The product CSB-E04582r is a ready-to-use microwell, strip plate ELISA Kit for quantitative analysis of rat insulin-like growth factor 1 (IGF-1) in biological samples, including serum, plasma, cell culture supernates, or tissue homogenates. IGF-1, also called somatomedin C, is a hormone similar to insulin in molecular structure. IGF-1 exerts pleiotropic roles in various biological processes, including fetal development, growth during childhood and adolescence, and adult tissue homeostasis. Besides, IGF-1 seems to have atheroprotective actions, neural protective and reparative, and insulin-like effects and to regulate skeletal metabolism and muscle regeneration. However, IGF-1 has been demonstrated to regulate the cell cycle, apoptosis, cell proliferation, and survival in several tumors.
This kit has undergone rigorous quality tests in multiple parameters, including specificity, sensitivity, precision, linearity, recovery, and lot-to-lot consistency. The detection mechanism of this kit is based on the Sandwich-ELISA technique. IGF-1 in the samples or standards are sandwiched between pre-coated IGF-1 antibody and Biotin-conjugated IGF-1 antibody. The IGF-1/pre-coated IGF-1 antibody/Biotin-conjugated IGF-1 antibody complex is labeled by HRP and then occurs a chromogenic reaction after the addition of the TMB solution. The color intensity is positively related to the IGF-1 in the samples.