Rat Interleukin 1β,IL-1β ELISA Kit

Instructions
Code CSB-E08055r
Size 96T,5×96T,10×96T

Product Details

Target Name interleukin 1, beta
Alternative Names IL-1, IL1-BETA, IL1F2, catabolin|preinterleukin 1 beta|pro-interleukin-1-beta
Abbreviation IL1B
Uniprot No. Q63264
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 95-103  
1:2 Average % 95  
Range % 90-100  
1:4 Average % 97  
Range % 95-103  
1:8 Average % 89  
Range % 84-96  
Recovery
The recovery of rat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-96  
EDTA plasma (n=4) 90 87-95  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.475 2.459 2.467 2.287  
2000 2.132 2.122 2.127 1.947  
1000 1.557 1.543 1.550 1.370  
500 0.912 0.905 0.909 0.729  
250 0.582 0.574 0.578 0.398  
125 0.397 0.388 0.393 0.213  
62.5 0.275 0.267 0.271 0.091  
0 0.180 0.179 0.180    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Q&A and Customer Reviews

 Customer Reviews
  • Tested application: ELISA
    Test sample: Tissue homogenate (brain)
    Species: Rat
    Review: The test is carried out in a simple way, the samples in repetitions come out very repetitive, only small differences in groups. The samples were not diluted, the results approximated to half the standard curve. The samples were converted to the amount of protein calculated using the BCA method.

Target Data

Function Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Subcellular Location Cytoplasm, cytosol, Lysosome, Secreted, exosome, Cytoplasmic vesicle, autophagosome, Secreted
Protein Families IL-1 family
Database Links

STRING: 10116.ENSRNOP00000006308

UniGene: Rn.9869

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