| Code |
CSB-E08055r |
| See More Details |
 |
| Product Type |
ELISA Kit |
| Size |
96T,5×96T,10×96T |
| Uniprot No. |
Q63264
|
| Lead Time |
3-5 working days |
| Abbreviation |
IL1B |
| Protein Biological Process 1 |
Immunity |
| Target Name |
interleukin 1, beta |
| Alias |
IL-1, IL1-BETA, IL1F2, catabolin|preinterleukin 1 beta|pro-interleukin-1-beta |
| Species |
Rattus norvegicus (Rat) |
| Protein Biological Process 3 |
Inflammatory response |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
62.5 pg/mL-4000 pg/mL |
| Sensitivity |
15.6 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Protocol |
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Target Details |
This protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. |
| HGNC |
5992 |
| RGD |
2891 |
| MGI |
96543 |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
98 |
|
| Range % |
95-103 |
|
| 1:2 |
Average % |
95 |
|
| Range % |
90-100 |
|
| 1:4 |
Average % |
97 |
|
| Range % |
95-103 |
|
| 1:8 |
Average % |
89 |
|
| Range % |
84-96 |
|
|
| Recovery |
| The recovery of rat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
88-96 |
|
| EDTA plasma (n=4) |
90 |
87-95 |
|
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 4000 |
2.475 |
2.459 |
2.467 |
2.287 |
|
| 2000 |
2.132 |
2.122 |
2.127 |
1.947 |
|
| 1000 |
1.557 |
1.543 |
1.550 |
1.370 |
|
| 500 |
0.912 |
0.905 |
0.909 |
0.729 |
|
| 250 |
0.582 |
0.574 |
0.578 |
0.398 |
|
| 125 |
0.397 |
0.388 |
0.393 |
0.213 |
|
| 62.5 |
0.275 |
0.267 |
0.271 |
0.091 |
|
| 0 |
0.180 |
0.179 |
0.180 |
|
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|
| References |
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Glycogen synthase kinase-3 beta inhibitors protectagainst the acute lung injuries resulting from acute necrotizing pancreatitis Jin H, et al,Acta Cirurgica Brasileira,2019
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Renal and testicular up-regulation of pro-inflammatory chemokines (RANTES and CCL2) and cytokines (TNF-α, IL-1β, IL-6) following acute edible camphor administration is through activation of NF-kB in rats Oluwatobi T.Somade, et al,Toxicology Reports,2019
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Systems Pharmacology and Microbiome Dissection of Shen Ling Bai Zhu San Reveal Multiscale Treatment Strategy for IBD Wei-jie Lv, et al,Journal of Physics: Conference Series,2019
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Melatonin improves functional recovery in female rats after acute spinal cord injury by modulating polarization of spinal microglial/macrophages Zhang Y,Journal of Neuroscience Research,2019
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6-Gingerol abates benzo[a]pyrene-induced colonic injury via suppression of oxido-inflammatory stress responses in BALB/c mice Ajayi BO, et al,Chemico-Biological Interactions,2019
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Anti-inflammatory Action of YHQ by Regulating 5-LOX/COX-2/NF-κB/MAPKs/Akt Signaling Pathways in RAW 264.7 Macrophage cells Hu TY, et al,Journal of Herbal Medicine,2019
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TLR4 participates in sympathetic hyperactivity Post-MI in the PVN by regulating NF-κB pathway and ROS production YuWang, et al,Redox Biology,2019
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The protective effect of kaempferol on heart via the regulation of Nrf2, NF-κβ, and PI3K/Akt/GSK-3β signaling pathways in isoproterenol-induced heart failure in diabetic rats Zhang L, et al,Drug development research,2019
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Protective effect of extract of Bletilla striata on isoflurane induced neuronal injury by altering PI3K/Akt pathway Guoqing Zhao, et al,Translational Neuroscience,2018
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Chamomile oil loaded solid lipid nanoparticles: A naturally formulated remedy to enhance the wound healing Heba A.Gad, et al,Journal of Drug Delivery Science and Technology,2019
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In vivo evaluation of chitosan based local delivery systems for atorvastatin in treatment of periodontitis A. Işılay Özdoğan.et al,International Journal of Pharmaceutics,2018
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Activation of Nrf2 signaling by salvianolic acid C attenuates NF‑κB mediated inflammatory response both in vivo and in vitro Junke Song, .et al,International Immunopharmacology,2018
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MicroRNA-494 inhibition alleviates acute lung injury through Nrf2 signaling pathway via NQO1 in sepsis-associated acute respiratory distress syndrome Yun Ling .et al,Life Sciences,2018
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Imbalanced oxidative stress and pro-inflammatory markers differentiate the development of diabetic neuropathy variants in streptozotocin-induced diabetic rats Che Aishah Nazariah.et al,Journal of Diabetes & Metabolic Disorders,2018
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Effect of Edible Bird’s Nest Extract on Lipopolysaccharide-Induced Impairment of Learning and Memory in Wistar Rats S. Careena.et al,evidence-based complementary and alternative medicine,2018
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Vinpocetine halts ketamine-induced schizophrenia-like deficits in rats: impact on BDNF and GSK-3β/β-catenin pathway Hebatalla I. Ahmed.et al,naunyn-schmiedebergs archives of pharmacology,2018
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Cognitive impairments induced by severe acute pancreatitis are attenuated by berberine treatment in rats Xiaofeng Ou.et al,Molecular MedicineReports,2018
-
Agmatine attenuates rhabdomyolysis-induced acute kidney injury in rats in a dose dependent manner Maha H.Sharawy.et al,Life Sciences,2018
-
Genipin attenuates hyperoxia-induced lung injury and pulmonary hypertension via targeting glycogen synthase kinase-3β in neonatal rats JingLi.et al,Nutrition,2018
-
Jatrorrhizine Hydrochloride Suppresses Proliferation, Migration, and Secretion of Synoviocytes In Vitro and Ameliorates Rat Models of Rheumatoid Arthritis In Vivo Haiwen Qiu.et al,Int. J. Mol. Sci.,2018
-
Inhibitory effects of tubeimoside I on synoviocytes and collagen‐induced arthritis in rats Zhenzhou Liu.et al,J CELL PHYSIOL,2018
-
The protective effects of selenium supplementation on ambient PM2.5-induced cardiovascular injury in rats Xuejiao Zeng.et al,Environmental Science and Pollution Research,2018
-
Ethanolamine enhances intestinal functions by altering gut microbiome and mucosal anti-stress capacity in weaned rats Jian Zhou.et al,British Journal of Nutrition,2018
-
Evidence that ADAM17 mediates the protective action of CGRP against angiotensin II-induced inflammation in vascular smooth muscle cells Si-yu Zeng.et al,/,/
-
Carnosic acid inhibits inflammation response and joint destruction on osteoclasts, fibroblast-like synoviocytes, and collagen-induced arthritis rats Liu M.et al,J Cell Physiol,2018
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Inhibition of nuclear factor kappa B pathway protects myocardial ischemia/reperfusion injury in rats under treatment with abnormal savda munziq
Maimaitiaili, Abudunaibi.et al,Am J Transl Res,2018
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-
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-
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Hypothermia followed by rapid rewarming exacerbates ischemia-induced brain injury andAugments inflammatory response in rats. Zhu SZ.et al,Biochem Biophys Res Commun.,2016
-
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