Mouse Interferon γ ,IFN-γ ELISA Kit

Code CSB-E04578m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01580
Lead Time 3-5 working days
Abbreviation IFNG
Protein Biological Process 1 Immunity
Target Name interferon, gamma
Alias IFG, IFI,
Species Mus musculus (Mouse)
Protein Biological Process 3 Antiviral defense
Sample Types serum, plasma, tissue homogenates, cell culture supernates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 5438
RGD 2866
MGI 107656
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 82-99  
1:2 Average % 97  
Range % 87-105  
1:4 Average % 95  
Range % 89-100  
1:8 Average % 90  
Range % 82-98  
Recovery
The recovery of mouse IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-93  
EDTA plasma (n=4) 99 92-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.889 2.785 2.837 2.670  
500 2.356 2.324 2.340 2.173  
250 1.571 1.614 1.593 1.426  
125 0.885 0.862 0.874 0.707  
62.5 0.569 0.542 0.556 0.389  
31.2 0.315 0.330 0.323 0.156  
15.6 0.254 0.247 0.251 0.084  
0 0.164 0.169 0.167    
References
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Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Subcellular Location Secreted
Protein Families Type II (or gamma) interferon family
Tissue Specificity Released primarily from activated T lymphocytes.
Database Links

KEGG: mmu:15978

STRING: 10090.ENSMUSP00000063800

UniGene: Mm.240327

Pathway HIF-1 signaling pathway
Jak-STAT signaling pathway
TGF-beta signaling pathway
Necroptosis
Antigen processing and presentation
IL-17 signaling pathway
Natural killer cell mediated cytotoxicity
Osteoclast differentiation
T cell receptor signaling pathway
Th1 and Th2 cell differentiation
Th17 cell differentiation

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