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Rat Interleukin 2,IL-2 ELISA kit

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Citations (13) Protocol MSDS
Code CSB-E04628r
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 2
Alternative Names
Il2 ELISA kit; Il-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
Abbreviation
IL2
Uniprot No.
P17108
Species
Rattus norvegicus (Rat)
Sample Types
serum, urine, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 92  
Range % 87-98  
1:2 Average % 98  
Range % 91-104  
1:4 Average % 89  
Range % 84-99  
1:8 Average % 96  
Range % 92-100  
Recovery
The recovery of rat IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 85-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.489 2.478 2.484 2.289  
200 1.954 1.896 1.925 1.730  
100 1.292 1.273 1.283 1.088  
50 0.797 0.768 0.783 0.588  
25 0.503 0.489 0.496 0.301  
12.5 0.364 0.356 0.360 0.165  
6.25 0.276 0.258 0.267 0.072  
0 0.198 0.192 0.195 　  
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

The rat IL-2 ELISA kit is a solid-phase immunoassay specially designed to quantitatively measure rat IL-2 in serum, plasma, or tissue homogenates. It is based on the Sandwich-ELISA mechanism. IL-2 in the sample is bound to the capture antibody immobilized on the 96-well strip plate and then sandwiched with the biotinylated IL-2 antibody. After the addition of HRP-avidin and TMB substrate, the solution in the wells turns blue. The color reaction is stopped by adding the stop solution into the wells, and the color changes from blue to yellow. The color intensity is positively proportional to the IL-2 bound in the initial step. The IL-2 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

IL-2 is a key immunoregulatory cytokine that promotes the activation, proliferation, and differentiation of CD4+ T helper subsets and CD4+ T regulatory cells. IL-2 attaches to its receptor, which is made up of three subunits IL-2Rα, IL-2Rβ, and γc, triggering the phosphorylation of transcription factors, activating JAK-STAT, PI3K-AKT, and MAPK pathways, leading to induction of gene expression. IL-2 activity has been found in activated T cell supernatants and has been shown to mediate human T cell expansion and proliferation. IL-2 has wide essential biological activities in driving T cell proliferation and regulating effector cell differentiation.

Citations

Related Products

Il2 Proteins

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Target Background

Function
(From Uniprot)
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Gene References into Functions
  1. Data indicate that C-rel binding on the interleukin-2 (IL-2) promoter region declined in the aged CD4+T cells. PMID: 24853588
  2. our findings indicate a generic and robust stress-induced reduction in plasma IL-12 PMID: 22659252
  3. Effects of systemic IL-2 on neurotransmission in several cortical areas is measured by in-vivo microdialysis; IL-2 has dose-dependent effects on depressive-related behavior after delayed but not acute testing, but no effects on anxiety-like behavior. PMID: 21726905
  4. The purpose of this study was to evaluate the effect of exhaustive exercise on proteins associated with muscle damage and regeneration, including IL-2, IL-4 and MyoD in muscle and adipose tissue. PMID: 21668966
  5. IL-2 neuroprotective action upon retinal ganglion cells in vitro is mediated by Janus kinase and extracellular signal-regulated kinase 1/2 activation. PMID: 21262542
  6. In a living cell imaging study, treatment with IL-2 at different concentrations during development of cultured hippocampal neurons induces major changes in dendritic branches, filopodia mobility, and spine density. PMID: 20225203
  7. The pIL-2-shRNA interference plasmid could decrease proinflammatory cytokine production of T lymphocytes and protect hepatocytes from apoptosis in allografts. PMID: 19589546
  8. IL-2 may participate in the immune rejection in allogeneic liver transplantation. PMID: 18471351
  9. effects of IL-2 on contraction and the [Ca2+]i transient of ventricular myocytes are mediated via the cardiac kappa opioid receptor. PMID: 11907831
  10. IL-1alpha and IL-2 produce an increase of glucose metabolism in uteri isolated from underfed rats PMID: 12878449
  11. Analysis of murine CD28 mutants reveals a correlation between translocation to lipid rafts and costimulation of IL-2 production. PMID: 15280538
  12. These results provide new evidence indicating that cytokine mRNA in the brain can be related to elevated plus-maze behavior and that this relationship is site (prefrontal cortex, striatum)- and cytokine mRNA-specific (IL-2). PMID: 15950774
  13. hypothalamic IL2 is decreased after intranasal administration of Epithalon PMID: 17152351
  14. stress-induced increase in serum IL-6 level and decrease in IL-2 level PMID: 17433709
  15. Cell activation in hypothalamus correlated with decreases in the quantity of IL-2-positive cells. PMID: 18264770
  16. Data suggest that estradiol effects on T cell IFN-gamma abd IL-2 production afer trauma-hemorrhage are mediated via non-genomic pathway, and which is in turn mediated via ERK, p38, and JNK pathways. PMID: 18343154
  17. Data show that inescapable and escapable stressors have different effects on behavior in the modified active avoidance test and on IL-2 levels in brain areas that are known to be involved in emotional processes. PMID: 18628674
  18. CD4+CD25+ T cells alloactivated ex vivo by IL-2 or IL-4 become potent alloantigen-specific inhibitors of rejection with different phenotypes, suggesting separate pathways of activation by Th1 and Th2 responses. PMID: 18827184

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Subcellular Location
Secreted.
Protein Families
IL-2 family
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Related pathways
Jak-STAT signaling pathway PI3K-Akt signaling pathway T cell receptor signaling pathway Th1 and Th2 cell differentiation Th17 cell differentiation
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