Phospho-CTNNB1 (Tyr333) Antibody

Datasheet
Code CSB-PA150703
Size US$360
Uniprot No. P35222
Image
  • Western blot analysis of extracts from 293 cells untreated or treated with FSK using b-catenin(phospho-Tyr333) Antibody.

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using β-catenin (phospho-Tyr333) Antibody.

Immunogen Peptide sequence around phosphorylation site of tyrosine 333 (Y-T-Y(p)-E-K) derived from Human β-catenin
Raised in Rabbit
Species Reactivity Human
Specificity The antibody detects endogenous level of β-catenin only when phosphorylated at tyrosine 333.
Tested Applications ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:100
Relevance

Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML.

Lillehoj E.P., Lu W., Kiser T., Goldblum S.E., Kim K.C.Biochim. Biophys. Acta 1773:1028-1038(2007)
Weiske J., Albring K.F., Huber O.Proc. Natl. Acad. Sci. U.S.A. 104:20344-20349(2007)
Bahmanyar S., Kaplan D.D., Deluca J.G., Giddings T.H. Jr., O'Toole E.T., Winey M., Salmon E.D., Casey P.J., Nelson W.J., Barth A.I.Genes Dev. 22:91-105(2008)
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias CTNNB1; CATNB; CTNB1; CTNNB;
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Target Names CTNNB1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin
Involvement in disease Colorectal cancer (CRC); Pilomatrixoma (PTR); Medulloblastoma (MDB); Ovarian cancer (OC); Mesothelioma, malignant (MESOM); Mental retardation, autosomal dominant 19 (MRD19); Vitreoretinopathy, exudative 7 (EVR7)
Subcellular Location Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, Cell junction, adherens junction, Cell junction, Cell membrane, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole, Cell junction, synapse
Protein Families Beta-catenin family
Tissue Specificity Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). Expressed in breast cancer tissues (at protein level) (Pub
Database Links

HGNC: 2514

OMIM: 114500

KEGG: hsa:1499

STRING: 9606.ENSP00000344456

UniGene: Hs.476018

Pathway Hippo signaling pathway
Melanogenesis
Wnt signaling pathway
Focal adhesion
Adherens junction
Signaling pathways regulating pluripotency of stem cells
Leukocyte transendothelial migration
Rap1 signaling pathway

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