Code | CSB-RA546523A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The creation of the RHOA recombinant monoclonal antibody involves a meticulous and thorough process to ensure its exceptional quality and specificity. Initially, B cells are isolated from the spleen of an immunized animal using the synthesized peptide derived from human RHOA as the immunogen. The RNA is then extracted from the B cells and converted into cDNA through reverse transcription. The RHOA antibody genes are amplified using specific primers targeting the antibody constant regions and inserted into an expression vector. This vector is subsequently introduced into host cells through transfection, allowing for the production of the RHOA recombinant monoclonal antibody. After a period of cell culture, the antibody is harvested from the cell culture supernatant and subjected to meticulous purification using affinity chromatography, resulting in a highly purified form suitable for various applications. Rigorous characterization assays, including ELISA and IHC analysis, are performed to validate the antibody's specificity and functionality in detecting human RHOA protein. The stringent production process ensures the development of a reliable and effective RHOA recombinant monoclonal antibody, which serves as a valuable tool in diverse research related to RHOA.
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