Recombinant Rat GTPase HRas(Hras)

Code CSB-RP155374r
Size US$2466
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Hras
Uniprot No. P20171
Research Area Others
Alternative Names Hras; Hras1; GTPase HRas; H-Ras-1; Transforming protein p21; c-H-ras; p21ras) [Cleaved into: GTPase HRas; N-terminally processed]
Species Rattus norvegicus (Rat)
Source E.coli
Expression Region 2-186aa
Target Protein Sequence TEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKC
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 24.9kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Gene References into Functions
  1. Together, these results suggest a novel mechanism regulating PATZ1 expression based on the upregulation of miR-29b expression induced by Ras oncogene. PMID: 27125250
  2. H-ras isoform mediates protection against pressure overload-induced cardiac dysfunction in part through activation of AKT/PI3K signaling pathway. PMID: 28193718
  3. Phenotypic Screening Identifies Protein Synthesis Inhibitors as H-Ras-Nanocluster-Increasing Tumor Growth Inducers. PMID: 26568031
  4. H-Ras mediates the inhibitory effect of epidermal growth factor on the epithelial Na+ channel PMID: 25774517
  5. Data indicate that numerous proteins that are up-regulated in H-ras(G12V) transgenic rat pancreatic ductal adenocarcinoma (PDAC) are also up-regulated in human PDAC; therefore, this rat model can be used for diagnostic biomarkers for this disease. PMID: 23648844
  6. Fe(2+)-generated O(2) mediates p21(ras) and TAK1 activation via PTP inhibition and Lys(63)-polyUb of TRAF6 in caveosomes for proinflammatory M1 activation in hepatic macrophages PMID: 22829592
  7. The proto-oncogenic H-Ras is directly involved in the promotion of muscle differentiation via PI3K and its downstream signaling pathways. PMID: 20603646
  8. Data show that Myc repressed Ras-induced senescence, and that Cdk2 interacted with Myc at promoters, where it affected Myc-dependent regulation of genes, including those of proteins known to control senescence. PMID: 19966300
  9. We examined if H-Ras and K-Ras proteins, which are distributed across different plasma membrane microdomains, have equal access to the endocytic compartment and whether this access is necessary for downstream signaling. PMID: 12077341
  10. A positive regulatory role of Ras is observed in AMPA receptor-mediated synaptic transmission in hippocampal pyramidal neurons. PMID: 12824760
  11. Functional activation of H-Ras might be one of the signaling steps involved in glucose-induced capillary cell apoptosis. PMID: 14988264
  12. Silencing of individual genes by DNA methylation is controlled by HRAS oncogenic signalling pathways, yet the mechanisms responsible for initial target gene suppression are variable. PMID: 16568090
  13. H-ras suppresses integrin affinity via independent Raf and phospholipase C epsilon signaling pathways. PMID: 16895916
  14. analysis of tumor induction with expression plasmids for activated H-ras and c-myc PMID: 18218323
  15. Infection with M. arginini made rat and mouse embryo fibroblasts susceptible to transformation with oncogenic H-Ras PMID: 18408766
  16. induction of long-term potentiation triggered robust Ca2+-dependent Ras activation in single spines that decayed in approximately 5 minutes; Ras activity spread over approximately 10 micrometers of dendrite and invaded neighboring spines by diffusion PMID: 18556515
  17. trend of increased H-ras expression was observed in diabetic compared to normal rats during oncogenesis PMID: 19283661

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Subcellular Location Cell membrane, Cell membrane, Lipid-anchor, Cytoplasmic side, Golgi apparatus, Golgi apparatus membrane, Lipid-anchor
Protein Families Small GTPase superfamily, Ras family
Database Links

KEGG: rno:293621

STRING: 10116.ENSRNOP00000022363

UniGene: Rn.102180

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