Mouse chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1) ELISA kit

Instructions
Code CSB-E17286m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1)
Alternative Names Cxcl1 ELISA Kit; Gro ELISA Kit; Gro1 ELISA Kit; Mgsa ELISA Kit; Scyb1Growth-regulated alpha protein ELISA Kit; C-X-C motif chemokine 1 ELISA Kit; Platelet-derived growth factor-inducible protein KC ELISA Kit; Secretory protein N51) [Cleaved into: KC(5-72 ELISA Kit; Hematopoietic synergistic factor ELISA Kit; HSF ELISA Kit; KC-T)] ELISA Kit
Abbreviation CXCL1
Uniprot No. P12850
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CXCL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 85-102  
1:2 Average % 89  
Range % 81-98  
1:4 Average % 95  
Range % 85-103  
1:8 Average % 96  
Range % 90-103  
Recovery
The recovery of mouse CXCL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 90-101  
EDTA plasma (n=4) 94 88-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 2.813 2.764 2.789 2.641  
250 2.010 1.988 1.999 1.851  
125 1.254 1.291 1.273 1.125  
62.5 0.747 0.782 0.765 0.617  
31.2 0.591 0.582 0.587 0.439  
15.6 0.326 0.317 0.322 0.174  
7.8 0.215 0.228 0.222 0.074  
0 0.145 0.151 0.148    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation (By similarity). Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. KC(5-72) shows a highly enhanced hematopoietic activity.
Gene References into Functions
  1. Data suggest that CD4+ T lymphocytes and microglial CD40 mediate their pro-nociceptive effects in part by promoting selected chemokine responses, and more importantly, CXCL1 can play an anti-nociceptive role in peripheral nerve injury-induced neuropathic pain, which is possibly mediated by infiltrating neutrophils. PMID: 29309879
  2. Adipose stromal cells recruitment to tumours, driven by CXCL1 and CXCL8, promotes prostate cancer progression. PMID: 27241286
  3. The results demonstrate that C57BL/6J mice have a functional defect in NLRP12, impaired CXCL1 production, and that macrophages require NLRP12 expression for effective recruitment of neutrophils to inflammatory sites. PMID: 27779193
  4. GAG interactions and receptor activity of CXCL1 monomers and dimers are fine-tuned to regulate neutrophil trafficking for successful resolution of tissue injury. PMID: 27625115
  5. a paracrine role for Hippo-mediated secretion of CXCL1 and CXCL2 in the production of anti-microbial peptides (beta-defensins), iNOS, NOX2 and pro-inflammatory molecules during mycobacterial infection of the host, is reported. PMID: 27883091
  6. this study demonstrates that in vivo blocking of CXCL1 and CXCL2 can significantly reduce the Mycobacterium tuberculosis-induced bioactive IL-1beta production PMID: 28739876
  7. CXCL1 contributed to rapid neutrophil recruitment during Bacillus cereus endophthalmitis. In CXCL1-knockout mice, retinal function was greater and neutrophil influx was less than in control mice, confirming its role in ocular inflammation. PMID: 27286792
  8. Interferon-gamma (IFN-gamma) up-regulated interleukin 6 (IL-6) and CXCL1 chemokine (CXCL1) production of bone marrow mesenchymal stem cells (mBM-MSC). PMID: 28791835
  9. Our work deciphers the mCXCL1-mTORC1 pathway as crucial in liver cancer stem cell maintenance PMID: 27488521
  10. MMP7 shedding of syndecan-1/CXCL1 complexes functions as a checkpoint that restricts neutrophil activation at sites of epithelial injury. PMID: 26934670
  11. Data show that loss of loss of matrix metalloproteinase-3 (MMP-3) repressed the upregulation of the chemokines monocyte chemoattractant protein (MCP)-1 and (C-X-C motif) ligand 1 (CXCL1). PMID: 27809288
  12. The novel findings reveal the critical role of NLRP12-IL-17A-CXCL1 axis in host defense by modulating neutrophil recruitment against Klebsiella pneumoniae. PMID: 26349659
  13. Nlrp12 deficiency caused increased neutrophil migration towards the chemokine CXCL1 and the neutrophil parasite Leishmania major, revealing NLRP12 as a negative regulator of directed neutrophil migration under these conditions. PMID: 26514298
  14. Chemotactic activity of stellate cell-derived CXCL1 was assayed in vitro on neutrophils upon TLR4 activation. PMID: 27002851
  15. IL-17RA regulates CXL-1 and 5 production in the lungs during the adaptive response. PMID: 26871571
  16. Data suggest that CXCL1/IL-8, released from osteoclasts in an autoantibody-dependent manner, produces pain by activating sensory neurons. PMID: 26613766
  17. TGF-beta negatively regulates CXCL1 expression in CAFs through Smad2/3 binding to the promoter, and through suppression of HGF/c-Met autocrine signaling PMID: 26252654
  18. work shows parallel networks of necroptosis-induced CXCL1 and Mincle signalling that promote macrophage-induced adaptive immune suppression and thereby enable pancreatic ductal adenocarcinoma progression PMID: 27049944
  19. Repeated social defeat-induced VCAM-1 protein expression were localized to the vasculature of brain regions implicated in fear and anxiety responses. PMID: 25445193
  20. In a chronic-binge ethanol-feeding model, the hepatic TLR2 and TLR9-dependent MyD88-dependent pathway mediates CXCL1 production, resulting in the development of alcohol-mediated liver injury. PMID: 25930080
  21. IL-1 signaling plays a pivotal role in activating mucosal stromal cells to secrete CXCL1 which is essential for infiltration of IL-22-secreting neutrophils upon Citrobacter rodentium infection. PMID: 26034212
  22. Zfp30 is expressed in airway epithelia in the normal mouse lung and Zfp30 expression in vitro affects CXCL1 responses to an immune stimulus. PMID: 25114278
  23. Metoprolol also decreased serum levels of proinflammatory cytokines TNFalpha and CXCL1 and macrophage content in the plaques, showing that metoprolol has an anti-inflammatory effect. PMID: 25105129
  24. This study demonstrated the role of peripheral CXCL1/CXCR2 signaling in postoperative pain in the setting of prior morphine exposure. PMID: 24887006
  25. phagocytosis of refrigerator-stored RBCs in vivo induced increases in circulating monocyte chemoattractant protein-1 (MCP-1) and keratinocyte chemoattractant (KC) PMID: 25041478
  26. Neutrophil recruitment and the neutrophil cytokines, CXCL1/CXCL2, were suppressed in apo(a)transgenic mice in the abdominal aortic aneurysm model. PMID: 24650562
  27. These data identify a cytokine circuit that involves IL-1beta-induced production of CXCL1 and CXCL2 and leads the recruitment of neutrophils to streptococcal infection sites. PMID: 25114117
  28. Estrogen receptors alpha regulates CCL20/CXCL1 secretion in the female reproductive tract. PMID: 23025258
  29. These studies suggest that modulation of CXCL1 levels in tissues and blood could reduce bacterial burden in sepsis. PMID: 25172493
  30. Pharmacological inhibition of NAMPT activity mitigates inflammation in atherosclerotic plaques by reducing CXCL1-mediated activities on neutrophils. PMID: 24196571
  31. Data indicate that levels of toll-like receptors TLR4/4-stimulated chemokines CXCL1 and CXCL2 were selectively enhanced in stressed macrophages via receptor-interacting protein kinase 1 (RIPK1). PMID: 24920846
  32. findings demonstrated that CXCL1 and CXCL5 are increased in circulation with onset of T2D, are produced by islets under stress, and synergistically affect islet function, suggesting that these chemokines participate in pathogenesis of T2D. PMID: 24928936
  33. In vitro, neutrophils adherent to ICAM-1 or ICAM-2 rapidly released TNF in response to LTB4, C5a, and KC. PMID: 24913232
  34. Secretion of Cxcl1 (KC) cytokine is inhibited by electrical stimulation in early and late experimentally induced arthritic spleen. PMID: 23791889
  35. A sustained form of CXCL1 upregulation is demonstrated that can drive neuropathic pain in the spinal cord. PMID: 23831863
  36. the presence of CXCL1 is required for recurrent Herpetic stromal keratitis as evidenced by the lack of corneal disease in mice treated with anti-CXCL1 Ab. PMID: 24442436
  37. CXCL1 expressed by monocytes and CXCR2 on HBMEC is involved in monocytes migrating from blood to brain in AD patients PMID: 23967336
  38. BCG-triggered increase of lipid body biogenesis was inhibited by the PPARgamma antagonist GW9662, but not by the NF-kappaB inhibitor JSH-23. In contrast, KC/CXCL1 production was largely dependent on NF-kappaB but not on PPARgamma PMID: 24120921
  39. IL-17 and TNF-alpha synergistically mediate CXCL1 production by ATII cells after ischemia/reperfusion, via an NADPH oxidase-dependent mechanism. PMID: 24186876
  40. excessive CXCL1 expression in gp130-deficient endothelial cells augments neutrophil adhesion but hinders migration, most likely by disrupting chemotactic gradients PMID: 24081661
  41. KC, a potent neutrophil chemoattractant and an established angiogenic factor, failed to interfere in the post-infarction inflammatory response, in wound healing and scar formation after MI. PMID: 23598282
  42. Molecular basis of glycosaminoglycan heparin binding to the chemokine CXCL1 dimer. PMID: 23864653
  43. Overexpression and distinct localization of LCN2, CXCL1 and CXCL9 in the liver of fatty liver Shionogi mice suggest significant roles of these proteins in the pathogenesis of non-alcoholic steatohepatitis. PMID: 23875831
  44. At day 10, CIP treatment not only significantly reduced pro-inflammatory cytokine and chemokine concentrations, including interleukin-6 (IL-6) and KC, but it also enhanced IL-3 production compared to vehicle-treated controls. PMID: 23520506
  45. In severe S. aureus bacteraemia in mice, TNF-alpha, IL-1alpha, and KC are biomarkers predicting fatal outcome of infection. PMID: 23520553
  46. the model that mast cells, optimally positioned in close proximity to the vasculature, initiate an early phase of neutrophil recruitment by releasing the chemoattractants CXCL1/CXCL2. PMID: 23645836
  47. When expressed, CXCL1 is limited to small areas with faint staining and PCa progression does not rely on CXCL1 expression. PMID: 23334998
  48. MMP9 expressed by intestinal epithelial cells mediates inflammation in colitis with simultaneous increase in proinflammatory cytokine Kc. PMID: 23471340
  49. Early systemic release of IL-6 and keratinocyte-derived chemokine Cxcl1 may not necessarily predict an unfavorable outcome in this model of stroke. PMID: 22533966
  50. Intrahepatic CXCL1 & circulating CXCL1 were strongly induced shortly after islet infusion. Blockade of the CXCL1-CXCR1/2 axis in mice improved intrahepatic islet engraftment. The CXCR1/2-mediated pathway is a regulator of islet damage. PMID: 22996693
  51. circulating IL-6 contributes to acute kidney injury -mediated lung neutrophil accumulation and lung injury via upregulation of lung CXCL1. PMID: 22791336
  52. results indicate that PLCvarepsilon has a crucial role in UVB-induced acute inflammatory reactions such as neutrophil infiltration and epidermal thickening by at least in part regulating expression of CXCL1/KC in skin cells such as keratinocytes and fibroblasts PMID: 21321537
  53. CXCL1 controls neutrophil recruitment and regulates the expression of NADPH oxidase, iNOS & LTB4 in the lungs after K. pneumoniae infection. PMID: 22379035
  54. overexpression of CXCL1 attenuates diet-induced obesity, likely mediated through a CXCL1-induced improvement of fatty acid oxidation and oxidative capacity in skeletal muscle tissue PMID: 22275756
  55. Fasting significantly augments serum CXCL-1, and CXCL-1 expression in liver and muscle. PMID: 21224226
  56. Fas induces KC release in MLE-12 cells by a mechanism requiring MyD88, mitogen-activated protein kinases, and likely activator protein-1 PMID: 21257927
  57. Treatment with IL-17 prolongs the half-life of chemokine CXCL1 mRNA via the adaptor TRAF5 and the splicing-regulatory factor SF2 (ASF). PMID: 21822258
  58. major cause of a high irritant dermatitis to phthalic anhydride PMID: 20864798
  59. Overexpression of keratinocyte-derived chemokine (KC) in transgenic mice produced hyperplastic prostate epithelial acini associated with a periacinar reactive stroma [keratinocyte-derived chemokine]. PMID: 19021203
  60. Excessive C5a in sepsis functions as a critical inflammatory mediator to enhance IL-8 production mainly through MAPK signaling pathways. PMID: 20591742
  61. Rho-kinase signalling regulates TNF-alpha and CXC chemokine formation as well as lipid peroxidation in the reperfused colon. PMID: 20593289
  62. KC plays a critical nonredundant role in the development of experimental Lyme arthritis and carditis via CXCR2-mediated recruitment of neutrophils into the site of infection. PMID: 20823213
  63. The aim of this study was to determine whether acinar cells are a source of KC and MIP-2 and to understand their transcriptional regulation. PMID: 20671197
  64. Astrocytes from mice deficient in MyD88 signaling produced significantly less MCP-1 and MIP-2 and were unable to synthesize KC. PMID: 19932745
  65. Results show that chemokine induction by IDR-1002 was found to be mediated through a Gi-coupled receptor and the PI3K, NF-kappaB, and MAPK signaling pathways. PMID: 20107187
  66. The distribution of the functional IL-8 homologues CXCL1/KC, CXCL2/MIP-2, and CXCL5-6/LIX in resting and inflamed murine vessels, is examined. PMID: 20007247
  67. TLR2,4/LPS and TLR5/flagellin are necessary for the induction of KC, TNF-alpha, and IL-6 synthesis by P. aeruginosa-activated cells PMID: 19806220
  68. Migration of PMNs to the CNS coincided with increased expression of transcripts specific for the CXCR2 ELR-positive chemokine ligands CXCL1, CXCL2, and CXCL5 within the brain PMID: 19893623
  69. Data show that oral fibroblasts respond to LPS stimulation by increasing GROalpha production via the transcription factor NF-kappaB, suggesting that this mechanism may be involved in development of periodontal inflammation. PMID: 19430878
  70. neutrophil chemoattractant properties in a conditional transgenic model PMID: 11739532
  71. Neutrophil-directed KC chemokine expression predominates at the incisional site during the first 12 hrs following experimental surgical injury and declines 24 hrs after injury. PMID: 11907123
  72. matrilysin generates a transepithelial gradient of kc, a CXC chemokine PMID: 12464176
  73. Mast cell activation- and KC-induced neutrophil responses are LFA-1-dependent PMID: 12488493
  74. The chemokine KC is differentially expressed in joints of C57BL/6J and C3H mice and correlates with the development of experimental Lyme arthritis, mediating recruitment of neutrophils into the infected joint. PMID: 12847259
  75. CXC chemokines play a fundamental role in colonic ischemia/reperfusion and that functional interference with CXC chemokines may protect against pathological inflammation in the colon PMID: 14583342
  76. The PMN transendothelial migration induced by the activated myocytes is dependent on the production and secretion of the chemokines LIX and KC by the activated myocytes. PMID: 14988231
  77. Decreased expression of CXCL1 chemokines by aged B cells in response to LPS may have implications on the recruitment of leukocytes to sites of infections and inflammation possibly contributing to increased susceptibility of older subjects to pathogens. PMID: 15274748
  78. effects of TGF-beta on expression of the platelet-derived growth factor-induced KC protein were explored in mouse mesenchymal C3H10T1/2 and pre-osteoblastic MC3T3-E1 cells PMID: 15582587
  79. CXCL1-3/KC and CCL2/JE are generated within the sponge compartment and neovascularization and inflammatory cells influx can be modulated by exogenous administration of the chemokines PMID: 15597153
  80. induction of CXCL1/GRO1 is of importance for HA12-induced endothelial cell sprouting PMID: 15843382
  81. Macrophage inflammatory protein 2-alpha (MIP-2alpha) and KC differ in their mediation of neutrophil function (apoptosis and chemotaxis) and contribution to the pathogenesis of lung injury following hemorrhage subsequent to sepsis. PMID: 15961314
  82. KC (CXCL1) mRNA is regulated by functionally independent AU-rich sequence motifs PMID: 15994316
  83. Over expression of CXCL1 confers increased tumorigenicity to glioma cells PMID: 16033775
  84. Auroarimularion by recombinant proteins in mice with preexisting liver injury indicates a positive feedback regulation. PMID: 16118408
  85. Pathogenic, but not nonpathogenic, anti-DNA antibodies significantly induced a number of transcripts, including CXCL1/KC, LCN2, iNOS, CX3CL1/fractalkine, SERPINA3G, and IkappaBalpha ("marker genes"). PMID: 16804897
  86. production of the chemokine CXCL1 by cultured Theiler murine encephalomyelitis virus (TMEV)-infected astrocytes; propose an important role for CXCL1 in this experimental model of multiple sclerosis as a chemoattractant of destructive immune cells PMID: 16996102
  87. adenosine plays an important role, via CXCL1, in the induction of pulmonary angiogenesis PMID: 17227950
  88. Treatment of chimeras with TLR4 present only on intrinsic renal cells with anti-CXCL1 and anti-CXCL2 antibody before disease induction significantly reduced renal neutrophil infiltration in glomerulonephritis PMID: 17460147
  89. These results indicate that KC and MIP-2 play important roles in the infiltration of neutrophils into the site of injection of necrotic cells and that neutrophils may regulate monocyte/macrophage infiltration. PMID: 17662241
  90. upregulation of KC by Clara cells in the setting of LPS stimulation is largely dependent on TNF-alpha originating from alveolar macrophages. PMID: 17673686
  91. lumican and keratocan core proteins bind the CXC chemokine KC during a corneal inflammatory response, indicating that corneal KSPGs mediate neutrophil recruitment to the cornea by regulating chemokine gradient formation. PMID: 17911102
  92. Resident tissue macrophages are the major source of KC and macrophage-inflammatory protein (MIP)-2; these chemokines are newly synthesized products of signaling through Toll-like receptors. PMID: 18322244
  93. A Th17-ELR(+) CXC chemokine pathway is critical for granulocyte mobilization, blood-brain-barrier compromise, and the clinical manifestation of autoimmune demyelination. PMID: 18347102
  94. In vivo neutralization of CXCL1/KC represents a potential novel therapy that could be used to increase the survival rate of high-risk vascularized corneal allografts. PMID: 18347542
  95. role of CXCL1/KC in intestinal response to inflammation; findings imply that neutrophil infiltration induced by CXCL1/KC is an essential component of intestinal response to inflammatory stimuli & ability of intestine to restore mucosal barrier integrity PMID: 18713728
  96. These findings suggest a neuroprotective role for CXCL1 during the course of autoimmune demyelination PMID: 19095949
  97. The chemokine Cxcl1 is a novel target gene of parathyroid hormone (PTH)/PTH-related protein in committed osteoblasts. PMID: 19147675
  98. The lack of CXCR2 in hematopoietic cells is sufficient to protect from adipose and skeletal muscle macrophage recruitment and development of insulin resistance in diet-induced obese mice. PMID: 19494115

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Subcellular Location Secreted
Protein Families Intercrine alpha (chemokine CxC) family
Database Links

KEGG: mmu:14825

STRING: 10090.ENSMUSP00000031327

UniGene: Mm.21013

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