Rat Interleukin 1β,IL-1β ELISA Kit

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Rat Interleukin 1β,IL-1β ELISA Kit

Name: Rat Interleukin 1β,IL-1β ELISA Kit
Brand: CUSABIO
SKU: CSB-E08055r

^Instructions

Code	CSB-E08055r
Size	96T,5×96T,10×96T
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Target Data

Target Name

interleukin 1, beta

Alternative Names

Il1bInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit

Abbreviation

IL1B

Uniprot No.

Q63264

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	95-103
1:2	Average %	95
	Range %	90-100
1:4	Average %	97
	Range %	95-103
1:8	Average %	89
	Range %	84-96

Recovery

The recovery of rat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	90	87-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.475	2.459	2.467	2.287
2000	2.132	2.122	2.127	1.947
1000	1.557	1.543	1.550	1.370
500	0.912	0.905	0.909	0.729
250	0.582	0.574	0.578	0.398
125	0.397	0.388	0.393	0.213
62.5	0.275	0.267	0.271	0.091
0	0.180	0.179	0.180

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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■ Customer Reviews

Tested application: ELISA
Test sample: Tissue homogenate (brain)
Species: Rat
Review: The test is carried out in a simple way, the samples in repetitions come out very repetitive, only small differences in groups. The samples were not diluted, the results approximated to half the standard curve. The samples were converted to the amount of protein calculated using the BCA method.

Function	Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Subcellular Location	Cytoplasm, cytosol, Lysosome, Secreted, exosome, Cytoplasmic vesicle, autophagosome, Secreted
Protein Families	IL-1 family
Database Links	STRING: 10116.ENSRNOP00000006308 UniGene: Rn.9869

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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