Mouse Interferon γ ,IFN-γ ELISA Kit

Instructions
Code CSB-E04578m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name interferon, gamma
Alternative Names IFG, IFI,
Abbreviation IFNG
Uniprot No. P01580
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 82-99  
1:2 Average % 97  
Range % 87-105  
1:4 Average % 95  
Range % 89-100  
1:8 Average % 90  
Range % 82-98  
Recovery
The recovery of mouse IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-93  
EDTA plasma (n=4) 99 92-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.889 2.785 2.837 2.670  
500 2.356 2.324 2.340 2.173  
250 1.571 1.614 1.593 1.426  
125 0.885 0.862 0.874 0.707  
62.5 0.569 0.542 0.556 0.389  
31.2 0.315 0.330 0.323 0.156  
15.6 0.254 0.247 0.251 0.084  
0 0.164 0.169 0.167    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Target Data

Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Subcellular Location Secreted
Protein Families Type II (or gamma) interferon family
Tissue Specificity Released primarily from activated T lymphocytes.
Database Links

KEGG: mmu:15978

STRING: 10090.ENSMUSP00000063800

UniGene: Mm.240327

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