Pig insulin-like growth factors-1,IGF-1 ELISA Kit

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Pig insulin-like growth factors-1,IGF-1 ELISA Kit

Specification
Target

Code

CSB-E06829p

See More Details

Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P16545

Lead Time

3-5 working days

Abbreviation

IGF1

Target Name

insulin-like growth factor 1 (somatomedin C)

Alias

IGF1A, IGFI, OTTHUMP00000195084|insulin-like growth factor 1

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates, cell lysates

Detection Range

6.25 ng/mL-400 ng/mL

Sensitivity

1.56 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Sandwich

HGNC

5464

RGD

2868

MGI

96432

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-98
1:2	Average %	95
	Range %	90-104
1:4	Average %	97
	Range %	92-110
1:8	Average %	95
	Range %	82-100

Recovery

The recovery of pig IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	85-108
EDTA plasma (n=4)	95	91-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
400	2.824	2.815	2.820	2.676
200	2.362	2.346	2.354	2.210
100	1.469	1.489	1.479	1.335
50	0.709	0.684	0.697	0.553
25	0.416	0.425	0.421	0.277
12.5	0.251	0.262	0.257	0.113
6.25	0.199	0.192	0.196	0.052
0	0.146	0.142	0.144

References

Transgenic Wuzhishan minipigs designed to express a dominant-negative porcine growth hormone receptor display small stature and a perturbed insulin/IGF-1 pathway Li F. et al,Transgenic Res,2015 24(6):1029-1042
Dietary Methionine Restriction Alleviates Hyperglycemia in Pigs with Intrauterine Growth Restriction by Enhancing Hepatic Protein Kinase B Signaling and Glycogen Synthesis Ying Z.et al,J Nutr.,Aug 23
Chronic heat stress increases insulin-like growth factor-1(IGF-1) but does not affect IGF-binding proteins in growing pigs Hairui Xin, .et al,Journal of Thermal Biology,August 18
Ambroxol attenuates cisplatin-induced hepato and nephrotoxicity via inhibition of p-JNK/p-ERK A Bishr, et al,Journal of Functional Foods,November 1
Effects of dietary methionine restriction on postnatal growth, insulin sensitivity, and glucose metabolism in intrauterine growth retardation pigs at 49 and 105 days of age Ying Z, et al,Journal of animal science,December 2
Effects of dietary methionine restriction on postnatal growth, insulin sensitivity, and glucose metabolism in intrauterine growth retardation pigs at 49 and 105 days of age Ying Z, et al,Journal of animal science,December 2

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Function	The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV
Subcellular Location	Secreted
Protein Families	Insulin family
Database Links	KEGG: ssc:397491 STRING: 9823.ENSSSCP00000000915 UniGene: Ssc.16231
Pathway	HIF-1 signaling pathway MAPK signaling pathway mTOR signaling pathway p53 signaling pathway PI3K-Akt signaling pathway Focal adhesion Ras signaling pathway Signaling pathways regulating pluripotency of stem cells AMPK signaling FoxO signaling pathway Rap1 signaling pathway

IGF1 Protein

IGF1 Proteins for Sus scrofa (Pig)

Code	Product Name	Source
CSB-YP011086PI CSB-EP011086PI CSB-BP011086PI CSB-MP011086PI	Recombinant Pig Insulin-like growth factor I(IGF1)	Yeast E.coli Baculovirus Mammalian cell

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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