Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

CD38

Uniprot No.

P28907

Research Area

Cancer

Alternative Names

CD38; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADPRC 1; Cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; T10; CD antigen CD38

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

43-300aa

Target Protein Sequence

VPRWRQQWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCNITEEDYQPLMKLGTQTVPCNKILLWSRIKDLAHQFTQVQRDMFTLEDTLLGYLADDLTWCGEFNTSKINYQSCPDWRKDCSNNPVSVFWKTVSRRFAEAACDVVHVMLNGSRSKIFDKNSTFGSVEVHNLQPEKVQTLEAWVIHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKFLQCVKNPEDSSCTSEI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

57.4 kDa

Protein Length

Extracellular Domain

Tag Info

C-terminal hFc1-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombinant human ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (CD38) production in mammalian cells involves co-cloning the gene encoding the extracellular domain of the human CD38 protein (43-300aa) into an expression vector with a C-terminal hFc-tag gene, followed by transformation into mammalian cells. The cells are cultured under conditions that induce protein expression. After sufficient growth, the cells are lysed to release the recombinant protein. Purification is achieved using affinity chromatography technique. The purity of the CD38 protein is confirmed using SDS-PAGE, reaching over 85%.

Human CD38 protein is a transmembrane glycoprotein that stimulates B-lymphocyte activation and acts as an enzyme that catalyzes the synthesis of cyclic ADP-ribose from NAD+ [1]. It is a part of the ADP ribosyl cyclase/CD38 gene family and is evolutionarily conserved, indicating its importance in human physiology [2]. CD38 functions as an ectoenzyme and a receptor, degrading NAD and modulating cellular NAD homeostasis [3]. Furthermore, CD38 is involved in oxytocin secretion, social behavior, and calcium regulation [4][5]. It is expressed in inflammatory conditions and is robustly induced in human macrophages under inflammatory stimuli [6]. CD38 is also implicated in diseases like cancer, where it is methylated in prostate cancer and regulates extracellular NAD+ [7].

References:
[1] M. Dong, Y. Qi, S. Sun, X. Pu, Z. Yang, L. Zhanget al., Design, synthesis and biological characterization of novel inhibitors of cd38, Organic & Biomolecular Chemistry, vol. 9, no. 9, p. 3246, 2011. https://doi.org/10.1039/c0ob00768d
[2] F. Malavasi, S. Deaglio, A. Funaro, E. Ferrero, A. Horenstein, E. Ortolanet al., Evolution and function of the adp ribosyl cyclase/cd38 gene family in physiology and pathology, Physiological Reviews, vol. 88, no. 3, p. 841-886, 2008. https://doi.org/10.1152/physrev.00035.2007
[3] E. Chini, C. Chini, J. Netto, G. Oliveira, & W. Schooten, The pharmacology of cd38/nadase: an emerging target in cancer and diseases of aging, Trends in Pharmacological Sciences, vol. 39, no. 4, p. 424-436, 2018. https://doi.org/10.1016/j.tips.2018.02.001
[4] H. Higashida, S. Yokoyama, M. Kikuchi, & T. Munesue, Cd38 and its role in oxytocin secretion and social behavior, Hormones and Behavior, vol. 61, no. 3, p. 351-358, 2012. https://doi.org/10.1016/j.yhbeh.2011.12.011
[5] V. Sathish, M. Thompson, S. Sinha, G. Sieck, Y. Prakash, & C. Pabelick, Inflammation, caveolae and cd38-mediated calcium regulation in human airway smooth muscle, Biochimica Et Biophysica Acta (Bba) - Molecular Cell Research, vol. 1843, no. 2, p. 346-351, 2014. https://doi.org/10.1016/j.bbamcr.2013.11.011
[6] S. Amici, M. Young, J. Narvaez-Miranda, K. Jablonski, J. Arcos, L. Rosaset al., Cd38 is robustly induced in human macrophages and monocytes in inflammatory conditions, Frontiers in Immunology, vol. 9, 2018. https://doi.org/10.3389/fimmu.2018.01593
[7] J. Mottahedeh, M. Haffner, T. Grogan, T. Hashimoto, P. Crowell, H. Beltranet al., Cd38 is methylated in prostate cancer and regulates extracellular nad+, Cancer & Metabolism, vol. 6, no. 1, 2018. https://doi.org/10.1186/s40170-018-0186-3

Target Background

Function

Synthesizes the second messengers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system.

Gene References into Functions

CD38 and cADPR are key players in the orches- tration of cellular responses to respiratory syncytial virus by infected monocyte-derived dendritic cells. PMID: 29178427
our data are consistent with the conclusion that CD38 plays a role in murine and human lung tumorigenesis PMID: 29228209
isatuximab decreases multiple myeloma cell- and bone marrow stromal cell-induced iTreg by inhibiting both cell-cell contact and TGFbeta/IL10. Finally, CD38 levels correlate with differential inhibition by isatuximab of Tregs from multiple myeloma versus normal donors.Targeting CD38 by isatuximab can preferentially block immunosuppressive Tregs and thereby restore immune effector function against multiple myeloma PMID: 28249894
Empathic response mediates the CD38-altruism association. PMID: 28865941
Increased levels of HLADR and CD38 expressions in peripheral blood were associated with oral lesions in HIV-positive patients. Periodontal disease was associated with HLADR expression. PMID: 28500735
Panobinostat increased CD38 expression in a dose-dependent manner in primary myeloma cells. This was specific for myeloma cells and did not occur in lymphoma cell lines. This increase enabled the antimyeloma activity of daratumumab. PMID: 28476749
To demonstrate the therapeutic effect of daratumumab in CLL, we generated a disseminated CLL mouse model with the CD38(+) MEC2 cell line and CLL patient-derived xenografts (CLL-PDX). Daratumumab significantly prolonged overall survival of MEC2 mice, completely eliminated cells from the infiltrated organs, and significantly reduced disease burden in the spleen of CLL-PDX PMID: 27637890
CD38 mRNA levels were correlated with lower Autism Quotient (AQ), indicating enhanced social skills. CD38 expression and CD157 eQTL SNPs altogether account for a substantial 14% of the variance in sociality. the ecological validity of these findings was demonstrated with subjects with higher PBL CD38 expression having more friends, especially for males. PMID: 28212520
CD38(lo) luminal cells are enriched in glands adjacent to inflammatory cells and exhibit epithelial nuclear factor kappaB (NF-kappaB) signaling. In response to oncogenic transformation, CD38(lo) luminal cells can initiate human prostate cancer in an in vivo tissue-regeneration assay PMID: 27926864
Results provide evidence that CD38 enhanced the proliferation and inhibited the apoptosis of cervical cancer cells by affecting the mitochondria functions. PMID: 28544069
these data demonstrate an important role for CD38 and complement-inhibitory protein expression levels in daratumumab sensitivity. PMID: 27307294
Results may imply that CD38 expression either reflects or participates in pathogenic mechanisms of HIV disease independently of cell cycling. PMID: 27064238
The data suggest that ZO-1, along with CD38 and Zap-70, plays a role in cell cycle regulation in chronic B cell leukemia, and may be used as a prognostic marker in the disease monitoring. PMID: 26306999
Primary human melanoma cell lines suppress in vitro T cell proliferation through an adenosinergic pathway in which CD38 and CD73 play a prominent role. PMID: 26329660
CD38 and its related genes are highly expressed in human nasopharyngeal carcinoma cell lines. PMID: 25630761
soluble CD38 (sCD38) in seminal plasma increases the capacitation of sperm via specific interactions between sCD38 and the CD31 on the sperm. PMID: 26407101
the expression of CD38+ on both CD4+, CD8+T lymphocytes from peripheral blood and CSF discriminated between viremic and non-viremic patients PMID: 26365593
study points to an association between maternal SNPs in the CD38 in Japanese women and susceptibility to preterm birth. PMID: 26025338
Peripheral blood CD38 bright CD8+ effector memory T cells predict acute graft-versus-host disease. PMID: 25881755
CD38 is expressed on human MDSC-like cell population that is expanded in the peripheral blood of advanced-stage cancer patients. PMID: 26294209
Upregulation of CD38 expression on multiple myeloma cells by all-trans retinoic acid improves the efficacy of daratumumab. PMID: 25975191
Genetic variation in CD38 and breastfeeding experience interact to impact infants' attention to social eye cues. PMID: 26371313
genetic polymorphism is associated with diffuse large B-cell lymphoma susceptibility in Egyptians PMID: 25564959
Hairy-cell leukemia patients that were CD38-positive had a shorter mean time to salvage therapy than CD38-negative ones. CD38 expression in HCL drives poor prognosis by promoting survival and heterotypic adhesion. PMID: 26170397
alterations in content of soluble molecules CD38 are associated with characteristics of tumor process that indicates at their monitoring significance under malignant neoplasms of uterus PMID: 26470437
The results indicate that the net charge of the N-terminal segment is important in determining the membrane topology of CD38 and that the type III orientation can be a functional form of CD38 for Ca(2)-signaling. PMID: 25447548
The majority of extranodal NK/T cell lymphoma cases were CD38 positive, which significantly correlated with poor outcomes. PMID: 25865943
High levels of CD38 reduced intracellular (NAD+) levels and blocked acquired resistance by inhibiting the activity of the NAD+-dependent SIRT1 deacetylase PMID: 24967705
CD38 status was associated with behavior and psychological reactions within live interactions, as well as global relationship quality PMID: 24396004
These results validate CD38 as a therapeutic target and support the current evaluation of this unique CD38-targeting functional antibody in phase I clinical trials in patients with CD38+ B-cell malignancies. PMID: 24987056
At the same time, CD38 overexpression affected the expression of PI3K, Akt, MDM2 and p53 in vivo PMID: 25310288
CD38 expression is regulated by micro-RNA 708 in airway smooth muscle cells. PMID: 25175907
CD38 has a role in chronic lymphocytic leukemia growth and trafficking PMID: 24990614
Chronic lymphocytic leukemia cells express CD38 in response to Th1 cell-derived IFN-gamma by a T-bet-dependent mechanism. PMID: 25505279
Increased numbers of circulating ICOS(+) and IL-21(+) Tfh and CD38(+) plasma cells may be exhibited by patients with recent diagnoses of primary biliary cirrhosis. PMID: 25404409
nominal associations were found between autism spectrum disorder scores and single-nucleotide polymorphisms in OXT, ARNT2 and CD38 PMID: 24635660
Autism and features of regression-previously acquired speech lost in the second year of life. The younger sister, who also had asthma, inherited a maternal deletion of 4p15.32 that results in a BST1-CD38 fusion transcript. PMID: 24634087
results demonstrate that CD38(+) cells are a useful model to study effects of the cellular NAD levels on cellular processes and establish a new linker between cellular NAD levels and oxidative stress PMID: 24295520
only one SNP in CD38 was significantly associated with social integration and that SNP predicted when using a dichotomized indicator of social connectedness, but not a continuous measure of social connectedness or the continuously married outcome. PMID: 24209975
Data suggest that the uniquely increased expression of CD38 and E2F2 in rheumatoid arthritis (RA) synovial tissues contribute to the immunoactivation of the disease. PMID: 24397353
PBMC from MM patients display a deregulated response related to CD38 activation pathway defects; CD38 may be functionally involved in the progression of this pathology via the secretion of high levels of IL-6 that protects neoplastic cells from apoptosis PMID: 24489445
These data suggest that both functional roles of CD38 might be important in the pathogenesis of B-CLL. PMID: 24216102
Atorvastatin and rosiglitazone did not affect the expression of CD38. PMID: 23686733
Data suggest CD38 (CD38 antigen (p45) protein) and CD49d (alpha4 Integrin; very late antigen-4 alpha) are more than just markers of an aggressive chronic lymphocytic leukemia (CLL) cell type and play functional roles in pathobiology of CLL. [REVIEW] PMID: 24288111
CD38 is expressed within caveolae and its function is linked to the caveolar regulatory proteins. PMID: 24275509
Report diagnosis of multiple myeloma using two-color flow cytometry based on kappa/lambda ratios of CD38-gated plasma cells. PMID: 23755763
CD38-cADPR mediates bile acid-induced pancreatitis and acinar cell injury through aberrant intracellular Ca(2+) signaling. PMID: 23940051
The frequency of CD38high antibody-secreting cells (ASCs) is increased during the acute phase of hepatitis A virus (HAV) infection. Substantial numbers of ASCs are non-HAV-specific and dominantly secrete IgM. PMID: 23729443
Data show that ADP-ribosylation of CtBP1-S/BARS by brefeldin A (BFA) occurs via synthesis of a BFA-ADP-ribose conjugate by the ADP-ribosyl cyclase CD38 and covalent binding of the BFA-ADP-ribose conjugate into the CtBP1-S/BARS NAD(+)-binding pocket. PMID: 23716697
CD38 signals upregulate expression and functions of matrix metalloproteinase-9 in chronic lymphocytic leukemia cells. PMID: 22955446

Hide All

Subcellular Location

Membrane; Single-pass type II membrane protein.

Protein Families

ADP-ribosyl cyclase family

Tissue Specificity

Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma.

Database Links

HGNC: 1667

OMIM: 107270

KEGG: hsa:952

STRING: 9606.ENSP00000226279

UniGene: Hs.479214

Code	CSB-MP004929HU1
Abbreviation	Recombinant Human CD38 protein, partial
MSDS	MSDS Datasheet
Size	$290
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (CD38), partial

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage