Recombinant Mouse Tyrosine-protein kinase JAK1 (Jak1) is expressed in E. coli with an N-terminal 6xHis-tag that simplifies purification. This partial protein covers amino acids 848-1152 and shows purity levels above 90%, confirmed through SDS-PAGE analysis. Designed strictly for research purposes, this product offers scientists a useful tool for investigating kinase function and signaling pathways.
JAK1 appears to play a vital role in how various cytokines and growth factors communicate within cells. Being part of the Janus kinase family, JAK1 seems responsible for phosphorylating and activating STAT proteins—signal transducer and activator of transcription proteins that carry messages from cell surfaces to the nucleus. Understanding this protein may be essential for grasping how cells respond and regulate themselves, particularly in immune and blood-forming systems.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. In Vitro Kinase Activity Assays
This recombinant JAK1 kinase domain (848-1152aa) works well for setting up and fine-tuning kinase activity assays with different substrates and ATP analogs. The purified protein makes controlled biochemical studies possible, helping researchers characterize kinase parameters like Km values, substrate specificity, and reaction kinetics. Its N-terminal 6xHis tag makes purification straightforward and allows for immobilization in high-throughput screening setups. These assays might serve as fundamental tools for understanding JAK1's enzymatic behavior in controlled laboratory conditions.
2. JAK1-Specific Antibody Development and Validation
The highly pure recombinant JAK1 kinase domain could work as an immunogen for creating monoclonal or polyclonal antibodies that target this particular JAK1 region. The well-defined 848-1152aa sequence gives researchers a characterized antigen for antibody production and follow-up validation work. The 6xHis tag makes antigen purification and quantification relatively simple for immunization procedures. Researchers can then validate any resulting antibodies using this same recombinant protein in ELISA, Western blot, and similar immunoassays.
3. Protein-Protein Interaction Studies
This JAK1 kinase domain appears useful in pull-down assays and binding experiments aimed at identifying and characterizing proteins that interact with this specific region. The N-terminal 6xHis tag makes it possible to attach the protein to nickel-based resins for affinity purification experiments using cell lysates or purified proteins. The 305-amino acid fragment lets researchers focus on interactions that the kinase domain specifically mediates. Such studies might help scientists map where binding occurs and measure how strong these protein-protein interactions really are.
4. Structural and Biophysical Characterization
The purified recombinant JAK1 kinase domain supplies material for structural biology techniques including X-ray crystallography, NMR spectroscopy, and cryo-electron microscopy studies. Its high purity and clearly defined sequence boundaries make it suitable for biophysical analyses like dynamic light scattering, differential scanning calorimetry, and circular dichroism spectroscopy. The E. coli expression system and 6xHis tag help produce the large quantities needed for structural work. These approaches could reveal important details about the three-dimensional structure and how the JAK1 kinase domain moves and changes shape.
