RHOA Antibody

Datasheet
Code CSB-PA042169
Product Type Polyclonal Antibody
Size US$297
Uniprot No. P61586
Image
  • Western blot analysis of extracts from HT29 and HepG2 cells using RhoA Antibody.
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunogen Peptide sequence around aa.180-184(Q-A-R-R-G) derived from Human RhoA.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous level of total RhoA.
Tested Applications ELISA,WB;WB:1:500-1:1000
Relevance

 Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality Polyclonal
Alias ARHA, ARH12, RHO12, RHOH12
Immunogen Species Homo sapiens (Human)
Target Names RHOA
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis
Subcellular Location Cell membrane, Lipid-anchor, Cytoplasmic side, Cytoplasm, cytoskeleton, Cleavage furrow, Cytoplasm, cell cortex, Midbody, Cell projection, lamellipodium
Protein Families Small GTPase superfamily, Rho family
Database Links

HGNC: 667

OMIM: 165390

KEGG: hsa:387

STRING: 9606.ENSP00000400175

UniGene: Hs.247077

Pathway cAMP signaling pathway
Chemokine signaling pathway
mTOR signaling pathway
TGF-beta signaling pathway
Wnt signaling pathway
Focal adhesion
Regulation of actin cytoskeleton
Vascular smooth muscle contraction
Adherens junction
Ras signaling pathway
Leukocyte transendothelial migration
NOD-like receptor signaling pathway
T cell receptor signaling pathway
cGMP-PKG signaling pathway
Oxidative Phosphorylation
Phospholipase D signaling pathway
Rap1 signaling pathway
Sphingolipid signaling pathway
Endocytosis
Inhibitors of axonal regeneration
Neurotrophin signaling pathway

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