Code | CSB-RA024077A392phHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
To create the phospho-TP53 (S392) recombinant monoclonal antibody, the process initiates with the isolation of genes responsible for coding this antibody from rabbits that have been previously exposed to a synthesized peptide originating from the human TP53 protein phosphorylated at S392. These antibody genes are then meticulously integrated into specialized expression vectors. Following this genetic modification, the vectors are thoughtfully introduced into host suspension cells, which are diligently cultivated to encourage the production and secretion of antibodies. After this cultivation phase, the phospho-TP53 (S392) recombinant monoclonal antibody undergoes a rigorous purification process using affinity chromatography techniques, effectively separating the antibody from the surrounding cell culture supernatant. Finally, the functionality of the antibody is comprehensively assessed through a battery of tests, including ELISA and WB, conclusively confirming its capability to interact effectively with the human TP53 protein phosphorylated at S392.
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