Name: Recombinant Human Receptor tyrosine-protein kinase erbB-2 (ERBB2), partial (Active)
Brand: CUSABIO
SKU: CSB-MP007763HU

Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized HER2 at 2 μg/ml can bind Trastuzumab, the EC₅₀ is 2.179-2.825 ng/ml.

Target Names

ERBB2

Uniprot No.

P04626

Alternative Names

(MLN 19)(Proto-oncogene Neu)(Proto-oncogene c-ErbB-2)(Tyrosine kinase-type cell surface receptor HER2)(p185erbB2)(CD340)(HER2)(MLN19)(NEU)(NGL)

Molecular Characterization

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

23-652aa

Target Protein Sequence

TQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLT

Mol. Weight

70.2 kDa

Protein Length

Partial

Tag Info

C-terminal 6xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant human ERBB2 protein is generated in mammalian cells through the expression of the plasmid containing the target gene that encodes the 23-652aa of the human ERBB2. This protein is fused with 6xHis-tag at the C-terminus. The protein achieves a purity of over 95%, verified by SDS-PAGE, and contains endotoxin less than 1.0 EU/μg, measured via the LAL method. It has been validated to be biologically active. ELISA confirms its specific binding to the Trastuzumab, with an EC₅₀ ranging from 2.179 to 2.825 ng/mL.

Human ERBB2, also known as HER2/neu, a member of the EGFR family, is a transmembrane tyrosine kinase involved in cell signaling pathways that regulate cell proliferation, survival, and differentiation. ERBB2 is particularly notable for its role in various cancers, most prominently breast cancer, where it is overexpressed in approximately 15-30% of cases, leading to aggressive tumor behavior and poor prognosis [1][2][3].

The ERBB2 protein lacks a known ligand but is essential for forming heterodimers with other EGFR family members, particularly with HER3. This dimerization enhances signaling through pathways such as the PI3K/Akt and MAPK pathways, which are critical for promoting cell survival and proliferation [1][4][5][6]. The aberrant activation of these signaling pathways due to ERBB2 overexpression is a significant contributor to oncogenesis and tumor progression [7][8][9].

In addition to its role in cancer, ERBB2 has been implicated in various physiological processes, including cardiac function. Studies have shown that ERBB2 is involved in cardiomyocyte survival and hypertrophy [10][11]. Therapeutically, ERBB2 has become a target for monoclonal antibody treatments, most notably trastuzumab (Herceptin), which has been shown to improve outcomes in patients with ERBB2-positive breast cancer by inhibiting receptor signaling and promoting antibody-dependent cellular cytotoxicity [1][4][12].

References:
[1] M. Karass, R. Bareja, E. Shelkey, P. Vlachostergios, B. Robinson, F. Khani, et al. Oncogenic addiction to erbb2 signaling predicts response to trastuzumab in urothelial cancer, Journal of the National Comprehensive Cancer Network, vol. 17, no. 3, p. 194-200, 2019. https://doi.org/10.6004/jnccn.2018.7264
[2] E. Zhang, M. Cristofanilli, F. Robertson, J. Reuben, Z. Mu, R. Beavis, et al. Genome wide proteomics of erbb2 and egfr and other oncogenic pathways in inflammatory breast cancer, Journal of Proteome Research, vol. 12, no. 6, p. 2805-2817, 2013. https://doi.org/10.1021/pr4001527
[3] Y. Li, Y. Pan, Y. Wei, X. Cheng, B. Zhou, M. Tan, et al. Upregulation of cxcr4 is essential for her2-mediated tumor metastasis, Cancer Cell, vol. 6, no. 5, p. 459-469, 2004. https://doi.org/10.1016/j.ccr.2004.09.027
[4] J. Liu, T. Wang, C. Willson, K. Janardhan, S. Wu, J. Li, et al. Erbb2 regulates med24 during cancer progression in mice with pten and smad4 deletion in the pulmonary epithelium, Cells, vol. 8, no. 6, p. 615, 2019. https://doi.org/10.3390/cells8060615
[5] L. Arias-Romero, O. Villamar-Cruz, A. Pacheco, R. Kosoff, M. Huang, S. Muthuswamy, et al. A rac–pak signaling pathway is essential for erbb2-mediated transformation of human breast epithelial cancer cells, Oncogene, vol. 29, no. 43, p. 5839-5849, 2010. https://doi.org/10.1038/onc.2010.318
[6] X. Liu, Y. Zhang, W. Ren, & G. Rao. Erbb2 gene silencing and its effect on pten in sacc-83 salivary adenoid cystic carcinoma cells, Oncology Reports, vol. 24, no. 5, 2010. https://doi.org/10.3892/or_00000985
[7] E. Wolfson, M. Goldenberg, S. Solomon, A. Frishberg, & R. Pinkas‐Kramarski. Nucleolin-binding by erbb2 enhances tumorigenicity of erbb2-positive breast cancer, Oncotarget, vol. 7, no. 40, p. 65320-65334, 2016. https://doi.org/10.18632/oncotarget.11323
[8] Y. Qi, T. Su, Z. Xia, Y. Jiang, W. Yuan, W. Wang, et al. Gene expression profiles of phaeochromocytomas with erbb2 overexpression reveal a new molecular mechanism tumourigenicity, Clinical Endocrinology, vol. 77, no. 3, p. 399-406, 2012. https://doi.org/10.1111/j.1365-2265.2012.04388.x
[9] S. Karakashev and M. Reginato. Hypoxia/hif1α induces lapatinib resistance in erbb2-positive breast cancer cells via regulation of dusp2, Oncotarget, vol. 6, no. 4, p. 1967-1980, 2015. https://doi.org/10.18632/oncotarget.2806
[10] P. Sysa‐Shah, Y. Xu, X. Guo, F. Belmonte, B. Kang, D. Bedja, et al. Cardiac-specific over-expression of epidermal growth factor receptor 2 (erbb2) induces pro-survival pathways and hypertrophic cardiomyopathy in mice, Plos One, vol. 7, no. 8, p. e42805, 2012. https://doi.org/10.1371/journal.pone.0042805
[11] A. Negro, B. Brar, Y. Gu, K. Peterson, W. Vale, & K. Lee. Erbb2 is required for g protein-coupled receptor signaling in the heart, Proceedings of the National Academy of Sciences, vol. 103, no. 43, p. 15889-15893, 2006. https://doi.org/10.1073/pnas.0607499103
[12] F. Troise, M. Monti, A. Merlino, F. Cozzolino, C. Fedele, I. Krauss, et al. A novel erbb2 epitope targeted by human antitumor immunoagents, Febs Journal, vol. 278, no. 7, p. 1156-1166, 2011. https://doi.org/10.1111/j.1742-4658.2011.08041.x

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

4.0 - 2 reviews

Submit a Review here

Applications : Antigen, Binding assay/Protein-protein interaction

Review: ERBB2 is involved in the regulation of many important functions, including cell growth, differentiation and apoptosis. It is an important participant in human malignant tumors and one of the hot targets for current research on tumors. I used CSB-MP007763HU product for an ELISA experiment, EC50 was 1.212-3.812 ng/ml, and the protein had complete biological activity. Subsequent screening of antibodies resulted in more high-quality antibodies, indicating good protein performance! The subsequent experiment was very smooth!

By Anonymous

Applications : Antigen, Binding assay/Protein-protein interaction

Review: After receiving the product, we conducted SDS detection and functional ELISA verification, and the results showed that the protein purity was higher than the standard, and there were no impurity bands. The binding activity with antibodies was good, and the EC50 was 1.212-3.812 ng/ml. After immunizing the mouse, the serum titer was high, and the ELISA testing effect of the coated protein was good. In-stock products with fast delivery, good packaging, stable protein performance, and good repeatability! We will continue to purchase in large quantities!

By Anonymous

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Target Background

Function

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.; In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

Gene References into Functions

This abnormally sensitive electrochemical sensing performance resulting from anionic porphyrin for DNA sequences specific to HER2 gene will offer considerable promise for tumor diagnosis and treatment PMID: 30340409
Authors showed that mRNA and protein levels of COX2 and HER2 were upregulated in CRC compared with the adjacent tissues. COX2 protein levels and nuclear COX2 expression were correlated with a poor prognosis of CRC patients. COX2 expression was positively associated with HER2 expression. PMID: 29873317
In patients with HER2-positive advanced breast cancer who have been heavily pretreated with anti-HER2 agents and cytotoxic chemotherapy, trastuzumab emtansine (T-DM1) is well tolerated and provided a meaningful progression-free survival of 6 months and an overall survival that has not been reached. PMID: 29326401
The expression of C-Met and HER2 protein in lung adenocarcinoma is highly correlated, and whether it is synergistic in the targeted therapy of lung adenocarcinoma deserves further study. PMID: 29400000
Although ST6GalI overexpression increased HER2 sialylation, corresponding to decreased HER2 phosphorylation, high alpha2,6sialylation enhanced Akt and ERK phosphorylation levels compared to those in the vector cell line; ST6GalI knockdown had the opposite effects. Collectively, these results implicated a functional role of ST6GalI in promoting tumor cell progression and trastuzumab resistance. PMID: 30226606
Study demonstrate that the miR-495 exerts promotive effects on GC chemosensitivity via inactivation of the mTOR signaling pathway by suppressing ERBB2. The study provides reliable evidence supporting the use of miR-495 as a novel potential target in the chemotherapy of GC. PMID: 30147110
In early breast cancer, PIK3CA mutations seem to identify HER2+ patients who are less likely to reach pCR. The clinical implications of PIK3CA mutations tend to vary between exon 9 and exon 20. This mechanism should be explored in further studies. PMID: 29575819
HER2 and HER3 expression was detected in 22.2% and 86.1% of samples, respectively. The frequency of EGFR mutation was 45.7% and was not significantly different between stage 0 and IA1 (40.0% and 48.0%, respectively), suggesting that EGFR mutation does not correlate with cancer progression from stage 0 to IA1. PMID: 29473311
It has been demonstrated that the heterogeneity of HER2 expression accelerated the development of metastases which caused the poor survival of mice with heterogeneous HER2 expression (HER2-60). PMID: 30042341
Her-2/neu amplification increases with increasing grade of breast cancer. A high proportion of Her-2/neu gene amplified cases indicates aggressive disease in that area and need for FISH testing on large scale, which is the gold standard for equivocal cases on immunohistochemistry. PMID: 30060783
Data indicate that the major mechanism is the ability of p140Cap to interfere with ERBB2-dependent activation of Rac GTPase-controlled circuitries. PMID: 28300085
Study showed the expression levels of Gli1 and HER2 were significantly higher in gastric cancer, and they are positively related. HER2 may regulate Gli1 by Akt-mTOR-p70S6K pathway. PMID: 29321573
The combination of immunohistochemical expression of BRCA1, ER, PR, and HER-2/neu and clinicopathological details may be helpful in predicting the individuals more likely to carry BRCA1 mutations and thus selecting the candidate and family members for genetic screening for BRCA1 mutations. PMID: 29567881
In the current settings, HER2/neu is not found to be a prognostic marker in head-and-neck cancers. PMID: 30004046
These results may be commented as HE4 expression rises in patients with HER2/neu amplification. PMID: 30004048
HER2 gene amplification in circulating tumor DNA predicts HER2-positive breast neoplasms resistance to trastuzumab emtansine. PMID: 29700710
Statistical analysis performed in this study did not reveal the significant relationship between HER2 overexpression on the tumor cells and microvessel density in the tumor stroma. PMID: 30334990
Data showed a high rate of discordance in matched pairs of primary tumors and metastases, suggesting that the accurate evaluation of proto-oncogene protein HER-2 (HER2) status is essential before any therapeutic decision. PMID: 30203148
HER2 gene amplification occurred during the early stages of gastric cancer and showed heterogeneity in several cases. HER2 gene amplification may be involved in tumor progression in early gastric cancer. PMID: 30120594
Activating HER2 mutation is present in about 3% of bone metastases from breast cancers, with significantly higher rates in the pleomorphic subtype of lobular cancer. PMID: 30094493
The results suggested a possible link between tRNALeu overexpression and RSK1/MSK2 activation and ErbB2/ErbB3 signaling, especially in breast cancer. PMID: 28816616
High HER2 expression is associated with metastasis in breast Cancer. PMID: 29187405
This study confirmed that biosimilar trastuzumab improves the overall response rate when combined with chemotherapy for HER2+ breast cancer PMID: 30082554
The Authors reveal a gender difference in the prognostic value of concomitant AIB1 and HER2 copy number gain (CNG) in glioma patients which were barely noticed before. These observations indicated that genetic alterations synergistic with essential respects of sex determination influence glioma biology and patients outcomes. PMID: 30153912
The survival rates in this study are equal to the documented global rates; nodal disease burden emerged as the most important prognostic factor. In addition, in EBCs, a lack of hormone receptor expression and in LABC, Her2neu overexpression appear to worsen the outcome. PMID: 30147088
Results showed that HER2 and FGFR2 are regulated by DDX6 at the post-transcriptional step in gastric cancer. PMID: 29987267
HER2 overexpression is associated with Gastric Cancer. PMID: 29938472
ERBB2 oncogene at 17q12 is susceptible to palindromic gene amplification in HER2-positive breast tumors. PMID: 28211519
Results show mutation in ERBB2-exon17 was associated with worse survival results in patients with pancreatic neoplasm. [review] PMID: 30227250
High HER2 expression and Gene Amplification is associated with Upper Tract Urothelial Carcinomas. PMID: 28755093
High HER2 expression is associated with invasion and lymph node metastasis in gastric cancer. PMID: 29970682
Basal HER2 phenotype showed poor DFS, but equivalent pCR rate after concurrent neo-adjuvant chemotherapy with trastuzumab. A different treatment approach to basal-HER2 type is needed even for cases that achieved adequate clinical response after neo-adjuvant chemotherapy. PMID: 29971625
In the largest series reported to date, patients with HER2-amplified m17 cancers treated with trastuzumab have outcomes comparable to patients from the large phase III adjuvant trastuzumab trials who were HER2-positive, supporting the critical role of HER2-directed therapy in this patient population. PMID: 28986743
The interplay of dual MET/HER2 overexpression in the AKT and ERK pathways for esophageal cancer is described. Therefore, combination therapy could be a novel strategy for EAC with amplification of both MET and HER2. PMID: 29223420
Study provides evidence that the hostile environment developed in spheroids has a key role in the acquisition of resistance to Trastuzumab and is associated with an increase in the number of breast cancer stem cells as well as a modulation in HER2 expression. PMID: 28722778
A major finding of our study is that one out of five (20%) patients with breast cancer BM had a receptor discrepancy between the primary tumor and the subsequent BM, with loss of hormone receptors (ER and/or PR) expression, and gain of HER2 overexpression as the most commonly observed changes PMID: 28975433
High HER2 expression is associated with Gastric Adenocarcinoma. PMID: 29802704
Absence of HER2 Expression of Circulating Tumor Cells is associated with Non-Metastatic Esophageal Cancer. PMID: 30275185
HER2 positivity was found in a minority of rectal cancer patients and was not significantly associated with clinicopathologic and molecular characteristics. PMID: 30056472
Study discovered a novel enhancer HER2 gene body enhancer (HGE) in the 3' gene body of HER2. The HGE activates promoters 1 and 2 in trans., and hence the TFAP2C-mediated transcriptional induction of HER2 expression in breast cancer samples. PMID: 29035388
ctDNA gene mutation profiles differed among HR/HER2 subtypes of metastatic breast cancer (MBC) patients. By identifying mutations associated with treatment resistance, we hope to improve therapy selection for MBC patients who received multiline treatment. PMID: 29807833
It was concluded that miR494 inhibited the cancer initiating cells phenotype and reversed resistance to lapatinib by inhibiting FGFR2 in HER2positive gastric cancer. PMID: 29786108
HER2 overexpression was evident in nearly 25% of the Malaysian patients with locally advanced or metastatic gastric cancer. The overexpression correlated significantly with male gender and diffuse-type tumors. PMID: 28124769
There was a statistically significant association between positive p95-HER2 expression and negative hormonal receptors expression (p=0.004), high Ki-67 expression (p<0.001) and development of visceral metastasis PMID: 29779938
The authors herein prove, for the first time, that the transcriptional repressor Blimp1 is a novel mediator of p130Cas/ErbB2-mediated invasiveness. Indeed, high Blimp1 expression levels are detected in invasive p130Cas/ErbB2 cells and correlate with metastatic status in human breast cancer patients. PMID: 28442738
ERBB2 amplification is driving resistance to erlotinib in lung adenocarcinoma. PMID: 28870636
Results showed that combining the results of IHC and FISH according to the HER2 testing algorithm is a useful method for accurately evaluating HER2-positive EMPD. PMID: 29744813
Because the concordance rates of HER2 IHC score 2/3+ cases were lower than that of HER2 IHC score 0/1+ cases, further studies for detailed analysis criteria for HER2 IHC score 2+ or 3+ are required. PMID: 28478639
HER2 interacts with Beclin 1 in breast cancer cells and inhibits autophagy. Mice with increased basal autophagy due to a genetically engineered mutation in Becn1 are protected from human HER2-driven mammary tumorigenesis. HER2-mediated inhibition of Beclin 1 and autophagy likely contributes to HER2-mediated tumorigenesis. PMID: 29610308
These findings suggest that early-stage morphological alterations of HER2-positive BC cells during cancer progression can occur in a physical and signalling-independent manner. PMID: 27599456

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Involvement in disease

Glioma (GLM); Ovarian cancer (OC); Lung cancer (LNCR); Gastric cancer (GASC)

Subcellular Location

[Isoform 1]: Cell membrane; Single-pass type I membrane protein. Early endosome. Cytoplasm, perinuclear region. Nucleus.; [Isoform 2]: Cytoplasm. Nucleus.; [Isoform 3]: Cytoplasm. Nucleus.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, EGF receptor subfamily

Tissue Specificity

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

Database Links

HGNC: 3430

OMIM: 137800

KEGG: hsa:2064

STRING: 9606.ENSP00000269571

UniGene: Hs.446352

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Code	CSB-MP007763HU
MSDS	MSDS Datasheet
Size	$138
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized HER2 at 2 μg/ml can bind Trastuzumab, the EC₅₀ is 2.179-2.825 ng/ml. Biological Activity Assay
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Recombinant Human Receptor tyrosine-protein kinase erbB-2 (ERBB2), partial (Active)

Product Details

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Target Background

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