CD247

CD247 is a protein found on the surface of T cells and natural killer cells. It consists of a large extracellular cytoplasmic domain and a small short cytoplasmic tail. It interacts with other molecules in the T cell receptor (TCR) complex, forming a complete signaling complex and playing a pivotal role in signal transduction. When the T cell receptor binds to an antigen, CD247 interacts with other CD3 chains, such as CD3γ, CD3δ, CD3ε, as well as T cell receptor α and β chains, forming the TCR-CD3 complex. This complex activates T cells through intracellular signaling pathways, leading to an immune response against the antigen. Phosphorylation of CD247 and its interaction with other intracellular signaling molecules are critical steps in T cell receptor signal transduction. It helps regulate and activate T cells, enabling them to mount effective immune responses against infections, tumor cells, and other abnormal cells. Therefore, CD247 plays a crucial role in maintaining the function of the immune system, resisting diseases, and preserving health.

Validated Data

SDS

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

ELISA

Activity: Measured by its binding ability in a functional ELISA. Immobilized PD-L1 at 2 μg/ml can bind Anti- PD-L1 mouse monoclonal antibody(CSB-MA878942A1m,antigen from E.coli), the EC₅₀ of human PD-L1 protein is 1.252-1.653 ng/mL

HPLC

The purity of CD274 was greater than 90% as determined by SEC-HPLC.

● CD274 Recombinant Monoclonal Antibody CSB-RA977797A0HU

Validated Data

Western Blot
Positive WB detected in: HepG2 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate
All lanes: CD274 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 21, 21 kDa
Observed band size: 45 kDa

IHC

IHC image of CSB-RA977797A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

The following CD247 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

CD247 Antibodies

CD247 Proteins

CD247 ELISA Kits

CD247 Antibodies

CD247 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA004904GA01HU	CD247 Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA070253	Phospho-CD247 (Y142) Antibody	Human,Mouse,Rat,Monkey	WB, IHC, ELISA
CSB-PA070254	CD247 Antibody	Human,Mouse,Rat,Monkey	WB, IHC, ELISA
CSB-PA273375	CD247 Antibody	Human	IHC,ELISA
CSB-PA10499A0Rb	CD247 Antibody	Human	ELISA, WB, IHC, IF, IP
CSB-PA10499B0Rb	CD247 Antibody, HRP conjugated	Human	ELISA
CSB-PA10499C0Rb	CD247 Antibody, FITC conjugated	Human
CSB-PA10499D0Rb	CD247 Antibody, Biotin conjugated	Human	ELISA
CSB-RA244537A0HU	CD247 Recombinant Monoclonal Antibody	Human	ELISA, FC

CD247 Proteins

CD247 Proteins for Oryctolagus cuniculus (Rabbit)

Code	Product Name	Source
CSB-YP866148RB CSB-EP866148RB CSB-BP866148RB CSB-MP866148RB CSB-EP866148RB-B	Recombinant Rabbit T-cell surface glycoprotein CD3 zeta chain (CD247), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD247 Proteins for Sus scrofa (Pig)

Code	Product Name	Source
CSB-YP895010PI CSB-EP895010PI CSB-BP895010PI CSB-MP895010PI CSB-EP895010PI-B	Recombinant Pig T-cell surface glycoprotein CD3 zeta chain (CD247), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD247 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-CF004904HU	Recombinant Human T-cell surface glycoprotein CD3 zeta chain (CD247)	in vitro E.coli expression system
CSB-YP004904HU1 CSB-EP004904HU1 CSB-BP004904HU1 CSB-MP004904HU1 CSB-EP004904HU1-B	Recombinant Human T-cell surface glycoprotein CD3 zeta chain (CD247), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD247 Proteins for Ovis aries (Sheep)

Code	Product Name	Source
CSB-CF004904SH	Recombinant Sheep T-cell surface glycoprotein CD3 zeta chain (CD247)	in vitro E.coli expression system
CSB-YP004904SH1 CSB-EP004904SH1 CSB-BP004904SH1 CSB-MP004904SH1 CSB-EP004904SH1-B	Recombinant Sheep T-cell surface glycoprotein CD3 zeta chain (CD247), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CD247 ELISA Kit

CD247 ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-EL004904HU	Human T-cell surface glycoprotein CD3 zeta chain (CD247) ELISA kit	serum, plasma, tissue homogenates, cell lysates	0.039 ng/mL

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CD247

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