Rat growth-regulated oncogene α/melanoma growth stimulating activity,GROα /MGSA Elisa Kit

Code CSB-E12997r
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details


This Rat CXCL1 ELISA Kit was designed for the quantitative measurement of Rat CXCL1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

Target Name chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
Alternative Names Cxcl1 ELISA Kit; Cinc1 ELISA Kit; Gro ELISA Kit; Scyb1Growth-regulated alpha protein ELISA Kit; C-X-C motif chemokine 1 ELISA Kit; Cytokine-induced neutrophil chemoattractant 1 ELISA Kit; CINC-1 ELISA Kit; Platelet-derived growth factor-inducible protein KC ELISA Kit
Abbreviation CXCL1
Uniprot No. P14095
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat GROα /MGSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 86-95
1:2 Average % 106
Range % 101-112
1:4 Average % 93
Range % 87-96
1:8 Average % 93
Range % 86-98
The recovery of rat GROα /MGSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 89-98
EDTA plasma (n=4) 99 96-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
8000 2.454 2.589 2.522 2.396
4000 2.012 2.180 2.096 1.970
2000 1.445 1.496 1.471 1.345
1000 0.784 0.809 0.797 0.671
500 0.433 0.441 0.437 0.311
250 0.236 0.249 0.243 0.117
125 0.173 0.179 0.176 0.050
0 0.125 0.127 0.126  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

(From Uniprot)
Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation.
Gene References into Functions
  1. The results showed that after short-term incubation both CXCL1 and CXCL2 can activate TRPV1+/IB4+ DRG neurons by significantly reducing TRPV1 desensitization, while at acute application both chemokines induced an increase in [Ca2+]i although of different amplitudes, in a subset of neurons partially also itch-sensitive in the case of CXCL1, or non-itch sensitive in the case of CXCL2. PMID: 28820395
  2. remote ischemic preconditioning protects hepatic microcirculation by induction of HO and modulation of cytokine induced neutrophil chemoattractant in hepatic ischemia reperfusion injury PMID: 27672274
  3. Results results demonstrate that sphingosine-1-phosphate induces Ca(2+) signaling in astrocytes via Gq -coupled receptors S1P2 and S1P3 , followed by Ca(2+) influx through TRPC6 that could activate MAPK signaling, which leads to increased secretion of the proinflammatory or neuroprotective chemokine CXCL1 PMID: 28300348
  4. aging can reduce the expression of CINC and MCP-1 mRNA in lung tissues, and reduce the infiltration of neutrophils and monocyte-macrophages induced by CINC and MCP-1. This might lead to increased risk of pneumonia in elderly patients. PMID: 24464584
  5. In opioid-tolerant rats, CXCL1 is up-regulated in CSF; as it is in patients also. PMID: 25383568
  6. Function of the transient receptor potential vanilloid (TRPV)1 channel is enhanced by dorsal root ganglia inflammation. The effects (decreased tachyphylaxis) are mimicked by incubation with GRO/KC. PMID: 22466126
  7. CXCL1 inhibition of oligodendrocyte progenitor cell migration is regulated via changes in intracellular calcium flux. PMID: 22554866
  8. hyperoxia exposure led to a suppression of the HDAC1/HDAC2 expression and activity, and the overall HDAC activity, as well as arrest of alveolarization, and an elevated expression of CINC-1 in the lungs of newborn rats PMID: 21905265
  9. Advanced glycation end products promote the secretion of MCP-1 and IL-8 in vascular smooth muscle cells. PMID: 21418993
  10. Severe burn injury activates pulmonary NF-kappaB and leads to secretion of IL8 and TNF alpha in the lung. PMID: 17959522
  11. In rats with chronic nonbacterial prostatitis, the levels of TNF-alpha and IL-8 in the serum and prostate tissues were significantly lower in the curcumin-treated group than in the positive control group. PMID: 20180411
  12. ATP-stimulated C6 glioma cells showed enhanced expression of MCP-1 & IL-8; elevated levels of MCP-1 & IL-8 are predicted to enhance mobility of tumor cells & promote recruitment of microglia into developing tumors thereby supporting tumor growth. PMID: 20003523
  13. These results suggest that IL-6 and CINC-1 contribute to alpha2M production in rats only when IL-6 and CINC-1 act synergistically. PMID: 21030786
  14. The decreased serum concentration of CINC-1 as neuroprotective agent and increased CINC-2alpha in late stage of Morris hepatoma may be considered for their contribution to cerebellar degeneration. PMID: 20602290
  15. Rats treated with fullerenes showed a transient significant increase of neutrophils and expression of CINC-1,-2alphabeta and -3. PMID: 20226088
  16. CINC-1, released at sites of inflammation, mediates inflammatory hyperalgesia in rats via release of sympathomimetic amines. PMID: 12517731
  17. CINC-1 also functions as an early acute-phase protein after injury to the brain or to peripheral tissues PMID: 12709409
  18. neutrophil migration induced by IL-8 is dependent on CINC-1 release from mast cells PMID: 12824006
  19. Data show that TII alveolar epithelial cells produce three of the major proinflammatory CXC chemokines (GRO, CINC-2alpha, and MIP-2) and their cognate receptor CXCR2. PMID: 12829448
  20. CINC, in contrast to MIP-2, is selectively transported from the lung to the systemic circulation, where it promotes neutrophil migration into the lung in response to a chemotactic stimulus. PMID: 14617513
  21. reactive oxygen species and lipid peroxidation mainly derived from neutrophils, which are stimulated and mobilized by TNF-alpha and cytokine-induced neutrophil chemoattractant-1 PMID: 15942680
  22. CINC-1 may contribute to liver repair and regeneration PMID: 16271365
  23. In summary, hyperalgesia, local PGE2 production, and spinal c-Fos expression occur after CFA-induced inflammation but not after CXCL1- or CXCL2/3-induced, selective PMN recruitment. PMID: 16522746
  24. results demonstrate that ascorbic acid deficiency elevates the serum, liver and spleen concentrations of cytokine-induced neutrophil chemoattractant-1 (CINC-1 as seen in acute inflammation PMID: 16637227
  25. Following cecal ligation and puncture CINC-1 production increases in the lung, setting the stage for neutrophil accumulation in lung during sepsis. PMID: 16818791
  26. activation of PAR-2, as well as PAR-1, can rescue astrocytes from ceramide-induced apoptosis via regulating chemokine GRO/CINC-1 release. PMID: 16942465
  27. berberine dose-dependently inhibited the expression of CINC-1 induced by LPS and diminished the anterior uveitis. PMID: 17164575
  28. TNFalpha has in different forms of arthritis, but points out the idea that CINC-1 (the homologue for human IL-8) may constitute a promising target for reactive arthritis management PMID: 17166735
  29. basal level of calcium is the pre-requisite for GRO/CINC-1 protein synthesis and secretion PMID: 17666044
  30. Induction of CXCL1 by extracellular matrix and autocrine enhancement by interleukin-1 in rat pancreatic beta-cells. PMID: 17702850
  31. GGA effectively suppressed the up-regulation of GRO1 messenger ribonucleic acid, and this was validated by Northern hybridization. PMID: 18683011
  32. This is the first time that centrally injected CINC-1 has been reported to act directly on the pyrogen-sensitive neurons of the preoptic area, promoting a thermoregulatory response that seems to depend on other endogenous pyrogens synthesis and PGE2. PMID: 18694739
  33. CXCL1 incubation (1.5 nM, overnight) caused marked upregulation of Na+ currents in acutely isolated small diameter rat (adult) sensory neurons in vitro. PMID: 18816377
  34. The results suggest that GRO/KC has important effects in inflammatory processes via its direct actions on sensory neurons. PMID: 19476648
  35. CINC-1 is a neutrophil chemoattractant; findings have established that CINC-1 also functions as an early acute-phase protein after injury to the brain or to peripheral tissues. PMID: 12709409

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Subcellular Location Secreted.
Protein Families Intercrine alpha (chemokine CxC) family
Tissue Specificity At least expressed in the lung and trachea.
Database Links

KEGG: rno:81503

STRING: 10116.ENSRNOP00000003778

UniGene: Rn.10907

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