Pig insulin-like growth factors-1,IGF-1 ELISA Kit

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Pig insulin-like growth factors-1,IGF-1 ELISA Kit

Name: Pig insulin-like growth factors-1,IGF-1 ELISA Kit
Brand: CUSABIO
SKU: CSB-E06829p

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Code	CSB-E06829p
Size	96T,5×96T,10×96T
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Citations
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Target Data

Target Name

insulin-like growth factor 1 (somatomedin C)

Alternative Names

IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit

Abbreviation

IGF1

Uniprot No.

P16545

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates, cell lysates

Detection Range

6.25 ng/mL-400 ng/mL

Sensitivity

1.56 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-98
1:2	Average %	95
	Range %	90-104
1:4	Average %	97
	Range %	92-110
1:8	Average %	95
	Range %	82-100

Recovery

The recovery of pig IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	85-108
EDTA plasma (n=4)	95	91-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
400	2.824	2.815	2.820	2.676
200	2.362	2.346	2.354	2.210
100	1.469	1.489	1.479	1.335
50	0.709	0.684	0.697	0.553
25	0.416	0.425	0.421	0.277
12.5	0.251	0.262	0.257	0.113
6.25	0.199	0.192	0.196	0.052
0	0.146	0.142	0.144

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Effects of Moringa oleifera Leaf Meal on Plasma Ghrelin and Insulin-like Growth Factor-1 Levels in Swine and its Potential Role in Improving Sow Productivity RKEB Gonzales,Philippine Agricultural,2020
Excessive BCAA regulates fat metabolism partially through the modification of m6A RNA methylation in weanling piglets Heng J,Nutrition & Metabolism,2020
Maternal Diet-Induced Obesity Compromises Oxidative Stress Status and Angiogenesis in the Porcine Placenta by Upregulating Nox2 Expression Hu C, et al,Oxidative Medicine and Cellular Longevity,2019
Effect of optimizing dietary valine-to-lysine ratio in late gestation or lactation on biochemical indices and performance of lactating primiparous sows KaiguoGao,Animal Feed Science and Technology,2019
Potential Link between Gut Microbiota and Deoxynivalenol-Induced Feed Refusal in Weaned Piglets Wang S, et al,journal of agricultural and food chemistry,2019
Mechanistic insight into the role of immunocastration on eliminating skatole in boars Han X, et al,Theriogenology,2019
Effects of dietary methionine restriction on postnatal growth, insulin sensitivity, and glucose metabolism in intrauterine growth retardation pigs at 49 and 105 days of age Ying Z, et al,Journal of animal science,2018
Effects of dietary methionine restriction on postnatal growth, insulin sensitivity, and glucose metabolism in intrauterine growth retardation pigs at 49 and 105 days of age Ying Z, et al,Journal of animal science,2018
Ambroxol attenuates cisplatin-induced hepato and nephrotoxicity via inhibition of p-JNK/p-ERK A Bishr, et al,Journal of Functional Foods,2018
Chronic heat stress increases insulin-like growth factor-1(IGF-1) but does not affect IGF-binding proteins in growing pigs Hairui Xin, .et al,Journal of Thermal Biology,2018
Dietary Methionine Restriction Alleviates Hyperglycemia in Pigs with Intrauterine Growth Restriction by Enhancing Hepatic Protein Kinase B Signaling and Glycogen Synthesis Ying Z.et al,J Nutr.,2017
Transgenic Wuzhishan minipigs designed to express a dominant-negative porcine growth hormone receptor display small stature and a perturbed insulin/IGF-1 pathway Li F. et al,Transgenic Res,2015

Function	The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV
Gene References into Functions	High temperature, independent feed intake reduction, increases IGF-1:IGFBP-3 ratio. PMID: 30196890 the dipeptide Pro-Asp promoted IGF-1 secretion and expression in hepatocytes. PMID: 27473671 The immunoprecipitation results also showed that high AA concentrations significantly increased the interaction of mTOR and PPARg. In summary, PPARg plays an important role in the regulation of IGF-1 secretion and gene expression in response to dietary protein. PMID: 28257428 Data suggest that both ovarian follicular dominance in cows and cooperation of ovarian follicles in pigs can be mediated by either down- or up-regulation of the insulin-like growth factor 1/oxytocin system. PMID: 28163019 This study demonstrated that Up-regulation of IGF-1 in the frontal cortex of piglets exposed to an environmentally enriched arena. PMID: 28238777 Combined administration of mesenchymal stem cells overexpressing IGF-1 and HGF enhances neovascularization but moderately improves cardiac regeneration in a porcine model. PMID: 27423905 Results show that expression changes of IGF1 genes were associated with C/EBP b expression during embryonic and postnatal development in porcine liver. PMID: 25543525 genetic association study suggests that the TC genotype of IGF-1 represents the selection genotype for smaller in size. PMID: 24634183 IGF-I coordinately regulates multiple cell signaling pathways that are critical to proliferation, migration and survival of trophectoderm cells during early pregnancy in pigs. PMID: 24508636 IGF-1 splice variant (mechano growth factor) is expressed within the growth plate, but the addition of its peptides was not associated with growth plate chondrocytes proliferation. PMID: 24146828 alpha-linolenic acid increased IGF-I secretion from hepatocytes. PMID: 23053988 Copper can stimulate chondrocyte proliferation by promoting the expression of IGF-1. PMID: 22367704 The results show that modulation of plasma insulin levels by dietary carbohydrates seems possible in anabolic sows, but IGF-I levels are less easily modified. PMID: 21635573 The results suggested that IFG-1 and -2 and their receptors are differentially expressed at the maternal and fetal components of the attachment site. PMID: 21685713 Plasma ghrelin level and myocardial IGF-1 mRNA expression were significantly up-regulated, while plasma IGF-1 level and myocardial ghrelin mRNA expression were down-regulated in the chronic cyanotic immature piglets. PMID: 21933568 IGFI signaling pathway regulates myofibre hypertrophy postnatally via complicated signal effectors, which may have negative impact on meat quality simultaneously. PMID: 21246287 IGF-I, IGF-II, and IGFBP-3 mRNA were positively correlated with IGF-IR from 50E to 180D, suggesting that the expression of IGF-system genes exhibits specific developmental patterns in the skeletal muscle tissues. PMID: 21570979 These results indicate that sorbic acid improves growth performance by regulating IGF system gene expression and hormone secretion. PMID: 21421836 In an animal model of acute myocardial infarction relevant to human disease, intracoronary administration of IGF-1/hepatocyte growth factor (HGF) is a practical and effective strategy to reduce pathological cardiac remodeling. PMID: 21723061 The expression of IGF-I and IGF-II in native growth plates of prepubertal piglets and under different cell culture conditions, was compared. PMID: 20600587 The aims of this study were to investigate whether aldosterone influences IGF-I signaling and to determine the mechanism(s) by which aldosterone affects IGF-I function. PMID: 20881255 Data show that hepatocytes secreted IGF-I under basal conditions and this was enhanced by GH addition. PMID: 19786114 an important role of IGF1 in leptin receptor expression during the entire estrous cycle, with a special role during the prepubertal period PMID: 17139134 study concludes that IGF1 promotes the FSH-stimulated synthesis and retention of hyaluronic acid in pig oocyte-cumulus complexes by PIK3/AKT- and MAPK3/1-dependent mechanisms PMID: 17329596 Significant effect was found for IGF1 genotype on birth weight (P<0.05), with positive effects associated with the 286/286 genotype, and 280/286 genotype on litter weight at birth in second parity. PMID: 17369178 Both classes of IGF-I mRNA were expressed in a variety of tissues, however, class 1 IGF-I mRNA was more abundant than class 2 in all tissues. PMID: 18157703 in VSMCs exposed to hyperglycemia, IGF-I stimulation of Shc facilitates the transfer of Grb2 to p85 resulting in enhanced PI3K activation and AKT phosphorylation leading to enhanced cell proliferation and migration PMID: 18420583 These data demonstrated that feed allowance level has important consequence on embryo survival, embryo development and uterine development, possibly mediated by changes in concentrations of reproductive hormones. PMID: 18700853 Show More Hide All
Subcellular Location	Secreted
Protein Families	Insulin family
Database Links	KEGG: ssc:397491 STRING: 9823.ENSSSCP00000000915 UniGene: Ssc.16231

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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