Rat Interleukin 1β,IL-1β ELISA Kit

Code CSB-E08055r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

CUSABIO's IL1B rat ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL1B in serum, plasma, or tissue homogenates. It has good reproducibility, high specificity & sensitivity, and good linearity. This assay uses an antibody specific for rat IL1B coated on a 96-well plate. IL1B present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated IL1B antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1B bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader. This IL1B rat ELISA kit has been described in up to 150 publications.

IL1B is a potent pro-inflammatory cytokine essential for host-defense responses to infection and injury. IL1B binds to IL1RI and then associates with the IL-1RAcP, initiating the recruitment of the MyD88 adaptor and activation and ligation to IRAK4, activating the MAPK p38 cascade and the NF-κB signaling pathways that are implicated in inflammatory responses. It is involved in pain, inflammation, and autoimmune disorders. IL1B also exerts important homeostatic effects in the normal organism, including the modulation of temperature, feeding, and sleep. Aberrant expression of IL1B has been found in various fibrotic diseases. For example, IL1B overexpression participates in the pathophysiological changes that occur during different disease states, such as rheumatoid arthritis, osteoarthritis, and Alzheimer's disease.

Alternative Names Il1bInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
Abbreviation IL1B
Uniprot No. Q63264
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates.
Detection Range 0.156 ng/ml-10 ng/ml.
Sensitivity 0.039 ng/ml.
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
Subcellular Location Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
Protein Families IL-1 family
Database Links

STRING: 10116.ENSRNOP00000006308

UniGene: Rn.9869

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