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Sheep insulin-like growth factor 1,IGF-1 ELISA Kit

Citations (1) Protocol
Code CSB-E13753Sh
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
insulin-like growth factor 1 (somatomedin C)
Alternative Names
IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
Abbreviation
IGF1
Uniprot No.
P10763
Species
Ovis aries (Sheep)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 ng/mL-1000 ng/mL
Sensitivity
3.9 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %86
Range %80-95
1:2Average %95
Range %87-101
1:4Average %97
Range %92-103
1:8Average %93
Range %82-98
Recovery
The recovery of sheep IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9087-92
EDTA plasma (n=4)9790-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
10002.842 2.795 2.819 2.657
5002.015 2.076 2.046 1.884
2501.137 1.161 1.149 0.987
1250.588 0.604 0.596 0.434
62.50.357 0.372 0.365 0.203
31.20.278 0.293 0.286 0.124
15.60.234 0.242 0.238 0.076
00.164 0.160 0.162
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Sheep IGF1 ELISA Kit was designed for the quantitative measurement of Sheep IGF1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

Citations

Related Products

IGF1 Proteins

See all products about IGF1 >>

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Target Background

Function
(From Uniprot)
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.
Gene References into Functions
  1. MO induced an increase in liver conversion of cortisone to cortisol in adult offspring and support a role for leptin signaling in the pituitary in mediating offspring adiposity PMID: 28771488
  2. This study described the distribution of inflammatory factors, especially insulin-like growth factor-1 and neutrophil gelatinase-associated lipocalin (NGAL). PMID: 25193468
  3. DLK-1 expression was significantly (P < 0.01) and positively correlated with IGF-I expression. PMID: 25501243
  4. Poor maternal nutrition during gestation reduced growth rate in offspring which may be because of reduced circulating IGF-I and IGFBP-3 and decreased expression of IGFBP-3 in the liver. PMID: 24996190
  5. The mRNA expression levels of MSTN, IGF-1 and IGF-2 were significantly different in the same skeletal muscle under different dietary intake. PMID: 25078581
  6. We conclude that the expressions of IGF-I and MyoG genes are significantly and positively correlated with early muscle traits of Hu sheep PMID: 24782091
  7. Data show that both short- and long-term exposure to high-altitude conditions decreased luteal growth and IGF-I and IGF-II gene expression but increased HIF-1 alpha and VEGF immunoexpression. PMID: 23521851
  8. Variation of the number of CA repeats in the microsatellite repeat sequence within the 5 prime flanking region of ovine IGF1 gene was identified. PMID: 22729916
  9. Report differential regulation of igf1 and igf1r mRNA levels in the two hepatic lobes following intrauterine growth restriction and its treatment with intra-amniotic insulin-like growth factor-1 in ovine fetuses. PMID: 20883644
  10. We determined the effect of insulin and/or recombinant human (rh)IGF-I infusion on ovine fetal phenylalanine kinetics, protein synthesis, and phenylalanine accretion PMID: 15644452
  11. results suggest that GH-R, IGF-1 and IGF-IR mRNA expression in the skin of sheep follows specific developmental patterns PMID: 16963415
  12. intrauterine overfeeding during early to mid gestation is associated with increases in fetal blood concentrations of cortisol and IGF-1, in association with ventricular hypertrophy where reduced expression of CNTFRalpha & STAT3 may play a role. PMID: 17869083
  13. hepatic expression of IGF1 decreased in placental restriction-hypoxic reduced liver growth PMID: 19208549

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Subcellular Location
Secreted.
Protein Families
Insulin family
Database Links

KEGG: oas:443318

UniGene: Oar.375

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