The Mouse luteinizing hormone (LH) ELISA kit is designed for detection and quantitation of human IL-1β in serum, plasma, or tissue homogenates. It has been validated with high sensitivity, strong specificity, good linearity, precision less than 15%, and consistency across batch. Refer to the product instructions for more details.
The measurement of this assay is based on the Sandwich-ELISA tecnique. The samples and biotin-labeled LH antibody are successively pipetted into the wells pre-coated with anti-mouse LH antibody. HRP-avidin is subsequently added to the wells. After thorough washing, the substrate TMB is added for color development. TMB is converted to blue by HRP and to yellow by the stop solution. The intensity of color is positively correlated with the LH content in the samples.
LH is a gonadotropin hormone released from the anterior pituitary. It stimulates the synthesis of sex steroids, which in turn negatively regulate the release of hypothalamic GnRH and further LH production. LH contributes to the maturation of primordial germ cells in both sexes. In male, LH causes the Leydig cells of the testes to produce testosterone. While LH triggers the creation of steroid hormones from the ovaries in female. LH is also involved in the modulation of the length and order of the menstrual cycle in females.