| Code |
CSB-E08055r |
| See More Details |
 |
| Product Type |
ELISA Kit |
| Size |
96T,5×96T,10×96T |
| Uniprot No. |
Q63264
|
| Lead Time |
3-5 working days |
| Abbreviation |
IL1B |
| Protein Biological Process 1 |
Immunity |
| Target Name |
interleukin 1, beta |
| Alias |
IL-1, IL1-BETA, IL1F2, catabolin|preinterleukin 1 beta|pro-interleukin-1-beta |
| Species |
Rattus norvegicus (Rat) |
| Protein Biological Process 3 |
Inflammatory response |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
62.5 pg/mL-4000 pg/mL |
| Sensitivity |
15.6 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Protocol |
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
|
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Target Details |
This protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. |
| HGNC |
5992 |
| RGD |
2891 |
| MGI |
96543 |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
98 |
|
| Range % |
95-103 |
|
| 1:2 |
Average % |
95 |
|
| Range % |
90-100 |
|
| 1:4 |
Average % |
97 |
|
| Range % |
95-103 |
|
| 1:8 |
Average % |
89 |
|
| Range % |
84-96 |
|
|
| Recovery |
| The recovery of rat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
88-96 |
|
| EDTA plasma (n=4) |
90 |
87-95 |
|
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 4000 |
2.475 |
2.459 |
2.467 |
2.287 |
|
| 2000 |
2.132 |
2.122 |
2.127 |
1.947 |
|
| 1000 |
1.557 |
1.543 |
1.550 |
1.370 |
|
| 500 |
0.912 |
0.905 |
0.909 |
0.729 |
|
| 250 |
0.582 |
0.574 |
0.578 |
0.398 |
|
| 125 |
0.397 |
0.388 |
0.393 |
0.213 |
|
| 62.5 |
0.275 |
0.267 |
0.271 |
0.091 |
|
| 0 |
0.180 |
0.179 |
0.180 |
|
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|
| References |
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Intranasal Chromium Induces Acute Brain and Lung Injuries in Rats: Assessment of Different Potential Hazardous Effects of Environmental and Occupational Abeer Salama.et al,PLoS ONE,Dec 20
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Total saponin of Dioscoreae hypoglaucae rhizoma ameliorates streptozotocin-induced diabetic nephropathy Guo C.et al,Drug Des Devel Ther.,2016 10:799-810.
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Paeoniflorin and Albiflorin Attenuate Neuropathic Pain via MAPK Pathway in Chronic Constriction Injury Rats Jianyu Zhou.et al,Evidence-Based Complementary and Alternative Medicine,/
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Investigation of selenium pretreatment in the attenuation of lung injury in rats induced by fine particulate matters Jie Liu.et al,Environ Sci Pollut Res ,Dec 5
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Neurolytic celiac plexus block enhances skeletal muscle insulin signaling and attenuates insulin resistance in GK rats Jun Li.et al,Experimental and Therapeutic Medicine,2016
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Rutin protects against aging-related metabolic dysfunction Li T. et al,Food Funct,2016 Jan 25.
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Protective Effects of Celastrol on Diabetic Liver Injury via TLR4/MyD88/NF-kB Signaling Pathway in Type 2 Diabetic Rats Li-ping Han. et al,Journal of Diabetes Research,2016 10pages
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5-lipoxygenase activation is involved in the mechanisms of chronic hepatic injury in a rat model of chronic aluminum overload exposure Mai S.et al,Toxicol Appl Pharmacol.,305:259-66
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Anti Qin Wang.et al,Experimental and therapeutic medicine 13(1),December
-
Critical role of regulator of calcineurin 1 in spinal cord injury. Wang G.et al,J Physiol Biochem.,Jan 9
-
“Three Methods and Three Points” regulates p38 mitogen-activated protein kinase in the dorsal horn of the spinal cord in a rat model of sciatic nerve injury Xin Guo.et al,Neural Regeneration Research,January
-
Xuefu ZhuyuDecoction, a traditional Chinese medicine, provides neuroprotection in a rat model of traumatic brain injury via an anti-inflammatory pathway Xing Z.et al,Sci Rep.,2016 6:20040.
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Effects of Castanospermine on Inflammatory Response in a Rat Model of Experimental Severe Acute Pancreatitis Yu-pu Hong.et al,Archives of Medical Research,August
-
Hypothermia followed by rapid rewarming exacerbates ischemia-induced brain injury andAugments inflammatory response in rats. Zhu SZ.et al,Biochem Biophys Res Commun.,474(1):175-81
-
Therapeutic effect of Lianbeijuqin (a Chinese herbal cocktail) on periodontitis in rat Jie Zhang.et al,Tropical Journal of Pharmaceutical Research ,Feb 15
-
Protective effect of coptisine free base on indomethacin-induced gastric ulcers in rats: Characterization of potential molecular mechanisms Luo C.et al,Life Sci,Dec 6
-
Involvement of S100A8/A9-TLR4-NLRP3 Inflammasome Pathway in Contrast-Induced Acute Kidney Injury Tan X.et al,Cell Physiol Biochem.,Aug 30
-
Acupuncture alters pro-inflammatory cytokines in the plasma of maternallySeparated rat pups Wang Z.et al,Chin J Integr Med. ,Oct 6
-
Ulinastatin ameliorates tissue damage of severe acute pancreatitis through modulating regulatory T cells Yu Pan.et al,Journal of Inflammation,Mar 20
-
Paeoniflorin attenuates the neuroinflammatory response in a rat model of chronic constriction injury Zhou J.et al,Mol Med Rep. ,Mar 24.
-
Inhibition of nuclear factor kappa B pathway protects myocardial ischemia/reperfusion injury in rats under treatment with abnormal savda munziq
Maimaitiaili, Abudunaibi.et al,Am J Transl Res,/
-
Minocycline and Ifenprodil Prevent Development of Painful Diabetic Neuropathy in Streptozotocin-induced Diabetic Rat Model Che Aishah Nazariah Ismail.et al,Life Sciences,Feb 24
-
Carnosic acid inhibits inflammation response and joint destruction on osteoclasts, fibroblast-like synoviocytes, and collagen-induced arthritis rats Liu M.et al,J Cell Physiol,Mar 9
-
Evidence that ADAM17 mediates the protective action of CGRP against angiotensin II-induced inflammation in vascular smooth muscle cells Si-yu Zeng.et al,/,/
-
Ethanolamine enhances intestinal functions by altering gut microbiome and mucosal anti-stress capacity in weaned rats Jian Zhou.et al,British Journal of Nutrition,May 23
-
The protective effects of selenium supplementation on ambient PM2.5-induced cardiovascular injury in rats Xuejiao Zeng.et al,Environmental Science and Pollution Research,May 26
-
Inhibitory effects of tubeimoside I on synoviocytes and collagen‐induced arthritis in rats Zhenzhou Liu.et al,J CELL PHYSIOL,May 15
-
Jatrorrhizine Hydrochloride Suppresses Proliferation, Migration, and Secretion of Synoviocytes In Vitro and Ameliorates Rat Models of Rheumatoid Arthritis In Vivo Haiwen Qiu.et al,Int. J. Mol. Sci.,May 18
-
Genipin attenuates hyperoxia-induced lung injury and pulmonary hypertension via targeting glycogen synthase kinase-3β in neonatal rats JingLi.et al,Nutrition,June 19
-
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-
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-
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-
Imbalanced oxidative stress and pro-inflammatory markers differentiate the development of diabetic neuropathy variants in streptozotocin-induced diabetic rats Che Aishah Nazariah.et al,Journal of Diabetes & Metabolic Disorders,August 16
-
MicroRNA-494 inhibition alleviates acute lung injury through Nrf2 signaling pathway via NQO1 in sepsis-associated acute respiratory distress syndrome Yun Ling .et al,Life Sciences,August 17
-
Activation of Nrf2 signaling by salvianolic acid C attenuates NF‑κB mediated inflammatory response both in vivo and in vitro Junke Song, .et al,International Immunopharmacology,August 22
-
In vivo evaluation of chitosan based local delivery systems for atorvastatin in treatment of periodontitis A. Işılay Özdoğan.et al,International Journal of Pharmaceutics,September 5
-
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The protective effect of kaempferol on heart via the regulation of Nrf2, NF-κβ, and PI3K/Akt/GSK-3β signaling pathways in isoproterenol-induced heart failure in diabetic rats Zhang L, et al,Drug development research,March 12
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