Mouse chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1) ELISA kit

Code CSB-E17286m
Size 96T,5×96T,10×96T
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Product Details

Target Name
chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1)
Alternative Names
Cxcl1 ELISA Kit; Gro ELISA Kit; Gro1 ELISA Kit; Mgsa ELISA Kit; Scyb1Growth-regulated alpha protein ELISA Kit; C-X-C motif chemokine 1 ELISA Kit; Platelet-derived growth factor-inducible protein KC ELISA Kit; Secretory protein N51) [Cleaved into: KC(5-72 ELISA Kit; Hematopoietic synergistic factor ELISA Kit; HSF ELISA Kit; KC-T)] ELISA Kit
Abbreviation
CXCL1
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 85-102  
1:2 Average % 89  
Range % 81-98  
1:4 Average % 95  
Range % 85-103  
1:8 Average % 96  
Range % 90-103  
Recovery
The recovery of mouse CXCL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 90-101  
EDTA plasma (n=4) 94 88-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 2.813 2.764 2.789 2.641  
250 2.010 1.988 1.999 1.851  
125 1.254 1.291 1.273 1.125  
62.5 0.747 0.782 0.765 0.617  
31.2 0.591 0.582 0.587 0.439  
15.6 0.326 0.317 0.322 0.174  
7.8 0.215 0.228 0.222 0.074  
0 0.145 0.151 0.148    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse CXCL1 ELISA Kit was designed for the quantitative measurement of Mouse CXCL1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

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Target Background

Function
(From Uniprot)
Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. KC(5-72) shows a highly enhanced hematopoietic activity.
Gene References into Functions
  1. Data suggest that CD4+ T lymphocytes and microglial CD40 mediate their pro-nociceptive effects in part by promoting selected chemokine responses, and more importantly, CXCL1 can play an anti-nociceptive role in peripheral nerve injury-induced neuropathic pain, which is possibly mediated by infiltrating neutrophils. PMID: 29309879
  2. Adipose stromal cells recruitment to tumours, driven by CXCL1 and CXCL8, promotes prostate cancer progression. PMID: 27241286
  3. The results demonstrate that C57BL/6J mice have a functional defect in NLRP12, impaired CXCL1 production, and that macrophages require NLRP12 expression for effective recruitment of neutrophils to inflammatory sites. PMID: 27779193
  4. GAG interactions and receptor activity of CXCL1 monomers and dimers are fine-tuned to regulate neutrophil trafficking for successful resolution of tissue injury. PMID: 27625115
  5. a paracrine role for Hippo-mediated secretion of CXCL1 and CXCL2 in the production of anti-microbial peptides (beta-defensins), iNOS, NOX2 and pro-inflammatory molecules during mycobacterial infection of the host, is reported. PMID: 27883091
  6. this study demonstrates that in vivo blocking of CXCL1 and CXCL2 can significantly reduce the Mycobacterium tuberculosis-induced bioactive IL-1beta production PMID: 28739876
  7. CXCL1 contributed to rapid neutrophil recruitment during Bacillus cereus endophthalmitis. In CXCL1-knockout mice, retinal function was greater and neutrophil influx was less than in control mice, confirming its role in ocular inflammation. PMID: 27286792
  8. Interferon-gamma (IFN-gamma) up-regulated interleukin 6 (IL-6) and CXCL1 chemokine (CXCL1) production of bone marrow mesenchymal stem cells (mBM-MSC). PMID: 28791835
  9. Our work deciphers the mCXCL1-mTORC1 pathway as crucial in liver cancer stem cell maintenance PMID: 27488521
  10. MMP7 shedding of syndecan-1/CXCL1 complexes functions as a checkpoint that restricts neutrophil activation at sites of epithelial injury. PMID: 26934670
  11. Data show that loss of loss of matrix metalloproteinase-3 (MMP-3) repressed the upregulation of the chemokines monocyte chemoattractant protein (MCP)-1 and (C-X-C motif) ligand 1 (CXCL1). PMID: 27809288
  12. The novel findings reveal the critical role of NLRP12-IL-17A-CXCL1 axis in host defense by modulating neutrophil recruitment against Klebsiella pneumoniae. PMID: 26349659
  13. Nlrp12 deficiency caused increased neutrophil migration towards the chemokine CXCL1 and the neutrophil parasite Leishmania major, revealing NLRP12 as a negative regulator of directed neutrophil migration under these conditions. PMID: 26514298
  14. Chemotactic activity of stellate cell-derived CXCL1 was assayed in vitro on neutrophils upon TLR4 activation. PMID: 27002851
  15. IL-17RA regulates CXL-1 and 5 production in the lungs during the adaptive response. PMID: 26871571
  16. Data suggest that CXCL1/IL-8, released from osteoclasts in an autoantibody-dependent manner, produces pain by activating sensory neurons. PMID: 26613766
  17. TGF-beta negatively regulates CXCL1 expression in CAFs through Smad2/3 binding to the promoter, and through suppression of HGF/c-Met autocrine signaling PMID: 26252654
  18. work shows parallel networks of necroptosis-induced CXCL1 and Mincle signalling that promote macrophage-induced adaptive immune suppression and thereby enable pancreatic ductal adenocarcinoma progression PMID: 27049944
  19. Repeated social defeat-induced VCAM-1 protein expression were localized to the vasculature of brain regions implicated in fear and anxiety responses. PMID: 25445193
  20. In a chronic-binge ethanol-feeding model, the hepatic TLR2 and TLR9-dependent MyD88-dependent pathway mediates CXCL1 production, resulting in the development of alcohol-mediated liver injury. PMID: 25930080
  21. IL-1 signaling plays a pivotal role in activating mucosal stromal cells to secrete CXCL1 which is essential for infiltration of IL-22-secreting neutrophils upon Citrobacter rodentium infection. PMID: 26034212
  22. Zfp30 is expressed in airway epithelia in the normal mouse lung and Zfp30 expression in vitro affects CXCL1 responses to an immune stimulus. PMID: 25114278
  23. Metoprolol also decreased serum levels of proinflammatory cytokines TNFalpha and CXCL1 and macrophage content in the plaques, showing that metoprolol has an anti-inflammatory effect. PMID: 25105129
  24. This study demonstrated the role of peripheral CXCL1/CXCR2 signaling in postoperative pain in the setting of prior morphine exposure. PMID: 24887006
  25. phagocytosis of refrigerator-stored RBCs in vivo induced increases in circulating monocyte chemoattractant protein-1 (MCP-1) and keratinocyte chemoattractant (KC) PMID: 25041478
  26. Neutrophil recruitment and the neutrophil cytokines, CXCL1/CXCL2, were suppressed in apo(a)transgenic mice in the abdominal aortic aneurysm model. PMID: 24650562
  27. These data identify a cytokine circuit that involves IL-1beta-induced production of CXCL1 and CXCL2 and leads the recruitment of neutrophils to streptococcal infection sites. PMID: 25114117
  28. Estrogen receptors alpha regulates CCL20/CXCL1 secretion in the female reproductive tract. PMID: 23025258
  29. These studies suggest that modulation of CXCL1 levels in tissues and blood could reduce bacterial burden in sepsis. PMID: 25172493
  30. Pharmacological inhibition of NAMPT activity mitigates inflammation in atherosclerotic plaques by reducing CXCL1-mediated activities on neutrophils. PMID: 24196571
  31. Data indicate that levels of toll-like receptors TLR4/4-stimulated chemokines CXCL1 and CXCL2 were selectively enhanced in stressed macrophages via receptor-interacting protein kinase 1 (RIPK1). PMID: 24920846
  32. findings demonstrated that CXCL1 and CXCL5 are increased in circulation with onset of T2D, are produced by islets under stress, and synergistically affect islet function, suggesting that these chemokines participate in pathogenesis of T2D. PMID: 24928936
  33. In vitro, neutrophils adherent to ICAM-1 or ICAM-2 rapidly released TNF in response to LTB4, C5a, and KC. PMID: 24913232
  34. Secretion of Cxcl1 (KC) cytokine is inhibited by electrical stimulation in early and late experimentally induced arthritic spleen. PMID: 23791889
  35. A sustained form of CXCL1 upregulation is demonstrated that can drive neuropathic pain in the spinal cord. PMID: 23831863
  36. the presence of CXCL1 is required for recurrent Herpetic stromal keratitis as evidenced by the lack of corneal disease in mice treated with anti-CXCL1 Ab. PMID: 24442436
  37. CXCL1 expressed by monocytes and CXCR2 on HBMEC is involved in monocytes migrating from blood to brain in AD patients PMID: 23967336
  38. BCG-triggered increase of lipid body biogenesis was inhibited by the PPARgamma antagonist GW9662, but not by the NF-kappaB inhibitor JSH-23. In contrast, KC/CXCL1 production was largely dependent on NF-kappaB but not on PPARgamma PMID: 24120921
  39. IL-17 and TNF-alpha synergistically mediate CXCL1 production by ATII cells after ischemia/reperfusion, via an NADPH oxidase-dependent mechanism. PMID: 24186876
  40. excessive CXCL1 expression in gp130-deficient endothelial cells augments neutrophil adhesion but hinders migration, most likely by disrupting chemotactic gradients PMID: 24081661
  41. KC, a potent neutrophil chemoattractant and an established angiogenic factor, failed to interfere in the post-infarction inflammatory response, in wound healing and scar formation after MI. PMID: 23598282
  42. Molecular basis of glycosaminoglycan heparin binding to the chemokine CXCL1 dimer. PMID: 23864653
  43. Overexpression and distinct localization of LCN2, CXCL1 and CXCL9 in the liver of fatty liver Shionogi mice suggest significant roles of these proteins in the pathogenesis of non-alcoholic steatohepatitis. PMID: 23875831
  44. At day 10, CIP treatment not only significantly reduced pro-inflammatory cytokine and chemokine concentrations, including interleukin-6 (IL-6) and KC, but it also enhanced IL-3 production compared to vehicle-treated controls. PMID: 23520506
  45. In severe S. aureus bacteraemia in mice, TNF-alpha, IL-1alpha, and KC are biomarkers predicting fatal outcome of infection. PMID: 23520553
  46. the model that mast cells, optimally positioned in close proximity to the vasculature, initiate an early phase of neutrophil recruitment by releasing the chemoattractants CXCL1/CXCL2. PMID: 23645836
  47. When expressed, CXCL1 is limited to small areas with faint staining and PCa progression does not rely on CXCL1 expression. PMID: 23334998
  48. MMP9 expressed by intestinal epithelial cells mediates inflammation in colitis with simultaneous increase in proinflammatory cytokine Kc. PMID: 23471340
  49. Early systemic release of IL-6 and keratinocyte-derived chemokine Cxcl1 may not necessarily predict an unfavorable outcome in this model of stroke. PMID: 22533966
  50. Intrahepatic CXCL1 & circulating CXCL1 were strongly induced shortly after islet infusion. Blockade of the CXCL1-CXCR1/2 axis in mice improved intrahepatic islet engraftment. The CXCR1/2-mediated pathway is a regulator of islet damage. PMID: 22996693

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Subcellular Location
Secreted.
Protein Families
Intercrine alpha (chemokine CxC) family
Database Links
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