Chicken Interleukin 8(IL-8) ELISA Kit

Code CSB-E14191C
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Chicken IL8 ELISA Kit was designed for the quantitative measurement of Chicken IL8 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.56 pg/mL-400 pg/mL and the sensitivity is 0.78 pg/mL.

Target Name interleukin 8
Alternative Names CXCL8 ELISA Kit; CEF4 ELISA Kit; EMF1 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; 9E3 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; CEF-4 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Embryo fibroblast protein 1 ELISA Kit; EMF-1 ELISA Kit
Abbreviation IL8
Uniprot No. P08317
Species Gallus gallus (Chicken)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-400 pg/mL
Sensitivity 0.78 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %102
Range %97-106
1:2Average %88
Range %84-93
1:4Average %104
Range %99-109
1:8Average %103
Range %99-106
Recovery
The recovery of chicken IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9894-102
EDTA plasma (n=4)9185-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
4000.187 0.183 0.185
1000.259 0.267 0.263
250.471 0.452 0.462
6.250.862 0.843 0.853
1.561.410 1.398 1.404
02.251 2.159 2.205
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

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 Q&A
Q:

Can I get a copy of the kit instructions to as well as any references to papers that have used this kit? I want to make sure it will work in our specific use case. To that end I'm looking for information about the nature of the antibodies (capture and detection) are they monoclonal or poly. Are they the same Ab for both steps or different? Are the plates recoated, or do we need to coat them ourselves? Thank you for the help.

A:
Thanks for your inquiry.
I will send you the manual by e-mail.
Here is the reference about this kit: https://www.sciencedirect.com/science/article/pii/S0165242717302362
The plate is ready to use and you don't need to coat it by yourself. The plate is coated with specific antigen.There is only one antibody in the kit and it is goat polyclonal antibody.
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
May be an autocrine factor that promotes the growth of fibroblasts and is involved in the neoplastic transformation of fibroblasts by v-Src. Chemotactic for peripheral blood mononuclear cells as well as for heterophils.
Gene References into Functions
  1. Lower levels in airway aspirates from breastfed infants with acute bronchiolitis PMID: 20337964
Subcellular Location Secreted.
Protein Families Intercrine alpha (chemokine CxC) family
Database Links

KEGG: gga:396495

STRING: 9031.ENSGALP00000019051

UniGene: Gga.826

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