Mouse interleukin 10,IL-10 ELISA KIT

Code CSB-E04594m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P18893
Lead Time 3-5 working days
Abbreviation IL10
Target Name interleukin 10
Alias CSIF, IL-10, IL10A, MGC126450, MGC126451, TGIF, cytokine synthesis inhibitory factor
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 81-94  
1:2 Average % 96  
Range % 91-100  
1:4 Average % 96  
Range % 93-104  
1:8 Average % 89  
Range % 81-103  
Recovery
The recovery of mouse IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 81-98  
EDTA plasma (n=4) 94 91-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.272 2.152 2.212 2.068  
100 1.794 1.760 1.777 1.633  
50 1.290 1.194 1.242 1.098  
25 0.787 0.769 0.778 0.634  
12.5 0.493 0.487 0.490 0.346  
6.25 0.336 0.329 0.333 0.189  
3.12 0.236 0.219 0.228 0.084  
0 0.149 0.139 0.144    
References
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Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Subcellular Location Secreted
Protein Families IL-10 family
Database Links

KEGG: mmu:16153

STRING: 10090.ENSMUSP00000016673

UniGene: Mm.874

Pathway Jak-STAT signaling pathway
T cell receptor signaling pathway
FoxO signaling pathway

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