Pig insulin-like growth factors-1,IGF-1 ELISA Kit

Code CSB-E06829p
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P16545
Lead Time 3-5 working days
Abbreviation IGF1
Target Name insulin-like growth factor 1 (somatomedin C)
Alias IGF1A, IGFI, OTTHUMP00000195084|insulin-like growth factor 1
Species Sus scrofa (Pig)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 5464
RGD 2868
MGI 96432
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 80-98  
1:2 Average % 95  
Range % 90-104  
1:4 Average % 97  
Range % 92-110  
1:8 Average % 95  
Range % 82-100  
Recovery
The recovery of pig IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 85-108  
EDTA plasma (n=4) 95 91-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
400 2.824 2.815 2.820 2.676  
200 2.362 2.346 2.354 2.210  
100 1.469 1.489 1.479 1.335  
50 0.709 0.684 0.697 0.553  
25 0.416 0.425 0.421 0.277  
12.5 0.251 0.262 0.257 0.113  
6.25 0.199 0.192 0.196 0.052  
0 0.146 0.142 0.144    
References
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Function The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV
Subcellular Location Secreted
Protein Families Insulin family
Database Links

KEGG: ssc:397491

STRING: 9823.ENSSSCP00000000915

UniGene: Ssc.16231

Pathway HIF-1 signaling pathway
MAPK signaling pathway
mTOR signaling pathway
p53 signaling pathway
PI3K-Akt signaling pathway
Focal adhesion
Ras signaling pathway
Signaling pathways regulating pluripotency of stem cells
AMPK signaling
FoxO signaling pathway
Rap1 signaling pathway

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