Code | CSB-RA267127A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IF | 1:20-1:200 |
In order to develop a JAK2 recombinant monoclonal antibody for detecting human JAK2 protein, CUSABIO takes four key steps. The production process involves sequencing the JAK2 monoclonal antibody gene, cloning the gene into a plasmid vector, introducing the recombinant vector into a host cell line, and purifying the JAK2 recombinant monoclonal antibody from the cell culture supernatant using affinity chromatography. The JAK2 monoclonal antibody is derived from the JAK2 antibody-producing hybridomas, and its synthesis includes the immunization of an animal with a synthesized peptide derived from human JAK2. The JAK2 recombinant monoclonal antibody is recommended for use in ELISA and IF applications.
The JAK2 protein is a kinase enzyme that plays a crucial role in the intracellular signaling pathways of various cytokines and growth factors. Upon activation by ligand binding to cell surface receptors, JAK2 phosphorylates specific tyrosine residues on the receptor, leading to the recruitment and phosphorylation of downstream signaling molecules, including STAT proteins. The activated STATs then dimerize, translocate to the nucleus, and activate the transcription of target genes involved in cell proliferation, differentiation, and survival. JAK2 also plays a critical role in erythropoietin (EPO) signaling, which is essential for the regulation of red blood cell production. Mutations in the JAK2 gene have been implicated in various diseases, including myeloproliferative neoplasms and autoimmune disorders.
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