Pig insulin-like growth factors-1,IGF-1 ELISA Kit

Instructions
Code CSB-E06829p
Size 96T,5×96T,10×96T
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Product Details

Target Name insulin-like growth factor 1 (somatomedin C)
Alternative Names IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
Abbreviation IGF1
Uniprot No. P16545
Species Sus scrofa (Pig)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 80-98  
1:2 Average % 95  
Range % 90-104  
1:4 Average % 97  
Range % 92-110  
1:8 Average % 95  
Range % 82-100  
Recovery
The recovery of pig IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 85-108  
EDTA plasma (n=4) 95 91-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
400 2.824 2.815 2.820 2.676  
200 2.362 2.346 2.354 2.210  
100 1.469 1.489 1.479 1.335  
50 0.709 0.684 0.697 0.553  
25 0.416 0.425 0.421 0.277  
12.5 0.251 0.262 0.257 0.113  
6.25 0.199 0.192 0.196 0.052  
0 0.146 0.142 0.144    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV
Gene References into Functions
  1. High temperature, independent feed intake reduction, increases IGF-1:IGFBP-3 ratio. PMID: 30196890
  2. the dipeptide Pro-Asp promoted IGF-1 secretion and expression in hepatocytes. PMID: 27473671
  3. The immunoprecipitation results also showed that high AA concentrations significantly increased the interaction of mTOR and PPARg. In summary, PPARg plays an important role in the regulation of IGF-1 secretion and gene expression in response to dietary protein. PMID: 28257428
  4. Data suggest that both ovarian follicular dominance in cows and cooperation of ovarian follicles in pigs can be mediated by either down- or up-regulation of the insulin-like growth factor 1/oxytocin system. PMID: 28163019
  5. This study demonstrated that Up-regulation of IGF-1 in the frontal cortex of piglets exposed to an environmentally enriched arena. PMID: 28238777
  6. Combined administration of mesenchymal stem cells overexpressing IGF-1 and HGF enhances neovascularization but moderately improves cardiac regeneration in a porcine model. PMID: 27423905
  7. Results show that expression changes of IGF1 genes were associated with C/EBP b expression during embryonic and postnatal development in porcine liver. PMID: 25543525
  8. genetic association study suggests that the TC genotype of IGF-1 represents the selection genotype for smaller in size. PMID: 24634183
  9. IGF-I coordinately regulates multiple cell signaling pathways that are critical to proliferation, migration and survival of trophectoderm cells during early pregnancy in pigs. PMID: 24508636
  10. IGF-1 splice variant (mechano growth factor) is expressed within the growth plate, but the addition of its peptides was not associated with growth plate chondrocytes proliferation. PMID: 24146828
  11. alpha-linolenic acid increased IGF-I secretion from hepatocytes. PMID: 23053988
  12. Copper can stimulate chondrocyte proliferation by promoting the expression of IGF-1. PMID: 22367704
  13. The results show that modulation of plasma insulin levels by dietary carbohydrates seems possible in anabolic sows, but IGF-I levels are less easily modified. PMID: 21635573
  14. The results suggested that IFG-1 and -2 and their receptors are differentially expressed at the maternal and fetal components of the attachment site. PMID: 21685713
  15. Plasma ghrelin level and myocardial IGF-1 mRNA expression were significantly up-regulated, while plasma IGF-1 level and myocardial ghrelin mRNA expression were down-regulated in the chronic cyanotic immature piglets. PMID: 21933568
  16. IGFI signaling pathway regulates myofibre hypertrophy postnatally via complicated signal effectors, which may have negative impact on meat quality simultaneously. PMID: 21246287
  17. IGF-I, IGF-II, and IGFBP-3 mRNA were positively correlated with IGF-IR from 50E to 180D, suggesting that the expression of IGF-system genes exhibits specific developmental patterns in the skeletal muscle tissues. PMID: 21570979
  18. These results indicate that sorbic acid improves growth performance by regulating IGF system gene expression and hormone secretion. PMID: 21421836
  19. In an animal model of acute myocardial infarction relevant to human disease, intracoronary administration of IGF-1/hepatocyte growth factor (HGF) is a practical and effective strategy to reduce pathological cardiac remodeling. PMID: 21723061
  20. The expression of IGF-I and IGF-II in native growth plates of prepubertal piglets and under different cell culture conditions, was compared. PMID: 20600587
  21. The aims of this study were to investigate whether aldosterone influences IGF-I signaling and to determine the mechanism(s) by which aldosterone affects IGF-I function. PMID: 20881255
  22. Data show that hepatocytes secreted IGF-I under basal conditions and this was enhanced by GH addition. PMID: 19786114
  23. an important role of IGF1 in leptin receptor expression during the entire estrous cycle, with a special role during the prepubertal period PMID: 17139134
  24. study concludes that IGF1 promotes the FSH-stimulated synthesis and retention of hyaluronic acid in pig oocyte-cumulus complexes by PIK3/AKT- and MAPK3/1-dependent mechanisms PMID: 17329596
  25. Significant effect was found for IGF1 genotype on birth weight (P<0.05), with positive effects associated with the 286/286 genotype, and 280/286 genotype on litter weight at birth in second parity. PMID: 17369178
  26. Both classes of IGF-I mRNA were expressed in a variety of tissues, however, class 1 IGF-I mRNA was more abundant than class 2 in all tissues. PMID: 18157703
  27. in VSMCs exposed to hyperglycemia, IGF-I stimulation of Shc facilitates the transfer of Grb2 to p85 resulting in enhanced PI3K activation and AKT phosphorylation leading to enhanced cell proliferation and migration PMID: 18420583
  28. These data demonstrated that feed allowance level has important consequence on embryo survival, embryo development and uterine development, possibly mediated by changes in concentrations of reproductive hormones. PMID: 18700853

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Subcellular Location Secreted
Protein Families Insulin family
Database Links

KEGG: ssc:397491

STRING: 9823.ENSSSCP00000000915

UniGene: Ssc.16231

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