Chicken Interleukin 8(IL-8) ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Target Background

CloseMessage

Home
Kit
ELISA Kit
Chicken Interleukin 8(IL-8) ELISA Kit

Citations (6) Protocol

Code	CSB-E14191C
Size	96T,5×96T,10×96T
Trial Size
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Citations (6)
Related Products
Customer Reviews and Q&A
Target Background

Description

This Chicken IL8 ELISA Kit was designed for the quantitative measurement of Chicken IL8 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.56 pg/mL-400 pg/mL and the sensitivity is 0.78 pg/mL.

Target Name

interleukin 8

Alternative Names

CXCL8 ELISA Kit; CEF4 ELISA Kit; EMF1 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; 9E3 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; CEF-4 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Embryo fibroblast protein 1 ELISA Kit; EMF-1 ELISA Kit

Abbreviation

IL8

Uniprot No.

P08317

Species

Gallus gallus (Chicken)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.56 pg/mL-400 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	97-106
1:2	Average %	88
	Range %	84-93
1:4	Average %	104
	Range %	99-109
1:8	Average %	103
	Range %	99-106

Recovery

The recovery of chicken IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	91	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
400	0.187	0.183	0.185
100	0.259	0.267	0.263
25	0.471	0.452	0.462
6.25	0.862	0.843	0.853
1.56	1.410	1.398	1.404
0	2.251	2.159	2.205

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Comparative Analysis of the Liver Transcriptome of Beijing You Chickens and Guang Ming Broilers under Salmonella enterica Serovar Typhimurium Infection Z Wang,Microorganisms ,2022
The immunological effect and pharmacological interaction of tylvalosin with lactobacillus acidophilus during vaccination of chickens with NDV vaccine MA Fadel,Advances in Animal and Veterinary Sciences,2021
Clostridium butyricum alone or combined with 1, 25‐dihydroxyvitamin D3 improved early‐stage broiler health by modulating intestinal flora Y Yu,Journal of Applied Microbiology,2021
Enhanced cytokine expression and upregulation of inflammatory signaling pathways in broiler chickens affected by wooden breast myopathy T Xing,J. Sci. Food Agric,2020
Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation Saleh KMM, et al,Animals,2019
Differences in expression of genes in the MyD88 and TRIF signalling pathways and methylation of TLR4 and TRIF in Tibetan chickens and DaHeng S03 chickens infected with Salmonella enterica serovar Enteritidis Xiaocheng Li.et al,Veterinary Immunology and Immunopathology,2017

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

■ Q&A

Can I get a copy of the kit instructions to as well as any references to papers that have used this kit? I want to make sure it will work in our specific use case. To that end I'm looking for information about the nature of the antibodies (capture and detection) are they monoclonal or poly. Are they the same Ab for both steps or different? Are the plates recoated, or do we need to coat them ourselves? Thank you for the help.

Thanks for your inquiry.
I will send you the manual by e-mail.
Here is the reference about this kit: https://www.sciencedirect.com/science/article/pii/S0165242717302362
The plate is ready to use and you don't need to coat it by yourself. The plate is coated with specific antigen.There is only one antibody in the kit and it is goat polyclonal antibody.
Pls let me know if you have any further questions. Thank you.

Function (From Uniprot)	May be an autocrine factor that promotes the growth of fibroblasts and is involved in the neoplastic transformation of fibroblasts by v-Src. Chemotactic for peripheral blood mononuclear cells as well as for heterophils.
Gene References into Functions	Lower levels in airway aspirates from breastfed infants with acute bronchiolitis PMID: 20337964
Subcellular Location	Secreted.
Protein Families	Intercrine alpha (chemokine CxC) family
Database Links	KEGG: gga:396495 STRING: 9031.ENSGALP00000019051 UniGene: Gga.826

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

TWIK2 channel mediates inflammation, study finds Two Types of Macrophages: M1 and M2 Macrophages LPS/TLR4 Signal Transduction Pathway The Overview of Chemokine The Overview of Interleukin ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

Related pathways

Chemokine signaling pathway NF-kappa B signaling pathway Cellular senescence IL-17 signaling pathway NOD-like receptor signaling pathway Toll-like receptor signaling pathway AGE-RAGE signaling pathway in diabetic complications Oxidative Phosphorylation Phospholipase D signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St Ste 610-322 Houston, TX 77054, USA

Join Us with

Subscribe newsletter

Leave a message

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Product >> Product Name：
Product >> Code：
Product >> Size：
Name： *
Email： *
Phone：
We recommend you to leave your phone number because occasionally our emails get flagged by spam filters. We would not disclose any of your private information to any third party.
Question/Comment： *
Your Region： *
Country State：
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
Check Code：