Mouse Interferon γ ,IFN-γ ELISA Kit

Instructions
Code CSB-E04578m
Size 96T,5×96T,10×96T
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Product Details

Target Name interferon, gamma
Alternative Names Ifng ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
Abbreviation IFNG
Uniprot No. P01580
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 82-99  
1:2 Average % 97  
Range % 87-105  
1:4 Average % 95  
Range % 89-100  
1:8 Average % 90  
Range % 82-98  
Recovery
The recovery of mouse IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-93  
EDTA plasma (n=4) 99 92-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.889 2.785 2.837 2.670  
500 2.356 2.324 2.340 2.173  
250 1.571 1.614 1.593 1.426  
125 0.885 0.862 0.874 0.707  
62.5 0.569 0.542 0.556 0.389  
31.2 0.315 0.330 0.323 0.156  
15.6 0.254 0.247 0.251 0.084  
0 0.164 0.169 0.167    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Gene References into Functions
  1. Data show that interleukin-2 inducible T cell kinase (Itk)negatively regulates the development of nTh1 cells that express interferon-gamma (IFNgamma) in a T-bet transcription factor (Tbet) independent manner. PMID: 28406139
  2. demonstrate in vivo that the conditional overexpression of Ifng in metanephric mesenchymal (MM) progenitors results in renal agenesis or hypoplasia. Cell death was observed in and around the MM region of E10.5-11.5 mutants where Ifng was constitutively expressed during early kidney development and resulted in retardation of branching morphogenesis. Expression of Sall1 was decreased in the MM of mutant kidneys. PMID: 29771971
  3. Data confirm Ifng as robust supporter of immune responses against tumors; here, mice treated with Chlorella vulgaris probiotic supposed as anti-carcinogen against mammary tumor, instead tumors in treated group exhibit more malignant phenotype and lower peri-tumoral neutrophil and macrophage-to-lymphocyte infiltration ratio compared to control mice; decline in serum Ifng levels correlated with tumor growth. PMID: 28229276
  4. In interferon gamma (IFNgamma)-deficient mice, Akkermansia muciniphila is significantly increased and restoration of IFNgamma levels reduces A. muciniphila abundance. PMID: 27841267
  5. this study shows the positive effects of Ifng on the early-stage differentiation and negative effects on the calcification of primary osteoblasts in vitro PMID: 29438885
  6. these data support the novel concept that IFN-gamma can have a detrimental role in the pathogenesis of influenza through a restriction in innate lymphoid cell group II activity PMID: 28513592
  7. IFN-gamma-iNOS axis are an essential pathway in the pathogenesis of arenavirus hemorrhagic fever. PMID: 28826838
  8. IFN-gamma can promote cancer immunoevasion. (Review) PMID: 29283429
  9. IRF-1 may be at the nexus of the interplay between IFN-gamma and IL-6 in exacerbating a xenobiotic-induced inflammatory response, regulation of interferon responsive genes and autoimmunity PMID: 28453771
  10. an enhanced expression of PD-L1 was observed besides an increased production of IFN-gamma by TH2 cells. PMID: 28917991
  11. Phosphorylation of T-bet by RSK2 is required for IFNgamma expression for attenuation of colon cancer metastasis and growth. PMID: 29133416
  12. SNX8 mediates IFNG-triggered non-canonical signaling pathway and host defense against Listeria monocytogenes. PMID: 29180417
  13. The complex role of IFN-gamma in autoimmunity and cholangitis.IFN-gamma induces IL-30 production which suppresses IFN-gamma mediated liver inflammation. PMID: 27721424
  14. this study shows that IFN-gamma promotes transendothelial migration of CD4(+) T cells across the blood-brain barrier PMID: 28682305
  15. Addition of a disintegrin and metallopeptidase domain 17 (ADAM17) to the culture supernatant of stimulated splenocytes decreased Interferon-gamma (IFN-gamma) concentration. PMID: 27573075
  16. a significant increase in plasma levels of IL-2, IFN-g and TNF-g was revealed as assessed by ELISA. In conclusion, the results of the present study indicate that MENK has a cytotoxic effect on B16 melanoma cells in vitro and in vivo, and suggest a potential mechanism for these bioactivities. PMID: 28849104
  17. the protective effect on metastasis was lost upon patrolling monocyte or NK cell depletion, IL15 neutralization, or IFNgamma ablation. The combined analysis of these approaches allowed us to establish a hierarchy in which patrolling monocytes, making IL15 in response to primary tumors, activate NK cells and IFNg production that then inhibit lung metastasis formation. PMID: 28811289
  18. Interferon-gamma derived from cytotoxic lymphocytes directly enhances their motility and cytotoxicity. PMID: 28569770
  19. this paper shows that interferon-gamma deficiency protects against aging-related goblet cell loss PMID: 27623073
  20. IL-15 induces the activation and survival of effector immune cells that are necessary for its antitumoral activity; but, long-term exposure to IL-15 is associated with the development of important side effects mainly mediated by IFN-gamma-producing T-cells PMID: 27356750
  21. Arid5a deficiency resulted in decreased levels of IFN-gamma under Th1 cell conditions, in which T-box expressed in T cells (T-bet) mRNA expression was inhibited. PMID: 27671645
  22. High dilutions of antimony modulate cytokines production and macrophage - Leishmania (L.) amazonensis interaction in vitro.( PMID: 28092793
  23. these findings highlight critical roles for IFN-gamma and IL-27 in the mechanism of respiratory syncytial virus-induced exacerbation PMID: 29167232
  24. MyD88 signaling in myeloid and dendritic cells is dispensable for IFN-gamma-dependent control of type A F. tularensis infection. PMID: 28951422
  25. spleen-derived IFN-gamma induces generation of PD-L1(+)-suppressive neutrophils. PMID: 28974543
  26. These results suggest that IL-17A plays an important role in host survival against Toxoplasma gondii infection by protecting the host from an anaphylactic reaction via the downregulation of Toxoplasma gondii HSP70 and IFN-gamma production. PMID: 28893913
  27. Stat3 mediates the expression of iNOS to promote IFNgamma/TNFalpha-induced muscle atrophy. PMID: 28264935
  28. NFAT5 can modulate different T-cell responses depending on stress conditions and stimulatory context. PMID: 27479742
  29. we identify interferon-gamma (IFN-gamma) as the key inflammatory mediator controlling sortilin-1 levels PMID: 28742217
  30. this study shows that IFN-gamma induction by neutrophil-derived IL-17A homodimer augments pulmonary antibacterial defense PMID: 26349661
  31. IFNG mediates experimental cerebral malaria by signaling within both the hematopoietic and nonhematopoietic compartments. PMID: 28874445
  32. identified TIM-4 as a novel marker for B effector 1 (Be1) cells that depend on IFN-gamma for their proinflammatory activity; TIM-4(+) B cells are enriched for IFN-gamma-producing proinflammatory Be1 cells that enhance immune responsiveness and can be specifically targeted with anti-TIM-4. PMID: 28848066
  33. results demonstrate that a population of Thy1.2(+) non-NK innate-like cells present in the liver expresses IFN-gamma and can confer protection against M. avium infection in immunocompromised mice PMID: 28687660
  34. Using IFN-gamma-deficient Th17 cells, study demonstrates the disease-amplifying role of Th17-derived IFN-gamma in dry eye disease pathogenesis. These results clearly demonstrate that Th17 cells mediate ocular surface autoimmunity through both IL-17A and IFN-gamma. PMID: 28637904
  35. IFNgamma is captured by phosphatidylserine (PS) on the surface of viable tumor cells both in vitro and in vivo then slowly released to drive long-term transcription of cytokine-response genes. PMID: 28575659
  36. Egr2 and 3 were essential to suppress Th1 differentiation in Th2 and Th17 conditions in vitro and also to control IFN-gamma-producing CD4 and CD8 T cells in response to virus infection PMID: 28455436
  37. intravital microscopy revealed IFNgamma-induced regression of the tumour vasculature, resulting in arrest of blood flow and subsequent collapse of tumours, similar to non-haemorrhagic necrosis in ischaemia PMID: 28445461
  38. studies identify the requirement of IFN-gamma stimulation as a mechanism for BC-CML and AML GVL resistance, whereas independence from IFN-gamma renders CP-CML more GVL sensitive, even with a lower-level alloimmune response. PMID: 28604385
  39. Results demonstrate that the influence of IFN-gamma on acute lymphoblastic leukemia progression may not be mediated by selection of nascent transformed cells but rather through a general suppressor of cytokine signaling -mediated reduction in B-cell precursor proliferation. PMID: 28295300
  40. The results indicate that interferon-gamma is critical for the initial priming and differentiation of cytotoxic T lymphocytes residing in the periphery to produce the most effect antitumor function within the eye. PMID: 26578649
  41. The authors showed the synergistic negative regulation of the pro-inflammatory cytokine interferon-gamma (IFNgamma) and beta3 integrin signaling in murine hematopoietic stem cells function by a novel definitive phenotyping of hematopoietic stem cells. PMID: 28673932
  42. We revealed that in mouse mesenchymal stem cells IFN-gamma-induced immunoregulation is mediated by early phosphorylation of signal transducer and activator of transcription (STAT) 1 and STAT3, which is significantly enhanced by an extracellular signal-regulated kinase 1/2-dependent mTOR inhibition, thereby promoting pSTAT1 nuclear translocation. PMID: 27670240
  43. this study showsthat the nature of the protective memory response depends closely on the route of infection and highlights the role of IFN-gamma-and IL-17RA-mediated responses in the control of mucosal infection by Brucella PMID: 27036913
  44. Study showed that the intraperitoneal administration of the exogenous cytokines IFN-gamma (to promote M1 microglia ) and IL-4 (to promote M2 microglia) can correctly modulate the timing of the M1 to M2 ratio to affect epileptogenesis and to improve cognitive function in pilocarpine-induced status epilepticus. PMID: 27956120
  45. findings show that bystander activation of T cells through TLRs leads to a short but biologically significant burst of IFN-gamma production, which relies exclusively on newly synthetized transcripts and energy generated by mitochondrial respiration PMID: 27016606
  46. Study finds that a high proportion of intratumoral Nrp1-/- Tregs produce interferon-gamma (IFNgamma), which drives the fragility of surrounding wild-type Tregs, boosts anti-tumor immunity, and facilitates tumor clearance. PMID: 28552348
  47. results demonstrate that Th1 cell-driven responses in the intestine during chronic helminth infection potently influence upstream hematopoietic processes in the Bone Marrow via IFN-gamma. PMID: 27594558
  48. Data show that lactic acid in tumor microenvironments inhibited interferon-gamma (IFNgamma) and intert=leukin-4 (IL4) productions from NKT cells by inhibiting mammalian target of rapamycin (mTOR) signaling. PMID: 27995420
  49. These findings reveal an unexpected tolerogenic role for IFN-gamma during viral persistence in the liver, providing new mechanistic insights regarding the maintenance of systemic antigen-specific tolerance during HBV persistence. PMID: 27139489
  50. CD4+CD25+ regulatory T cells promoted IFN-gamma and IL-17 mycoplasma-specific CD4+ T cell responses in vitro and in vivo. PMID: 27175511
  51. the translatability of the IFNgamma-biomarker correlates from mouse to human, advocating the use of serum CXCL9 or CXCL10 as a means to monitor total IFNgamma activity in patients with secondary hemophagocytic lymphohistiocytosis. PMID: 27559680
  52. this study shows that IFN-gamma may be used to stimulate the immunomodulatory property of Wharton's jelly mesenchymal stem cells PMID: 27697286
  53. In biliary atresia, miR-155 regulates the IFN-gamma signaling pathway by targeting SOCS1 expression. PMID: 27817193
  54. The reduction in fibrosis observed when IL-13 signalling is suppressed is not dependent on increased IFN-gamma activity. Instead, by reducing compensatory increases in type 1-associated inflammation, therapeutic strategies that block IFN-gamma and IL-13 activity simultaneously can confer greater protection from progressive fibrosis than IL-13 blockade alone. PMID: 27125685
  55. Results indicate that rather than marking the most proinflammatory lymphocytes in diabetes development, IFN-gamma production could represent an attempted limitation of pathogenic CD8(+) T-cell activation. PMID: 27920091
  56. The presence of nterferon-gamma in the lung protected from infection, but when delivered to the periphery, interferon-gamma enhanced disease, potentially by dampening inflammatory cytokines. PMID: 27068091
  57. These results indicate that induction of unfolded protein response by IFN-gamma is an important cause of goblet cell-associated mucin deficiency observed in aqueous-deficient dry eye PMID: 27085137
  58. IFN-gamma signalling increases levels of central nervous system pro-inflammatory cytokines. PMID: 27667782
  59. this study shows that diosgenin-mediated anti-allergic effects are associated with increased number of Foxp3+ Treg cells expressing IFN-g PMID: 27886644
  60. Vertical sleeve gastrectomy induces a weight loss in obese mice by increasing IFN-gamma secretion in mesenteric lymph nodes, which then increases the FXR expression in the liver and small intestine. PMID: 27376807
  61. PTK6 may act as a novel mediator of TNFalpha/IFNgamma intestinal epithelial permeability during inflammatory injury, and miR-93 may protect intestinal epithelial barrier function, at least in part, by targeting PTK6 PMID: 27119373
  62. Ellagic acid treatment augmented specific IL-10 production in response to S. mansoni antigenic stimulation. However, specific IL-1beta, IL-4, IL-12, IL-13, IL-17A, TNF-alpha and IFN-gamma production were significantly reduced with ex vivo and in vivo ellagic acid treatment. PMID: 27240831
  63. Flavonoid glycosides of Alchornea floribunda increased the proportion of splenic CD8(+)/IFNgamma(+ )and CD4(+)/IFNgamma(+ )T lymphocytes. PMID: 26974045
  64. These results indicate that Bhlhe40 works as a cofactor of T-bet for enhancing IFN-gamma production in iNKT cells. PMID: 27226296
  65. The production of IFNgamma finally accelerated the deposition of Abeta1-42 in beta-amyloid plaques. PMID: 26869183
  66. TNF-alpha together with IFN-gamma increased the proliferation of mesenchymal stem cells and slightly enhanced the expression of pluripotency genes. PMID: 26619160
  67. Intestinal CD103(+)CD11b(-) dendritic cells restrain colitis via IFN-gamma-induced anti-inflammatory response in epithelial cells. PMID: 26174764
  68. Group B streptococcus induces a robust IFN-gamma response by CD4(+) T cells. PMID: 26989699
  69. The results demonstrated that IL-17-producing CD8+ T (Tc17) cells and IFN-gamma+CD8+ T (Tc1) cells were detected in the early stage of Graft-versus-host disease. PMID: 26647759
  70. IFNgamma-dependent expression of NOS2 in the brain contributes to BBB breakdown and early mortality in murine PM. PMID: 26418460
  71. these results suggest that IL-17 intensified IFN-gamma-induced NOS2 upregulation and NO production by increasing the transcription activity of p-STAT1(Y701) and NF-kappaB in RAW 264.7 cells. PMID: 26677135
  72. Baicalin exhibited anti-influenza virus A (H1N1) activity in vitro and in vivo as a potent inducer of IFN-g in major IFN-g producing cells. PMID: 26783516
  73. IFN-gamma-treated mesenchymal stem cells strongly inhibit IL-10 production by activated B cells by a mechanism requiring cell contact and involving the Cox-2 pathway. PMID: 26416211
  74. STAT4 has a pro-atherogenic role that is at least partially independent of Th1 cell-derived interferon-gamma PMID: 26386214
  75. STAT1 deficiency reprograms IFN signaling from priming toward suppression of TLR response via feedback regulation of STAT3. PMID: 26299368
  76. study implicates adaptive immune dysfunction, in particular IFN-gamma, in disorders characterized by social dysfunction and suggests a co-evolutionary link between social behaviour and an anti-pathogen immune response driven by IFN-gamma signalling PMID: 27409813
  77. Results indicate that production of interferon-gamma (IFN-gamma) is a predominant protective mechanism conferred by CD8(+) T cells in the course of neosporosis. PMID: 26449650
  78. TRIF mediates antibacterial defense during Gram-negative pneumonia, at least in part, by inducing IFN-x03B3; at the primary site of infection. PMID: 26065469
  79. IFNgamma is critical for neonatal RSV clearance and it depends, in part, on alveolar macrophages for efficient viral clearing effects. PMID: 26438053
  80. Influenza H1N1 virus NS2 protein inhibits the production of interferon gamma in lung tissues. PMID: 26648293
  81. serum IFN-gamma correlates with increased protection, and our observation suggests Ifng+ T cells are maintained by cellular division PMID: 26646149
  82. Interferon-gamma is important in early defense against Mycobacterium ulcerans infection. PMID: 26863011
  83. Our findings may suggest that IFN-gamma production is one of the factors contributing to improved renal function after alphaGalCer administration. PMID: 26101952
  84. IFN-gamma induced or already present in tissues can impact downstream pleiotropic functions mediated by group 2 innate lymphoid cells. PMID: 26425820
  85. IL10 deficiency in CD4+ T cells enhances the C. rodentium-induced IL17 response and facilitates an IFNgamma response in the colon. PMID: 26160212
  86. Attenuation of experimental asthma by mycobacterial protein combined with CpG requires a TLR9-dependent IFN-gamma-CCR2 signalling circuit. PMID: 25944185
  87. These data demonstrate a role for IFN-gamma in aspects of both the innate and adaptive immune response to vaccinia virus and highlight the importance of NK cells in T cell-independent control of vaccinia virus in the respiratory tract. PMID: 26468539
  88. A role of interferon-gamma in limiting tissue-specific autoimmune disease.Natural killer cells-dendritic cells interaction elicits a positive feedback loop for IFN-gamma and IL-27 production. PMID: 26347474
  89. novel roles for Ifngamma and Nos2 in regulating Actin, Tubulin, CD11b, motility and morphology during the aggregation response of adherent peritoneal exudate cells PMID: 26029930
  90. IFN-gamma responses in the stomach of sublingually immunized mice promote vaccine-induced gastritis, after infection with H. pylori but that IL-17A primarily functions to reduce the bacterial load. PMID: 26168305
  91. Mouse models investigating the role of IFN-gamma as a mediator of hematopoietic cell apoptosis and destruction support IFN-gamma as a stimulator of Fas-mediated hematopoietic cell apoptosis. PMID: 26491068
  92. Phenolic Compounds from the Roots of Rhodiola crenulata and Their Antioxidant and Inducing IFN-gamma Production Activities. PMID: 26225952
  93. MMP-12 up-regulation mediated by SARM-TRIF signaling pathway contributes to IFN-gamma-independent airway inflammation and AHR post RSV infection in nude mice. PMID: 25652021
  94. Interferon-gamma-mediated allograft rejection exacerbates cardiovascular disease of hyperlipidemic murine transplant recipients. PMID: 26399469
  95. We concluded that H. pylori Omp18 has an important function influencing IFN-gamma-mediated immune response to participate in persistent colonization. PMID: 25945338
  96. The amelioration by Gl-PS against the suppression of the production of IL-2, IFN-gamma and TNF-alpha in mononuclear lymphocytes by B16F10 cell culture supernatant might contribute to cancer control. PMID: 25585987
  97. MicroRNA-181b alleviates acute graft versus host disease by down-regulating IFN-gamma production. PMID: 26223969
  98. A possible important role of Nfkbiz in modulating the progression of inflammatory diseases in which IFN-gamma and IL-1 are abundant. PMID: 25876660
  99. Data show that interferon gamma (IFN-gamma)-induced tau (tau) hyperphosphorylation was associated with release of the inhibitory effect of glycogen synthase kinase 3beta function. PMID: 26156074
  100. Data suggest a protective role for interferon gamma (IFN-gamma) in experimental autoimmune encephalomyelitis (EAE) by regulating the removal of myelin debris by CNS antigen-presenting cells (APCs). PMID: 26305941

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Subcellular Location Secreted
Protein Families Type II (or gamma) interferon family
Tissue Specificity Released primarily from activated T lymphocytes.
Database Links

KEGG: mmu:15978

STRING: 10090.ENSMUSP00000063800

UniGene: Mm.240327

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