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| Code | CSB-E04628r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-99 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| The recovery of rat IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-96 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The rat IL-2 ELISA kit is a solid-phase immunoassay specially designed to quantitatively measure rat IL-2 in serum, plasma, or tissue homogenates. It is based on the Sandwich-ELISA mechanism. IL-2 in the sample is bound to the capture antibody immobilized on the 96-well strip plate and then sandwiched with the biotinylated IL-2 antibody. After the addition of HRP-avidin and TMB substrate, the solution in the wells turns blue. The color reaction is stopped by adding the stop solution into the wells, and the color changes from blue to yellow. The color intensity is positively proportional to the IL-2 bound in the initial step. The IL-2 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.
IL-2 is a key immunoregulatory cytokine that promotes the activation, proliferation, and differentiation of CD4+ T helper subsets and CD4+ T regulatory cells. IL-2 attaches to its receptor, which is made up of three subunits IL-2Rα, IL-2Rβ, and γc, triggering the phosphorylation of transcription factors, activating JAK-STAT, PI3K-AKT, and MAPK pathways, leading to induction of gene expression. IL-2 activity has been found in activated T cell supernatants and has been shown to mediate human T cell expansion and proliferation. IL-2 has wide essential biological activities in driving T cell proliferation and regulating effector cell differentiation.
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