Recombinant Mouse Telomerase reverse transcriptase(Tert),partial

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Code CSB-EP023391MO
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Tert
Uniprot No. O70372
Research Area others
Alternative Names Tert; Telomerase reverse transcriptase; EC 2.7.7.49; Telomerase catalytic subunit
Species Mus musculus (Mouse)
Source E.coli
Expression Region 595-928aa
Target Protein Sequence EVRHHQDTWLAMPICRLRFIPKPNGLRPIVNMSYSMGTRALGRRKQAQHFTQRLKTLFSMLNYERTKHPHLMGSSVLGMNDIYRTWRAFVLRVRALDQTPRMYFVKADVTGAYDAIPQGKLVEVVANMIRHSESTYCIRQYAVVRRDSQGQVHKSFRRQVTTLSDLQPYMGQFLKHLQDSDASALRNSVVIEQSISMNESSSSLFDFFLHFLRHSVVKIGDRCYTQCQGIPQGSSLSTLLCSLCFGDMENKLFAEVQRDGLLLRFVDDFLLVTPHLDQAKTFLSTLVHGVPEYGCMINLQKTVVNFPVEPGTLGGAAPYQLPAHCLFPWCGLLL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 54.1kDa
Protein Length Partial
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis (By similarity).
Gene References into Functions
  1. Par-4 activation and binding to TERT are key steps required for inducing the apoptosis of islet beta cells under high-glucose/fatty acid conditions in type 2 diabetes. PMID: 30186877
  2. Tert-deficient but not Terc-deficient mice develop hepatocyte injury and frank steatosis when challenged with liquid high-fat diet. PMID: 28741793
  3. identification of a subset of hepatocytes that expresses high levels of telomerase and show that this hepatocyte subset repopulates the liver during homeostasis and injury PMID: 29618815
  4. These preclinical data in mouse models and human cells provide a strong rationale for the development of pharmacological approaches to target BMI1-mediated mitochondrial regulation and protection from DNA damage to sustain the regenerative potential of the skeletal muscle in conditions of chronic muscle wasting. PMID: 28757168
  5. Reactivation of Tert in the hippocampus was sufficient to normalize the depressive but not the aggressive behaviors of Tert(-/-) mice. Conversely, re-expression of Tert in the medial prefrontal cortex (mPFC) reversed the aggressive but not the depressive behavior of Tert(-/-) mice. PMID: 27300262
  6. Nrf2-driven TERT regulates pentose phosphate pathway in glioblastoma. PMID: 27148686
  7. TERT has a role in neointima formation through epigenetic regulation of proliferative E2F1 target gene expression in smooth muscle cells. PMID: 27932351
  8. these findings support a model in which gain of TERT function modulates mTORC1 activity and induces autophagy. PMID: 27545609
  9. Regarding extratelomeric activities, our results showed a decrease of 64, 38 and 25% in the transcription of c-Myc, Cyc-D1 and TERT, respectively (p<0.05) after AZT treatment. Furthermore, we found an effect on cell migration, reaching an inhibition of 48% (p<0.05) and a significant passage-dependent increase on cell doubling time during treatment PMID: 27633795
  10. Results suggest that in mature Purkinje neurons, TERT is present both in the nucleus and in mitochondria, where it may participate in adaptive responses of the neurons to excitotoxic and radiation stress PMID: 26374457
  11. Wnt10a/beta-catenin signaling pathway is able to exacerbate keloid cell proliferation and inhibit the apoptosis of keloid cells through its interaction with TERT. PMID: 27771714
  12. This study reports the characterisation of two novel mouse TERT splice variants, Ins-i1[1-102] (Insi1 for short) and Del-e12[1-40] (Dele12 for short) that have not been previously described. Insi1 represents an in-frame insertion of nucleotides 1-102 from intron 1, encoding a 34 amino acid insertion at amino acid 73. PMID: 26787169
  13. TERT may promote gastric cancer metastasis through the TERT-miR-29a-ITGB1 regulatory pathway. PMID: 26903137
  14. TERT switches macrophages towards M1 phenotype by regulating NF-kappaB signaling, but has limited effect on M2 macrophages polarization in vitro. PMID: 26725521
  15. The results suggest that the mouse endometrial epithelium and vasculature are foci of stem/progenitor activity expressing mTert. PMID: 26740067
  16. miR-195 overexpressed in old mesenchymal stem cells (OMSCs) induces stem cell senescence deteriorating their regenerative ability by directly deactivating telomerase reverse transcriptase (Tert), and abrogation of miR-195 can reverse stem cell aging. PMID: 26390028
  17. These findings indicate that telomerase gene therapy represents a novel therapeutic strategy to treat aplastic anemia provoked or associated with short telomeres. PMID: 26903545
  18. findings identified a key role for TERT in fibroblast proliferation and survival essential for pulmonary fibrosis PMID: 26555817
  19. miR-512-5p suppresses tumor growth by targeting TERT in telomerase positive head and neck squamous cell carcinoma in vitro and in vivo. PMID: 26258591
  20. Telomerase deficiency triggers alveolar stem cell replicative senescence-associated low-grade inflammation. PMID: 26518879
  21. high telomerase expression is a fundamental characteristic of germline stem cells. PMID: 26584619
  22. data suggest that S1P binding to hTERT allosterically mimicks phosphorylation, promoting telomerase stability and hence telomere maintenance, cell proliferation, and tumor growth. PMID: 26082434
  23. Telomerase may direct Pol I transcription in oncogenic and regenerative hyperproliferation. PMID: 25118183
  24. Tert expression confers cardioprotection in the adult mouse heart after acute myocardial infarction. PMID: 25519492
  25. TERT is a regulator of MYC stability in cancer. Reactivation of TERT, a direct transcriptional MYC target in tumors, provides a feed-forward mechanism to potentiate MYC-dependent oncogenesis. PMID: 25893605
  26. Pharmacologically relevant doses of atorvastatin resulted in a 6-fold increase of telomerase activity in mouse PBMCs and CD4 T cells. Transgenic GFP-mTert reporter mice had 30% to 15% less telomerase-positive lymphocytes during the first 5 months of age. PMID: 25127175
  27. Studies indicate that reverse transcriptase (RT) enzyme highly expressed in mouse embryos and mouse and human cancer cells and repressed in somatic differentiated healthy cells. PMID: 25586649
  28. The overexpression of Zfp637 markedly increases mTERT expression and telomerase activity, maintains telomere length, and inhibits H2O2 and D-galactose-induced senescence. PMID: 25032857
  29. Telomerase exerts telomere-independent effects on pulmonary artery smooth muscle cell growth in pulmonary hypertension. PMID: 25550449
  30. These findings establish a functional link between endoplasmic reticulum stress and telomerase. PMID: 24119029
  31. TERT, combined with beta-catenin and BRG1, serves as a transcriptional complex, which binds the FAS ligand (FASL) promoter to upregulate FASL expression, leading to an elevated immunomodulatory function. PMID: 24401839
  32. These data indicate that TERT plays an extratelomeric role in the reprogramming process, but its function is dispensable. PMID: 24733392
  33. Once HIV production had reached a peak (7 dpi), the telomerase activity decreased, showing levels similar to those of noninfected cells PMID: 24254728
  34. Overexpression of TERT in mesenchmal stem cells resulted in increased cell proliferation. PMID: 22884695
  35. TERT expression was up-regulated by a histone deacetylase inhibitor, while the induction of TERT in lung fibroblasts was associated with the binding of acetylated histone H3K9 to the TERT promoter region. PMID: 23526226
  36. calorie restriction attenuates telomere erosion associated to aging and that synergizes with TERT over-expression in increasing "health span" and extending mouse longevity PMID: 23349740
  37. prepared two mutant forms of the PhSurv-PhTERT tandem with two or four Sp1 sites removed from the distal "long" PhSurv promoter PMID: 23056318
  38. Study identified the cells responsible for cardiac telomerase activity, demonstrates a significant diminution with age. PMID: 22919071
  39. mir498 has a role in 1,25-Dihydroxyvitamin D3 suppression of telomerase expression and cancer growth PMID: 23055531
  40. Data show that AUF1 binds and strongly activates the transcription promoter for telomerase catalytic subunit Tert. PMID: 22633954
  41. Essential role for telomerase in chronic myeloid leukemia induced by BCR-ABL in mice. PMID: 22408137
  42. telomerase plays a role in the aging of nondividing cells PMID: 22533433
  43. findings show beta-catenin regulates Tert expression through interaction with Klf4, a core component of the pluripotency transcriptional network; beta-Catenin binds to the Tert promoter in a mouse intestinal tumor model PMID: 22723415
  44. Data provide evidence that telomere dysfunction plays a critical role in prostate cancer initiation and progression, permitting acquisition of and selection for cancer-relevant genomic events upon telomerase reactivation. PMID: 22341455
  45. Silencing transgenic TERT expression or inhibiting Wnt signaling through systemic expression of the Wnt inhibitor Dkk1 in either TERT transgenic mice or in a mouse model of HIVAN results in marked normalization of podocytes PMID: 22138751
  46. Selenium and benzene can upregulate the telomerase activity in mouse lymphocytes in vivo. PMID: 19080380
  47. Hippocampal telomerase is involved in the modulation of depression-related behaviors, possibly by regulating adult neurogenesis. PMID: 21865469
  48. Primitive hematopoietic Tert(-/-) cells lacking telomerase activity exhibit signs of enhanced DNA damage. PMID: 21730353
  49. Results indicate that both telomerase reverse transcriptase and telomerase RNA are haploinsufficient and that their deficiency leads to telomere shortening, which limits tissue renewal. PMID: 21464209
  50. The results of this study provide the first experimental evidence that telomere shortening, despite impairing adult neurogenesis and maintenance of post-mitotic neurons, can slow down the progression of amyloid plaque pathology in Alzheimer's disease. PMID: 21672962
  51. Pioglitazone treatment up-regulates telomerase activity, telomere-stabilizing proteins and reduces senescence markers in vascular cells. PMID: 21396644
  52. Atm and Tert doubly deficient mice demonstrated increased progression of aging and had shorter lifespans than Atm-null mice, while Tert alone was insufficient to affect lifespan. PMID: 21297001
  53. Results suggest that oxidant stress might affect myocardial telomerase activity and telomere-associated proteins. PMID: 21195081
  54. PITX1 suppresses TERT transcription through direct binding to the TERT promoter, which ultimately regulates telomerase activity PMID: 21300782
  55. Tumor volumes significantly decreased, and appearances of tumor formation in mice were delayed in the TERT-knockdown embryonic stem cells. PMID: 20486780
  56. TERT deficiency in bone marrow-derived macrophages attenuates Ang II-induced abdominal aortic aneurysms formation in mice. These results point to a previously unrecognized role of TERT in the pathogenesis of abdominal aortic aneurysms. PMID: 21088250
  57. TERT expression prevents macrophage senescence and may have important implications for the development of atherosclerosis. PMID: 21106948
  58. mTert(+) cells give rise to all differentiated intestinal cell types, persist long term, and contribute to the regenerative response following injury. PMID: 21173232
  59. Telomerase reactivation extends telomeres, reduces DNA damage signalling and associated cellular checkpoint responses, allows resumption of proliferation in quiescent cultures, and eliminates degenerative phenotypes across multiple organs PMID: 21113150
  60. Hypertonic stress activates mTERT transcription via the activation and recruitment of NFAT5 to the mTERT promoter. PMID: 20937271
  61. TERT was up-regulated in a mouse model of oxygen-induced retinopathy. PMID: 19575911
  62. Vascular injury induces de novo expression of TERT in intimal smooth muscle cells via activation of nuclear factor kappa B and upregulation of c-Myc,leading to hyperplasia. PMID: 20864668
  63. Direct shRNA-mediated knockdown of Hif1alpha expression confirmed that suppression of hypoxia inducible factor 1 alpha levels was accompanied by a reduction in both Tert mRNA and telomerase activity levels. PMID: 20643931
  64. the hTERT promoter was strongly activated in discrete steps, revealing a critical difference in human and mouse cell reprogramming PMID: 20354136
  65. DeltaNp63alpha regulates mTERT activity at the transcriptional level via the mTERT promotor site, and also through the induction of spliced mRNA mTERT isoforms. PMID: 20157588
  66. results suggest that TERT is constitutively expressed in the hippocampus and the olfactory bulbs, and that it is important for regulating normal brain functions. PMID: 19685288
  67. deregulated GSK3beta sustains gastrointestinal cancer cells survival through modulation of hTERT and telomerase PMID: 19903789
  68. Telomerase reverse transcriptase-dependent telomere equilibration mitigates tissue dysfunction in mTert heterozygotes PMID: 19841238
  69. mTERT does not act as a transcription factor or play a role in the DNA damage response. PMID: 19850716
  70. presence of the P6.1 stem-loop in telomerase RNA and its importance for the assembly and enzymatic activity of the telomerase complex PMID: 11788723
  71. Preferential maintenance of critically short telomeres in mammalian cells heterozygous for mTert PMID: 11904422
  72. Under steady-state conditions, the levels and functionality of hematopoietic-committed or multipotent progenitors were not affected by telomerase deficiency. PMID: 11929765
  73. role of Ets-mediate transactivation in activation of telomerase by EGF PMID: 12037663
  74. Cooperation between p53 mutation and high telomerase transgenic expression in spontaneous cancer development. PMID: 12242304
  75. one role for telomerase in T cells may be to renew or extend replicative potential via the rejuvenation of telomere length. PMID: 12461078
  76. One role for telomerase in the HSC is to partially counter the rate of telomere shortening during division of HSCs, thereby preventing premature loss of telomere function and providing added replicative capacity. PMID: 12663456
  77. exogenous wtBRCA1 strongly suppressed TERT promoter activity in various cell lines PMID: 14612409
  78. TERT is inducible in postmitotic neurons after ischemic brain injury and prevents NMDA neurotoxicity through shift of the cytosolic free Ca2+ into the mitochondria, and thus plays a protective role in ameliorating ischemic neuronal cell death PMID: 14960598
  79. limiting TERT levels play a key role in the maintenance of genome integrity, with important ramifications for the maintenance of short telomeres in human cancer and aging PMID: 15079066
  80. Lck-Tert mouse lines showed higher incidences of spontaneous T-cell lymphoma than the corresponding age-matched wild-type controls, indicating that constitutive expression of Tert promotes lymphoma. PMID: 15121848
  81. telomerase activity is regulated by glutathione PMID: 15184392
  82. In knockout mice, negative impact of telomere shortening on organ homeostasis and organismal survival can surpass the beneficial effects of telomere shortening on suppression of tumor growth in the setting of chronic organ damage. PMID: 15608677
  83. Ectopic mTERT expression protects embryonic stem cells against cell death during differentiation. PMID: 15687103
  84. Telomere dysfunction induces a robust compensatory response to rescue impaired germ cell function through the induction of survival signals. PMID: 15860505
  85. the telomerase activity of cells in various stages of differentiation was unaffected by aging and, notably, remained high in the alpha6-integrin-positive side Population PMID: 15919739
  86. findings show that telomere length, as well as the catalytic component of telomerase, Tert, are critical determinants in the mobilization of epidermal stem cells PMID: 16037417
  87. high rate of genomic rearrangements in telomerase deficient ES cells may reflect the cultured cells' gained ability to protect chromosome ends with eroded telomeres allowing them to escape "end crisis" PMID: 16131840
  88. there was no effect on mTERT expression or mTERT promoter activity by AP-1 overexpression in mouse fibroblasts. (TERT) PMID: 16135795
  89. GC-box-mediated, human-specific mechanism for TERT repression is impaired in human cancers PMID: 16344462
  90. The 1 kb promoter upstream of the initiating ATG codon of mTert contains all the regulatory elements to control telomerase expression in ES cells during in vitro loss of pluripotency. PMID: 16457732
  91. Abrogates the growth-inhibitory effect of transforming growth factor-beta in embryonic fibroblasts. PMID: 16501597
  92. Mice lacking telomerase activity show hypertension as a result of an increase in plasma ET-1 levels, which is a consequence of ECE-1 overexpression. A direct link between telomerase activity and hypertension is reported. PMID: 16831983
  93. telomerase activity controls the glycolytic pathway, potentially altering the energy state of tumor cells and thereby modulating tyrosinase activity and melanin production PMID: 16847266
  94. These results highlight telomerase as a mediator of Myc-induced papillomatosis and suggest telomerase as a putative therapeutic target for Myc-dependent lesions. PMID: 16880523
  95. These results show for the first time that IP6 represses telomerase activity in prostate cancer cells by posttranslational modification of TERT via the deactivation of Akt and PKCalpha. PMID: 16979586
  96. Murine telomere homeostasis or genetic stability does not depend on mPif1. PMID: 17130244
  97. DNA-dependent protein kinase deficiency does not reduce apoptosis, tissue atrophy, or p53 activation in late-generation mTerc(-/-) tissues but rather moderately exacerbates germ cell apoptosis and testicular degeneration PMID: 17145779
  98. telomerase limits the accumulation of telomere dysfunction, the evolution of excessive aneuploidy, and the activation of p53-independent checkpoints suppressing hepatocarcinogenesis. PMID: 17433324
  99. Large defects of type I allergic response in Tert knockout mice. PMID: 17456801
  100. TERT induction is associated with increased survival of lung fibroblasts, which favors the development of fibrosis instead of injury resolution. PMID: 18008008

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Subcellular Location Nucleus, nucleolus, Nucleus, nucleoplasm, Nucleus, Chromosome, telomere, Cytoplasm, Nucleus, PML body
Protein Families Reverse transcriptase family, Telomerase subfamily
Tissue Specificity High activity in intestine, liver and testis, moderate in lung, very low in muscle, heart and brain.
Database Links

KEGG: mmu:21752

STRING: 10090.ENSMUSP00000022104

UniGene: Mm.10109

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