TP53 Antibody

Datasheet
Code CSB-PA07889A0Rb
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Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. P04637
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image
  • Western blot
    All lanes: Cellular tumor antigen p53 antibody at 2ug/ml
    Lane 1: A431 whole cell lysate
    Lane 2: 293T whole cell lysate
    Lane 3: MCF-7 whole cell lysate
    Lane 4: MM231 whole cell lysate
    Secondary
    Goat polyclonal to rabbit at 1/10000 dilution
    Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa
    Observed band size: 44 kDa

  • Western Blot
    Positive WB detected in: Mouse kidney
    All lanes: p53 antibody at 4ug/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa
    Observed band size: 44 kDa

  • Western Blot
    Positive WB detected in: HEK293 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, Mouse kidney tissue
    All lanes: p53 antibody at 2.7ug/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa
    Observed band size: 44 kDa

  • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA07889A0Rb at dilution of 1:100

  • Chromatin Immunoprecipitation Hela(1.2*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-p53 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP07889HU) against the P21 promoter.

  • Western Blot
    Positive WB detected in: Mouse brain tissue
    All lanes:TP53 antibody at 2.5ug/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 44, 38, 39, 40, 34, 35, 30, 24, 25 kDa
    Observed band size: 44 kDa

  • Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA07889A0Rb at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA07889A0Rb at dilution of 1:100

Immunogen Recombinant human Cellular tumor antigen p53 protein (2-393AA)
Raised in Rabbit
Species Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC, ChIP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200
Relevance Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediatedapoptosis.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Cellular tumor antigen p53 (Antigen NY-CO-13) (Phosphoprotein p53) (Tumor suppressor p53), TP53, P53
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area Cell Biology
Target Names TP53
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Function Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2
Involvement in disease Note=TP53 is found in increased amounts in a wide variety of transformed cells. TP53 is frequently mutated or inactivated in about 60% of cancers. TP53 defects are found in Barrett metaplasia a condition in which the normally stratified squamous epithelium of the lower esophagus is replaced by a metaplastic columnar epithelium. The condition develops as a complication in approximately 10% of patients with chronic gastroesophageal reflux disease and predisposes to the development of esophageal adenocarcinoma.; Esophageal cancer (ESCR)
Subcellular location Cytoplasm, Nucleus, Nucleus, PML body, Endoplasmic reticulum, Mitochondrion matrix, Note=Interaction with BANP promotes nuclear localization, Recruited into PML bodies together with CHEK2, Translocates to mitochondria upon oxidative stress, Translocates to mitochondria in response to mitomycin C treatment (PubMed:27323408), SUBCELLULAR LOCATION: Isoform 1: Nucleus, Cytoplasm, Note=Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4, SUBCELLULAR LOCATION: Isoform 2: Nucleus, Cytoplasm, Note=Localized mainly in the nucleus with minor staining in the cytoplasm, SUBCELLULAR LOCATION: Isoform 3: Nucleus, Cytoplasm, Note=Localized in the nucleus in most cells but found in the cytoplasm in some cells, SUBCELLULAR LOCATION: Isoform 4: Nucleus, Cytoplasm, Note=Predominantly nuclear but translocates to the cytoplasm following cell stress, SUBCELLULAR LOCATION: Isoform 7: Nucleus, Cytoplasm, Note=Localized mainly in the nucleus with minor staining in the cytoplasm, SUBCELLULAR LOCATION: Isoform 8: Nucleus, Cytoplasm
Pathway MAPK signaling pathway
p53 signaling pathway
PI3K-Akt signaling pathway
Wnt signaling pathway
Apoptosis
Cell cycle
Cellular senescence
Cell ageing and metabolism
Sphingolipid signaling pathway
Neurotrophin signaling pathway

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