Recombinant Mouse Dual specificity mitogen-activated protein kinase kinase 2(Map2k2)

Code CSB-YP723614MO
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Source Yeast
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Code CSB-EP723614MO
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Source E.coli
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Code CSB-EP723614MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP723614MO
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Source Baculovirus
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Code CSB-MP723614MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Map2k2
Uniprot No. Q63932
Alternative Names Map2k2; Mek2; Mkk2; Prkmk2; Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; EC 2.7.12.2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2
Species Mus musculus (Mouse)
Expression Region 1-401
Target Protein Sequence MLARRKPVLP ALTINPTIAE GPSPTSEGAS EANLVDLQKK LEELDLDEQQ RKRLEAFLTQ KAKVGELKDD DFERISELGA GNGGVVTKAR HRPSGLIMAR KLIHLEIKPA VRNQIIRELQ VLHECNSPYI VGFYGAFYSD GEISICMEHM DGGSLDQVLK EAKRIPEDIL GKVSIAVLRG LAYLREKHQI MHRDVKPSNI LVNSRGEIKL CDFGVSGQLI DSMANSFVGT RSYMSPERLQ GTHYSVQSDI WSMGLSLVEL AIGRYPIPPP DAKELEASFG RPVVDGADGE PHSVSPRPRP PGRPISVGHG MDSRPAMAIF ELLDYIVNEP PPKLPSGVFS SDFQEFVNKC LIKNPAERAD LKLLMNHAFI KRSEGEEVDF AGWLCRTLRL KQPSTPTRTA V
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
Gene References into Functions
  1. MK2/3 cascade plays a strategic role in controlling synaptic plasticity and cognition. PMID: 25134715
  2. MK2 attenuates dendritic cell-mediated Th1 differentiation and autoimmune encephalomyelitis. PMID: 26078274
  3. analysis of p38-MK2-activated Rsk signaling in toll-like receptor-stimulated dendritic cells PMID: 25332232
  4. MK2 is responsible for resistance of p53-deficient tumors to cisplatin PMID: 24239348
  5. MK2 plays a critical role in the development of apoptosis. PMID: 24304496
  6. A critical role is indicated for p38 effector kinase MK2 in the regulation of tumor necrosis factor (TNF)-alpha messenger RNA (mRNA) translation through the TRIF signaling pathway. PMID: 20375303
  7. Pin1 regulates BPGAP1 function in Rho and Erk signalling, with active Mek2 serving as a novel regulatory scaffold that promotes crosstalk between RhoGAP, Pin1 and Erk in the regulation of cell migration. PMID: 20179103
  8. Results suggest a role for MK2 in the regulation of muscle mass, a role which, at least in part, may be related to determining the subcellular localization of p38 in muscle fibers. PMID: 20049871
  9. MK2, in contrast to its downstream effector molecule, TNF-alpha, has a rather elusive role in T-cell-dependent cutaneous inflammation PMID: 19657354
  10. MK2 signaling has a minor role in skin inflammation [commentary] PMID: 20081887
  11. MK2 stabilizes p38 MAPK through its C terminus and that MK2 catalytic activity does not contribute to this stabilization. PMID: 12052889
  12. MK2 has a role in cytokine expression and in cell migration [review] PMID: 12440954
  13. our findings demonstrate that MEK2 is not necessary for the normal development of the embryo and T-cell lineages, suggesting that the loss of MEK2 can be compensated for by MEK1. PMID: 12832465
  14. MK2 inhibits the mRNA destabilizing activity of tristetraprolin and, in parallel, codegradation of tristetraprolin together, with the target mRNA resulting in increased cellular levels of tristetraprolin. PMID: 16508014
  15. MK2-dependent insolubilization of Hsp25 oligomers has a role in susceptibility to cell stress PMID: 16840785
  16. Fyn-calcineurin-NFATc2 and MEK2-ERK1/2 are two independent signaling pathways that cooperate to control T cell receptor-mediated ICOS induction PMID: 16880206
  17. These results indicate a critical role of p38-MK2-dependent induction of MKP-1 in innate PMID: 16978838
  18. MK2 mediates the incorporation of p38 into the pre-existing complex of hsp27 with Akt PMID: 17015449
  19. MK2-HSP27 pathway is a target for therapeutic control of localized inflammatory diseases. PMID: 17576778
  20. MK2 critically promotes atherogenesis by fostering foam cell formation and recruitment of monocytes/macrophages into the vessel wall. PMID: 17885219
  21. p38 MAP kinase and its downstream effector MK2 mediate lung permeability in ventilator associated lung injury by regulating HSP25 phosphorylation and actin cytoskeletal remodeling. PMID: 19240800
  22. MK2 deficient mouse macrophages produce markedly decreased levels TNF-alpha than wild type macrophages. PMID: 19359247
  23. MK2 has a role in the early stages of tumor promotion through regulation of both the inflammatory response and apoptosis of DNA-damaged cells PMID: 19808857

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Subcellular Location Cytoplasm, Membrane, Peripheral membrane protein
Protein Families Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
Tissue Specificity Expressed in adult intestine, kidney, liver, lung, pancreas, spleen, thymus, and at high levels in the neonatal brain. Lower expression is found in adult brain and heart.
Database Links

KEGG: mmu:26396

STRING: 10090.ENSMUSP00000121111

UniGene: Mm.275436

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