Rat Insulin-like growth factor 1,IGF-1 ELISA Kit

Code CSB-E04582r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
insulin-like growth factor 1 (somatomedin C)
Alternative Names
Igf1 ELISA Kit; Igf-1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
Uniprot No.
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
0.151 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.


To assess the linearity of the assay, samples were spiked with high concentrations of rat IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Materials provided
  • A micro ELISA plate --The 96-well plate has been pre-coated with an anti-rat IGF-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled IGF-1 antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
  • One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
  • One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Rat IGF1 ELISA Kit was designed for the quantitative measurement of Rat IGF1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.151 ng/mL.


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Target Background

(From Uniprot)
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
Gene References into Functions
  1. Study shows that insulin-like growth factor-1 potentiates sensory innervation signaling by modulating the mitochondrial fission/fusion balance. PMID: 28276453
  2. The article data suggest that 14 days under a 16:8 light-dark extended sleep photoperiod respectively down- and upregulated cortical adenosine AR and IGF-I levels in rats. PMID: 29149028
  3. suckling induces IGF-1 release either by elevating prolactin or growth hormone PMID: 29031906
  4. The endogenous growth hormone and IGF-1 were involved in the therapeutic effect of exogenous ghrelin in the healing of gingival mucosa damage. PMID: 29151078
  5. IgA enhances the IGF-1 activity in Glomerular Mesangial Cells via stimulation of IGF-1receptor gene transcription and suggest a role for IGF-1 in pathogenesis of IgA-Induced Nephropathy. PMID: 28668953
  6. NGF protects GECs against IND-induced injury. Mitochondria are major targets of both INDO-induced injury and NGF afforded protection of GECs. TrkA expression in the mitochondria of GECs indicates that the protection afforded by NGF is partly mediated by its direct action on mitochondria. NGF prevents MMP depolarization and increases expression of IGF-1 protein in GECs. PMID: 28843696
  7. study revealed that only BMP-6 was able to induce bone formation at the used dose and that the addition of IGF-1 contributed to an increase of the mineralization in the implants. Hence, the combination of BMP-6 with IGF-1 might be a better alternative than BMP-2 for orthopedic surgery or bone tissue engineering approaches. PMID: 28256809
  8. glutamate, by blocking IGF-1-mediated neuroprotection, could cause the apoptosis of retinal ganglion cells in the hypoxic neonatal retina. PMID: 27180072
  9. Following exercise, IGF-1 expression is increased in hippocampus and prefrontal cortex. PMID: 28179125
  10. Results indicate that a history of early adversity can evoke persistent changes in circulating insulin-like growth factor-1 and muscle mitochondrial function and content, which could serve to enhance predisposition for metabolic dysfunction in adulthood. PMID: 27196416
  11. The study findings show that IGF-1 and the local renin-angiotensin system interact to inhibit or activate neuroinflammation (i.e. transition from the M1 to the M2 phenotype), oxidative stress and dopaminergic degeneration. PMID: 27167199
  12. Self-Assembled Heterojunction Carbon Nanotubes Synergizing with Photoimmobilized IGF-1 Inhibit Cellular Senescence. PMID: 27385628
  13. Enhanced study of the role of IIS pathway and epigenetic mechanisms that regulate aging may facilitate progressive prevention and treatment of human age-related diseases. PMID: 28101820
  14. Collectively, the results indicate that androgen and IGF-I mutually interact and accelerate progesterone production, at least in part, by regulating endogenous BMP signaling in rat granulosa cells. PMID: 28684382
  15. Early IGF-1 primes visual cortex maturation and accelerates developmental switch between NKCC1 and KCC2 chloride transporters in enriched animals. Early enrichment and IGF-1 administration produced a decrease in the ratio between NKCC1 and KCC2 cation/chloride transporters, suggesting that enrichment, via IGF-1, may hasten the developmental switch of neuronal GABAergic responses from excitation to inhibition. PMID: 26924708
  16. Our study suggests that miR-29 downregulation, through its inverse regulation on downstream target of IGF1 gene, is a pro-angiogenic factor in MMEVC in type 2 diabetic rats. PMID: 28315330
  17. Data suggest Igf1 participates in neuroprotection of dorsal root ganglion neurons against toxic effects of environmental pollutants such as flame retardant BDE-209, a halogenated diphenyl ether; Igf1 promotes neurite outgrowth/cell viability and inhibits oxidative stress/apoptosis; Igf1 regulates expression of GAP-43 (growth-associated protein 43) and CGRP (calcitonin gene-related peptide) in opposition to BDE-209. PMID: 27090441
  18. IGF-1 activates ERalpha in ligand-independent manner in the hippocampus via phosphorylation at S118 resulting in increased association of ERalpha with steroid receptor coactivator 1 and elevation of ER-regulated proteins. PMID: 27254005
  19. Insulin growth factor binding protein 5 (IGFBP-5), insulin growth factor (IGF-1) and transforming growth factor (TGF-beta1) in the type II alveolar epithelial cells (AECs) play a key role in lung injury caused by bleomycin and pioglitazone attenuates the lung injury/fibrosis by restoring IGFBP-5 and IGF-1 and decreasing TGF-beta1 expressions in the type II AECs. PMID: 28077319
  20. Pappa has been shown to positively regulate the IGF signaling pathway, which is required for proper mammary gland/breast development and is of increasing interest in breast cancer pathogenesis. Mcs5c-mediated regulation of Pappa appears to occur through age-dependent and mammary gland-specific chromatin looping, as well as genotype-dependent CpG island shore methylation PMID: 27537370
  21. Data suggest that Igf1 (insulin-like growth factor-1) plays role in neuroprotection of dorsal root ganglion (DRG) neurons; Igf1 improves neuronal cell viability and promotes neurite growth via neuron-specific mechanisms (sensory neurons vs. neurofilament neurons). Here, Igf1 is involved in attenuation of apoptosis of DRG neurons in an in vitro model of microtubule modulator- (paclitaxel-)induced neurotoxicity. PMID: 25136768
  22. This study suggests that an obvious decrease in cavernous IGF-1 levels might play an important role in spontaneously hypertensive rats with erectile dysfunction. PMID: 26762757
  23. Data show that endogenous interleukins IL-27 and IL-35 (Ebi3 and Il-12p35 subunits) had inflammatory roles and insulin like growth factor -1 (IGF-1) had no effect. PMID: 26994309
  24. This study demonstrated that the Glucose metabolism and hepatic Igf1 DNA methylation are altered in the offspring of dams fed a low-salt diet during pregnancy. PMID: 26596702
  25. Salidroside hase cardioprotective effects in rats subjected to LPS-induced sepsis, probably through regulation of IGF-1/PI3K/Akt/GSK-3beta signaling. PMID: 26104971
  26. physiological levels of GH rapidly and potently activate Igf1 gene transcription while stimulating physical interactions in chromatin between inducible Stat5b-binding elements and the Igf1 promoters PMID: 26330488
  27. Prenatal ethanol exposure enhances the susceptibility to osteoarthritis in female adult rat offspring by down-regulating IGF-1 signaling and retarding articular cartilage development. PMID: 26434683
  28. Data suggest FSH and IGF1 (insulin-like growth factor-1) PI3K signaling pathways in granulosa cells converge at IRS1 (insulin receptor substrate 1), requiring PKA (cyclic AMP-dependent protein kinase) and PP1 (protein phosphatase 1) to regulate IRS1. PMID: 26702053
  29. PNE increases the susceptibility of adult offspring to OA; the potential mechanism involves IGF1 low-functional programming in articular cartilage caused directly by the action of nicotine on alpha4beta2-nAChR PMID: 26499267
  30. Downregulation of IGF1 by the introduction of miR-1 attenuated the proliferation of the vascular smooth muscle cells, suggesting that IGF1 is a target gene of miR-1 and that the effects of miR-1 are mediated through IGF1. PMID: 26166810
  31. data clearly indicate that IGF-1 plays a key-role in cortical plastic mechanisms and in behavioral alterations induced by a decrease in sensorimotor activity. PMID: 25958232
  32. Resveratrol reduced Igf1 levels in rat model of polycystic ovary syndrome. PMID: 25667201
  33. Findings suggest the possibility that insulin-like growth factor-1 signalling may be one of the underlying mechanisms involved in the beneficial effects of enriched environment in optimizing recovery following cerebral ischaemic injury. PMID: 24750178
  34. The IGF-1 inhibits the DM-induced colonic SMC apoptosis and might be involved in the alleviation of colonic dysmotility in diabetic rats. PMID: 25450576
  35. IGF1 and IGF2 are regulated in an organ-specific way in diabetic rats PMID: 25268143
  36. Results indicate that increased IGF-1 levels after recurrent hippocampal neuronal firings might, in turn, promote seizure activity via IGF-1R-dependent mechanisms. PMID: 26286172
  37. DHT suppresses the PGE2 and TGF-beta induced IGF-I gene promoter and differentiates other aspects of TGF-beta activity in osteoblasts PMID: 26187065
  38. Nutritional restoration after weaning (post liver differentiation) in maternal protein restriction rat offspring fails to prevent long-term impaired growth, in part, due to miR-29 suppression of hepatic Igf1 expression. PMID: 26151354
  39. study reports on a novel functional crosstalk between the IGF-1 and MSTN signaling pathways, as mediated through interaction between PI3K/Akt and Smad3 PMID: 26151859
  40. IGF-1 transgene facilitated the differentiation of spinal cord-derived neural stem cells into oligodendrocytes intended for spinal cord injury via the ERK1/2 pathway. PMID: 25162639
  41. Transplantation of BMSCs transfected with IGF-1 gene can promote fracture healing of rats with diabetes PMID: 25666965
  42. These results suggest that IGF-1 might contribute to cortical and striatal plasticity induced by a chronic sensorimotor restriction. PMID: 25226394
  43. Developmental and IUGR-induced DNA methylation occurred in a growth hormone response element site on the IGF-1 gene, CpG site-, and sex-specific manner. PMID: 25466885
  44. Lactoferrin (10 and 100 mug/mL) effectively inhibits apoptosis of primary rat osteoblasts by upregulating IGF-I expression. PMID: 24562308
  45. Our findings suggest that IGF-1 inhibits cells apoptosis in PASMC by activating the p38 MAPK-iNOS transduction pathway. PMID: 24673524
  46. reduced insulin secretion during protein restriction directly increased hepatic IRS-2 as a rapid response on day 1, while additional mechanisms contributed to the upregulation of IRS-2 on day 7 PMID: 25036495
  47. igf1 serum concentration fell, with a modification of GH daily pattern after mealtime PMID: 24617825
  48. MGF-Ct24E has a marked ability to increase bone formation by increasing cell proliferation and delaying cell differentiation during prophase, as well as by stimulating osteoblast differentiation during the advanced stage PMID: 24033831
  49. Circulating IGF-1 and IGFBP3 levels and hepatic mRNA expression of IGFBP1 and IGFBP2 were significantly decreased in maternal undernutrition and high fat offspring PMID: 23963811
  50. Endothelial insulin-like growth factor-1 modulates proliferation and phenotype of smooth muscle cells induced by low shear stress. PMID: 24322591

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Subcellular Location
Protein Families
Insulin family
Database Links
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