Recombinant Mouse Adenylate kinase isoenzyme 1 (Ak1)

Code CSB-YP870812MO
MSDS
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Source Yeast
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Code CSB-EP870812MO
MSDS
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Source E.coli
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Code CSB-EP870812MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP870812MO
MSDS
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Source Baculovirus
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Code CSB-MP870812MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ak1
Uniprot No.
Alternative Names
Ak1; Adenylate kinase isoenzyme 1; AK 1; EC 2.7.4.3; EC 2.7.4.6; ATP-AMP transphosphorylase 1; ATP:AMP phosphotransferase; Adenylate monophosphate kinase; Myokinase
Species
Mus musculus (Mouse)
Expression Region
1-194
Target Protein Sequence
MEEKLKKAKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRAEVSSG SERGKKLSAI MEKGELVPLD TVLDMLRDAM LAKVDSSNGF LIDGYPREVK QGEEFEQKIG QPTLLLYVDA GAETMTQRLL KRGETSGRVD DNEETIKKRL ETYYNATEPV ISFYDKRGIV RKVNAEGTVD TVFSEVCTYL DSLK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also possesses broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. May provide a mechanism to buffer the adenylate energy charge for sperm motility.
Gene References into Functions
  1. In addition to AK1 and AK2, which we previously demonstrated are present in outer dense fibers and mitochondrial sheath of the mouse sperm tail, we show that another AK, AK8, is present in a third flagellar compartment, the axoneme. PMID: 24740601
  2. CaMKIalpha regulates AMP kinase-dependent, TORC-1-independent autophagy during lipopolysaccharide-induced acute lung neutrophilic inflammation. PMID: 23447692
  3. identified hitherto unrecognized soluble forms of AK1 and NTPDase1/CD39 that contribute in the active cycling between the principal platelet-recruiting agent ADP and other circulating nucleotides PMID: 22637533
  4. AK1 is not involved in thiamine triphosphate synthesis in vivo PMID: 12379473
  5. Deficiency of this enzyme induces molecular and structural adaptations to support muscle energy metabolism. PMID: 12562761
  6. one of the consequences of M-CK and AK1 deficiency is hampered phosphoryl delivery to the actomyosin ATPase, resulting in a loss of contractile performance. PMID: 12730234
  7. Skeletal muscle of mdx mice has reduced expression and enzymatic activity of adenylate kinase 1, but increased enzymatic activity of creatine kinase PMID: 14625851
  8. The purpose of this study was to determine whether adenylate kinase 1 deficiency would result in sufficient ADP accumulation to be visible using (31)P-NMRS during the high energy demands of frequent in situ tetanic contractions. PMID: 15689408
  9. AK1 in the flagellar accessory structures provides a mechanism to buffer the adenylate energy charge for sperm motility PMID: 16790685
  10. Defective metabolic signaling in Ak1 gene knock-out hearts compromises post-ischemic coronary reflow. PMID: 17704060
  11. Adenylate kinase-1 knockout mice have a normal capacity for contraction-mediated glucose uptake. PMID: 17973952
  12. Creatine kinase (CK) and adenylate kinase (AK) are especially important in facilitating energy metabolism during very high energy demands. PMID: 19156695

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Subcellular Location
Cytoplasm.
Protein Families
Adenylate kinase family, AK1 subfamily
Database Links
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