Mouse Interferon γ ,IFN-γ ELISA Kit

Code CSB-E04578m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This ELISA kit is used for quantitative detection of mouse IFN-γ, establishing normal detection range and validation of antibody array results. It can measure IFN-γ levels in various biological fluids, including serum, plasma, tissue homogenates, or cell culture supernates. IFN-γ is a pleiotropic molecule that exerts cytostatic, antiproliferative, pro-apoptotic, and antitumor effects. Studies also showed that IFN-γ may also stimulate pro-tumorigenic transformations and promote tumor progression. Its pleiotropic functions are mediated by cell-specific expression of hundreds of IFN-γ-regulated genes that encompass inflammatory signaling molecules, apoptosis and cell cycle regulators, and transcriptional activators.

Based on the Sandwich-ELISA principle and enzyme-substrate chromogenic reaction property, the users can measure the content of mouse IFN-γ in serum, plasma, tissue homogenates, or cell culture supernates. This mouse IFN-γ ELISA kit is quality tested with high specificity, excellent sensitivity, precision less than 10%, good linearity, and high recovery. Click the product instructions for more validation information.

Target Name interferon, gamma
Alternative Names Ifng ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
Abbreviation IFNG
Uniprot No. P01580
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 82-99  
1:2 Average % 97  
Range % 87-105  
1:4 Average % 95  
Range % 89-100  
1:8 Average % 90  
Range % 82-98  
The recovery of mouse IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-93  
EDTA plasma (n=4) 99 92-107  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.889 2.785 2.837 2.670  
500 2.356 2.324 2.340 2.173  
250 1.571 1.614 1.593 1.426  
125 0.885 0.862 0.874 0.707  
62.5 0.569 0.542 0.556 0.389  
31.2 0.315 0.330 0.323 0.156  
15.6 0.254 0.247 0.251 0.084  
0 0.164 0.169 0.167    
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse IFN-γ This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • Biotin-labeled IFN-γ antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
  • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • Biotin-antibody Diluent 1 x 15 ml ---Dilute the Biotin-antibody solution.
  • HRP-avidin Diluent 1 x 15 ml ---Dilute the HRP-avidin solution.
  • Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
  • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
  • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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Target Data

Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Gene References into Functions
  1. Data show that interleukin-2 inducible T cell kinase (Itk)negatively regulates the development of nTh1 cells that express interferon-gamma (IFNgamma) in a T-bet transcription factor (Tbet) independent manner. PMID: 28406139
  2. demonstrate in vivo that the conditional overexpression of Ifng in metanephric mesenchymal (MM) progenitors results in renal agenesis or hypoplasia. Cell death was observed in and around the MM region of E10.5-11.5 mutants where Ifng was constitutively expressed during early kidney development and resulted in retardation of branching morphogenesis. Expression of Sall1 was decreased in the MM of mutant kidneys. PMID: 29771971
  3. Data confirm Ifng as robust supporter of immune responses against tumors; here, mice treated with Chlorella vulgaris probiotic supposed as anti-carcinogen against mammary tumor, instead tumors in treated group exhibit more malignant phenotype and lower peri-tumoral neutrophil and macrophage-to-lymphocyte infiltration ratio compared to control mice; decline in serum Ifng levels correlated with tumor growth. PMID: 28229276
  4. In interferon gamma (IFNgamma)-deficient mice, Akkermansia muciniphila is significantly increased and restoration of IFNgamma levels reduces A. muciniphila abundance. PMID: 27841267
  5. this study shows the positive effects of Ifng on the early-stage differentiation and negative effects on the calcification of primary osteoblasts in vitro PMID: 29438885
  6. these data support the novel concept that IFN-gamma can have a detrimental role in the pathogenesis of influenza through a restriction in innate lymphoid cell group II activity PMID: 28513592
  7. IFN-gamma-iNOS axis are an essential pathway in the pathogenesis of arenavirus hemorrhagic fever. PMID: 28826838
  8. IFN-gamma can promote cancer immunoevasion. (Review) PMID: 29283429
  9. IRF-1 may be at the nexus of the interplay between IFN-gamma and IL-6 in exacerbating a xenobiotic-induced inflammatory response, regulation of interferon responsive genes and autoimmunity PMID: 28453771
  10. an enhanced expression of PD-L1 was observed besides an increased production of IFN-gamma by TH2 cells. PMID: 28917991
  11. Phosphorylation of T-bet by RSK2 is required for IFNgamma expression for attenuation of colon cancer metastasis and growth. PMID: 29133416
  12. SNX8 mediates IFNG-triggered non-canonical signaling pathway and host defense against Listeria monocytogenes. PMID: 29180417
  13. The complex role of IFN-gamma in autoimmunity and cholangitis.IFN-gamma induces IL-30 production which suppresses IFN-gamma mediated liver inflammation. PMID: 27721424
  14. this study shows that IFN-gamma promotes transendothelial migration of CD4(+) T cells across the blood-brain barrier PMID: 28682305
  15. Addition of a disintegrin and metallopeptidase domain 17 (ADAM17) to the culture supernatant of stimulated splenocytes decreased Interferon-gamma (IFN-gamma) concentration. PMID: 27573075
  16. a significant increase in plasma levels of IL-2, IFN-g and TNF-g was revealed as assessed by ELISA. In conclusion, the results of the present study indicate that MENK has a cytotoxic effect on B16 melanoma cells in vitro and in vivo, and suggest a potential mechanism for these bioactivities. PMID: 28849104
  17. the protective effect on metastasis was lost upon patrolling monocyte or NK cell depletion, IL15 neutralization, or IFNgamma ablation. The combined analysis of these approaches allowed us to establish a hierarchy in which patrolling monocytes, making IL15 in response to primary tumors, activate NK cells and IFNg production that then inhibit lung metastasis formation. PMID: 28811289
  18. Interferon-gamma derived from cytotoxic lymphocytes directly enhances their motility and cytotoxicity. PMID: 28569770
  19. this paper shows that interferon-gamma deficiency protects against aging-related goblet cell loss PMID: 27623073
  20. IL-15 induces the activation and survival of effector immune cells that are necessary for its antitumoral activity; but, long-term exposure to IL-15 is associated with the development of important side effects mainly mediated by IFN-gamma-producing T-cells PMID: 27356750
  21. Arid5a deficiency resulted in decreased levels of IFN-gamma under Th1 cell conditions, in which T-box expressed in T cells (T-bet) mRNA expression was inhibited. PMID: 27671645
  22. High dilutions of antimony modulate cytokines production and macrophage - Leishmania (L.) amazonensis interaction in vitro.( PMID: 28092793
  23. these findings highlight critical roles for IFN-gamma and IL-27 in the mechanism of respiratory syncytial virus-induced exacerbation PMID: 29167232
  24. MyD88 signaling in myeloid and dendritic cells is dispensable for IFN-gamma-dependent control of type A F. tularensis infection. PMID: 28951422
  25. spleen-derived IFN-gamma induces generation of PD-L1(+)-suppressive neutrophils. PMID: 28974543
  26. These results suggest that IL-17A plays an important role in host survival against Toxoplasma gondii infection by protecting the host from an anaphylactic reaction via the downregulation of Toxoplasma gondii HSP70 and IFN-gamma production. PMID: 28893913
  27. Stat3 mediates the expression of iNOS to promote IFNgamma/TNFalpha-induced muscle atrophy. PMID: 28264935
  28. NFAT5 can modulate different T-cell responses depending on stress conditions and stimulatory context. PMID: 27479742
  29. we identify interferon-gamma (IFN-gamma) as the key inflammatory mediator controlling sortilin-1 levels PMID: 28742217
  30. this study shows that IFN-gamma induction by neutrophil-derived IL-17A homodimer augments pulmonary antibacterial defense PMID: 26349661
  31. IFNG mediates experimental cerebral malaria by signaling within both the hematopoietic and nonhematopoietic compartments. PMID: 28874445
  32. identified TIM-4 as a novel marker for B effector 1 (Be1) cells that depend on IFN-gamma for their proinflammatory activity; TIM-4(+) B cells are enriched for IFN-gamma-producing proinflammatory Be1 cells that enhance immune responsiveness and can be specifically targeted with anti-TIM-4. PMID: 28848066
  33. results demonstrate that a population of Thy1.2(+) non-NK innate-like cells present in the liver expresses IFN-gamma and can confer protection against M. avium infection in immunocompromised mice PMID: 28687660
  34. Using IFN-gamma-deficient Th17 cells, study demonstrates the disease-amplifying role of Th17-derived IFN-gamma in dry eye disease pathogenesis. These results clearly demonstrate that Th17 cells mediate ocular surface autoimmunity through both IL-17A and IFN-gamma. PMID: 28637904
  35. IFNgamma is captured by phosphatidylserine (PS) on the surface of viable tumor cells both in vitro and in vivo then slowly released to drive long-term transcription of cytokine-response genes. PMID: 28575659
  36. Egr2 and 3 were essential to suppress Th1 differentiation in Th2 and Th17 conditions in vitro and also to control IFN-gamma-producing CD4 and CD8 T cells in response to virus infection PMID: 28455436
  37. intravital microscopy revealed IFNgamma-induced regression of the tumour vasculature, resulting in arrest of blood flow and subsequent collapse of tumours, similar to non-haemorrhagic necrosis in ischaemia PMID: 28445461
  38. studies identify the requirement of IFN-gamma stimulation as a mechanism for BC-CML and AML GVL resistance, whereas independence from IFN-gamma renders CP-CML more GVL sensitive, even with a lower-level alloimmune response. PMID: 28604385
  39. Results demonstrate that the influence of IFN-gamma on acute lymphoblastic leukemia progression may not be mediated by selection of nascent transformed cells but rather through a general suppressor of cytokine signaling -mediated reduction in B-cell precursor proliferation. PMID: 28295300
  40. The results indicate that interferon-gamma is critical for the initial priming and differentiation of cytotoxic T lymphocytes residing in the periphery to produce the most effect antitumor function within the eye. PMID: 26578649
  41. The authors showed the synergistic negative regulation of the pro-inflammatory cytokine interferon-gamma (IFNgamma) and beta3 integrin signaling in murine hematopoietic stem cells function by a novel definitive phenotyping of hematopoietic stem cells. PMID: 28673932
  42. We revealed that in mouse mesenchymal stem cells IFN-gamma-induced immunoregulation is mediated by early phosphorylation of signal transducer and activator of transcription (STAT) 1 and STAT3, which is significantly enhanced by an extracellular signal-regulated kinase 1/2-dependent mTOR inhibition, thereby promoting pSTAT1 nuclear translocation. PMID: 27670240
  43. this study showsthat the nature of the protective memory response depends closely on the route of infection and highlights the role of IFN-gamma-and IL-17RA-mediated responses in the control of mucosal infection by Brucella PMID: 27036913
  44. Study showed that the intraperitoneal administration of the exogenous cytokines IFN-gamma (to promote M1 microglia ) and IL-4 (to promote M2 microglia) can correctly modulate the timing of the M1 to M2 ratio to affect epileptogenesis and to improve cognitive function in pilocarpine-induced status epilepticus. PMID: 27956120
  45. findings show that bystander activation of T cells through TLRs leads to a short but biologically significant burst of IFN-gamma production, which relies exclusively on newly synthetized transcripts and energy generated by mitochondrial respiration PMID: 27016606
  46. Study finds that a high proportion of intratumoral Nrp1-/- Tregs produce interferon-gamma (IFNgamma), which drives the fragility of surrounding wild-type Tregs, boosts anti-tumor immunity, and facilitates tumor clearance. PMID: 28552348
  47. results demonstrate that Th1 cell-driven responses in the intestine during chronic helminth infection potently influence upstream hematopoietic processes in the Bone Marrow via IFN-gamma. PMID: 27594558
  48. Data show that lactic acid in tumor microenvironments inhibited interferon-gamma (IFNgamma) and intert=leukin-4 (IL4) productions from NKT cells by inhibiting mammalian target of rapamycin (mTOR) signaling. PMID: 27995420
  49. These findings reveal an unexpected tolerogenic role for IFN-gamma during viral persistence in the liver, providing new mechanistic insights regarding the maintenance of systemic antigen-specific tolerance during HBV persistence. PMID: 27139489
  50. CD4+CD25+ regulatory T cells promoted IFN-gamma and IL-17 mycoplasma-specific CD4+ T cell responses in vitro and in vivo. PMID: 27175511

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Subcellular Location Secreted
Protein Families Type II (or gamma) interferon family
Tissue Specificity Released primarily from activated T lymphocytes.
Database Links

KEGG: mmu:15978

STRING: 10090.ENSMUSP00000063800

UniGene: Mm.240327

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