Rat Interleukin 1β,IL-1β ELISA Kit

Code CSB-E08055r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q63264
Lead Time 3-5 working days
Abbreviation IL1B
Protein Biological Process 1 Immunity
Target Name interleukin 1, beta
Alias IL-1, IL1-BETA, IL1F2, catabolin|preinterleukin 1 beta|pro-interleukin-1-beta
Species Rattus norvegicus (Rat)
Protein Biological Process 3 Inflammatory response
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
HGNC 5992
RGD 2891
MGI 96543
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 95-103  
1:2 Average % 95  
Range % 90-100  
1:4 Average % 97  
Range % 95-103  
1:8 Average % 89  
Range % 84-96  
Recovery
The recovery of rat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-96  
EDTA plasma (n=4) 90 87-95  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.475 2.459 2.467 2.287  
2000 2.132 2.122 2.127 1.947  
1000 1.557 1.543 1.550 1.370  
500 0.912 0.905 0.909 0.729  
250 0.582 0.574 0.578 0.398  
125 0.397 0.388 0.393 0.213  
62.5 0.275 0.267 0.271 0.091  
0 0.180 0.179 0.180    
References
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Function Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Subcellular Location Cytoplasm, cytosol, Lysosome, Secreted, exosome, Cytoplasmic vesicle, autophagosome, Secreted
Protein Families IL-1 family
Database Links

STRING: 10116.ENSRNOP00000006308

UniGene: Rn.9869

Pathway MAPK signaling pathway
Lipids and Inflammation in Atherogenesis
NF-kappa B signaling pathway
TNF signaling pathway
Necroptosis
Hematopoietic cell lineage
IL-17 signaling pathway
Inflammasome Signaling
NOD-like receptor signaling pathway
Osteoclast differentiation
Th17 cell differentiation
Toll-like receptor signaling pathway
AGE-RAGE signaling pathway in diabetic complications
Excitatory synapse pathway

Q&A and Customer Reviews

Customer Reviews
  • Tested application: ELISA
    Test sample: Tissue homogenate (brain)
    Species: Rat
    Review: The test is carried out in a simple way, the samples in repetitions come out very repetitive, only small differences in groups. The samples were not diluted, the results approximated to half the standard curve. The samples were converted to the amount of protein calculated using the BCA method.

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