Receptor tyrosine-protein kinase erbB-2 (also known as ERBB2) is a protein encoded by ERBB2 gene. The protein encoded by this gene is also known as epidermal growth factor receptor 2 (HER2) and is a member of the epidermal growth factor receptor family (EGFR). ERBB2 protein is a transmembrane glycoprotein with tyrosine kinase activity, which has a molecular weight of approximately 185 kDa, also known as p185. HER2 plays a crucial role in tissue development and is essential for normal embryonic development. Notably, HER2 is usually under- or non-expressed in normal adult body tissues. Over-expressed HER2 often causes biological dysfunction, and many studies have shown that HER2 expression is closely related to the occurrence of many tumors.
Currently, no ligand has been identified for ERBB2. HER2 can heterodimerise with any of the other three receptors (such as HER3 or HER4) and is also considered to be the preferred dimerisation partner of the other ERBB receptors. HER2 dimerization results in a conformational change that activates intracellular tyrosine kinase activity, thereby initiating a variety of signaling pathways mainly including RAS-RAF-MEK-ERK for cell proliferation and PI3K-AKT-mTOR pathway for cell survival.
● Recombinant Human Receptor tyrosine-protein kinase erbB-2(ERBB2),partial (Active) (CSB-MP007763HU)
Validated Data
Greater than 95% as determined by SDS-PAGE.
Immobilized HER2 at 2 μg/ml can bind Trastuzumab, the EC50 is 2.179-2.825 ng/ml.
● ERBB2 Recombinant Monoclonal Antibody CSB-RA007763MA1HU
Validated Data
The Binding Activity of Human ERBB2 with Anti-ERBB2 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ERBB2 (CSB-MP007763HU) at 2 μg/mL can bind Anti-ERBB2 recombinant antibody, the EC50 is 2.455-3.565 ng/mL.
● ERBB2 Recombinant Monoclonal Antibody CSB-RA007763MA2HU
Validated Data
IHC image of CSB-RA007763MA2HU diluted at 1:600 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
The following ERBB2 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
ERBB2 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA007763GA01HU | ERBB2 Antibody |
Human | ELISA,WB,IHC |
CSB-PA09759C0Rb | ERBB2 Antibody, FITC conjugated |
Human | |
CSB-PA09759D0Rb | ERBB2 Antibody, Biotin conjugated |
Human | ELISA |
CSB-PA007763KA01HU | ERBB2 Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA09759B0Rb | ERBB2 Antibody, HRP conjugated |
Human | ELISA |
CSB-PA09759A0Rb | ERBB2 Antibody |
Human | ELISA, IHC |
CSB-PA846722 | Phospho-ERBB2 (Tyr877) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC,IF |
CSB-PA281422 | Phospho-ERBB2 (Tyr1221/Tyr1222) Antibody |
Human | ELISA,WB,IF |
CSB-PA997735 | Phospho-ERBB2 (Tyr1248) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC,IF |
CSB-PA200841 | ERBB2 (Ab-877) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA899547 | ERBB2 (Ab-1221/1222) Antibody |
Human | ELISA,WB,IHC,IF |
CSB-PA115264 | ERBB2 (Ab-1248) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC,IF |
CSB-PA761682 | Phospho-ERBB2 (Tyr1112) Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-MA000200 | ERBB2 Monoclonal Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-MA000237 | ERBB2 Monoclonal Antibody |
Human | ELISA,WB |
CSB-PA000577 | Phospho-ERBB2 (Y1221/Y1222) Antibody |
Human,Mouse,Rat | WB, IHC, IP, ELISA |
CSB-PA000578 | Phospho-ERBB2 (Y877) Antibody |
Human,Mouse,Rat | WB, IHC, IP, ELISA |
CSB-PA000733 | Phospho-ERBB2 (Y1221/Y1222) Antibody |
Human,Mouse,Rat,Monkey | WB, IHC, IF, ELISA |
CSB-PA003405 | ERBB2 Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA003407 | ERBB2 Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA008077 | Phospho-ERBB2 (Y1248) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA008078 | Phospho-ERBB2 (Y1112) Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA008079 | Phospho-ERBB2 (T686) Antibody |
Human,Mouse,Rat | IF, ELISA |
CSB-PA008081 | ERBB2 Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA008088 | ERBB2 Antibody |
Human,Mouse,Rat | IF, ELISA |
CSB-PA008089 | ERBB2 Antibody |
Human,Mouse,Rat | IF, ELISA |
CSB-PA008092 | ERBB2 Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA972166 | ERBB2 Antibody |
Human | ELISA,WB |
CSB-RA260392A0HU | ERBB2 Recombinant Monoclonal Antibody |
Human | ELISA, IHC, IF |
ERBB2 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP007763MO CSB-EP007763MO CSB-BP007763MO CSB-MP007763MO CSB-EP007763MO-B |
Recombinant Mouse Receptor tyrosine-protein kinase erbB-2(Erbb2),partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ERBB2 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP007763RA CSB-EP007763RA CSB-BP007763RA CSB-MP007763RA CSB-EP007763RA-B |
Recombinant Rat Receptor tyrosine-protein kinase erbB-2(Erbb2),partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ERBB2 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP007763HU CSB-BP007763HU CSB-MP007763HU CSB-EP007763HU-B |
Recombinant Human Receptor tyrosine-protein kinase erbB-2(ERBB2),partial |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP007763HU | Recombinant Human Receptor tyrosine-protein kinase erbB-2(ERBB2),partial |
E.coli |
CSB-EP007763HU1 | Recombinant Human Receptor tyrosine-protein kinase erbB-2(ERBB2),partial |
E.coli |
CSB-EP007763HU3 | Recombinant Human Receptor tyrosine-protein kinase erbB-2(ERBB2),partial |
E.coli |
CSB-MP007763HU | Recombinant Human Receptor tyrosine-protein kinase erbB-2(ERBB2),partial (Active) |
Mammalian cell |
ERBB2 Proteins for Canis lupus familiaris (Dog) (Canis familiaris)
Code | Product Name | Source |
---|---|---|
CSB-YP007763DO CSB-EP007763DO CSB-BP007763DO CSB-MP007763DO CSB-EP007763DO-B |
Recombinant Dog Receptor tyrosine-protein kinase erbB-2(ERBB2) ,partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ERBB2 Proteins for Mesocricetus auratus (Golden hamster)
Code | Product Name | Source |
---|---|---|
CSB-YP736718MRG CSB-EP736718MRG CSB-BP736718MRG CSB-MP736718MRG CSB-EP736718MRG-B |
Recombinant Mesocricetus auratus Receptor tyrosine-protein kinase erbB-2(ERBB2) ,partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ERBB2 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E11161h | Human epidermal growth factor receptor 2 (sp185/HER2) ELISA Kit |
serum, plasma, tissue homogenates, cell lysates | 0.04 ng/mL |