and may be required for, DNA strand exchange PMID: 19910465
29. there is a C-terminal point mutation in RecA protein (E343K) that significantly alters the interaction between RecA and RecX proteins PMID: 15466870
30. recA mutants, being unable to repair fragmented chromosomes, depend on various strategies designed to avoid chromosomal fragmentation PMID: 15531636
31. Data show that UvrD, but not Rep, directly prevents homologous recombination in vivo, and that RecA contributes to toxicity in the rep uvrD mutant. PMID: 15565170
32. Nitrogen-containing amino acids are important in locking in the low-DNA affinity, more compact conformation of RecA PMID: 16008358
33. Two types of RecA-GFP foci have been defined in E.coli: 4,6-diamidino-2-phenylindole (DAPI)-sensitive foci that are bound to DNA and DAPI-insensitive foci that are DNA-less aggregates/storage structures. PMID: 16091045
34. These results suggested that RecA is a constitutive cellular factor that increases translocation of mini-TnMERI1 and may participate in dissemination of TnMERI1-like transposons. PMID: 16243449
35. RdgC inhibits RecA in Escherichia coli PMID: 16377615
36. Lys248 plays a significant role in ATP hydrolysis in trans across the subunit-subunit interface in the RecA nucleoprotein filament PMID: 16527806
37. RecA-catalyzed hydrolysis of a given nucleotide triphosphate or analogue thereof is exquisitely sensitive to certain structural elements of both the base and ribose moieties. Binding of nucleotides by RecA was found to be conformationally selective. PMID: 16584186
38. D100R RecA is characterized by inducible reduced activation of SOS response & diminished ability to promote cellular survival after UV irradiation. D100R substitution caused perturbation of RecA-ATP interactions & decrease in affinity of RecA for ssDNA. PMID: 16584187
39. Analysis of the single-stranded DNA sequence specificity of Escherichia coli RecA protein. PMID: 16684994
40. These findings indicate that the sequence specificity in recombination is achieved by Watson-Crick pairing in the context of base-pair dynamics inherent to the extended DNA structure bound by RecA during strand exchange. PMID: 16756994
41. The model is able to reproduce a wide range of observed helix pitches in transitions between compressed and stretched conformations of the RecA filament PMID: 16909421
42. trans-stimulation of pol V by RecA bound to ssDNA reflects a distinctive regulatory mechanism of mutation that resolves the paradox of RecA filaments assembled in cis on a damaged template strand obstructing translesion DNA synthesis PMID: 16929290
43. mechanisms of RecA-mediated three-strand homologous recombination PMID: 16946710
44. overall orientation and also the internal structure of RecA in the active filament are not markedly altered when the bound DNA changes from single- to double-stranded PMID: 16964978
45. direct observation of filament assembly on individual double-stranded DNA molecules using fluorescently modified RecA PMID: 16988658
46. These results support the proposal that the RecA protein restructures DNA, preparing it for the recognition of a complementary second DNA strand, and that the recognition is due mainly to direct base-base contacts between DNA strands. PMID: 17097680
47. These data show that the loading of RecA protein by RecBCD is necessary in vivo, and they show that RecA proteins with enhanced single-stranded DNA-binding capacity can partially bypass the need for RecBCD-mediated loading. PMID: 17141804
48. Study showed that the RecA-DNA filament disassembled in the direction from 5' to 3' of ssDNA as dATP hydrolysis proceeded. PMID: 17202195
49. in vivo role for UvrD in removing RecA from the DNA PMID: 17259317
50. RecA is the first reported cellular factor specifically affecting swarming but not swimming motility in E. coli. PMID: 17391508

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