Chicken Interleukin 8(IL-8) ELISA Kit

Product Details

Citations

Q&A and Customer Reviews

Target Data

Most popular with customers

Your Good Partner in Biology Research

Tel : 301-363-4651

Home
Kit
ELISA Kit
Chicken Interleukin 8(IL-8) ELISA Kit

^Instructions

Code	CSB-E14191C
Size	96T,5×96T,10×96T
See More Details

Product Details
Citations
Related Products
Q&A and Customer Reviews
Target Data

Target Name

interleukin 8

Alternative Names

CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, T cell chemotactic factor|beta-thromboglobulin-like protein|chemokine (C-X-C motif) ligand 8|emoctakin|granulocyte chemotactic

Abbreviation

IL8

Uniprot No.

P08317

Species

Gallus gallus (Chicken)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.56 pg/mL-400 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	97-106
1:2	Average %	88
	Range %	84-93
1:4	Average %	104
	Range %	99-109
1:8	Average %	103
	Range %	99-106

Recovery

The recovery of chicken IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	91	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
400	0.187	0.183	0.185
100	0.259	0.267	0.263
25	0.471	0.452	0.462
6.25	0.862	0.843	0.853
1.56	1.410	1.398	1.404
0	2.251	2.159	2.205

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Still Have Questions?

Leave a Message or Start an on-line Chat

Differences in expression of genes in the MyD88 and TRIF signalling pathways and methylation of TLR4 and TRIF in Tibetan chickens and DaHeng S03 chickens infected with Salmonella enterica serovar Enteritidis Xiaocheng Li.et al,Veterinary Immunology and Immunopathology,2017
Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation Saleh KMM, et al,Animals,2019

■ Q&A

Can I get a copy of the kit instructions to as well as any references to papers that have used this kit? I want to make sure it will work in our specific use case. To that end I'm looking for information about the nature of the antibodies (capture and detection) are they monoclonal or poly. Are they the same Ab for both steps or different? Are the plates recoated, or do we need to coat them ourselves? Thank you for the help.

Thanks for your inquiry.
I will send you the manual by e-mail.
Here is the reference about this kit: https://www.sciencedirect.com/science/article/pii/S0165242717302362
The plate is ready to use and you don't need to coat it by yourself. The plate is coated with specific antigen.There is only one antibody in the kit and it is goat polyclonal antibody.
Pls let me know if you have any further questions. Thank you.

■ Customer Reviews

Submit CUSABIO Product Reciew in 2 Minutes, Win An Apple Watch!

Function	May be an autocrine factor that promotes the growth of fibroblasts and is involved in the neoplastic transformation of fibroblasts by v-Src. Chemotactic for peripheral blood mononuclear cells as well as for heterophils.
Subcellular Location	Secreted
Protein Families	Intercrine alpha (chemokine CxC) family
Database Links	KEGG: gga:396495 STRING: 9031.ENSGALP00000019051 UniGene: Gga.826

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Human 25-hydroxy vitamin D3,25HVD3 ELISA Kit

Detect Range: 20 μg/L-100 μg/L

Sensitivity: 5 μg/L
Rat 25-hydroxy vitamin D3(25 HVD3)ELISA Kit

Detect Range: 20 μg/L-100 μg/L

Sensitivity: 5 μg/L
Human α2 macroglobulin,α2 MG ELISA Kit

Detect Range: 1.25 μg/mL-20 μg/mL

Sensitivity: 1.25 μg/mL
Human Arachidonic Acid,AA ELISA Kit

Detect Range: 78.125 ng/mL-5000 ng/mL

Sensitivity: 69.748 ng/mL
Rat Arachidonic Acid(AA) ELISA Kit

Detect Range: 3.125 ng/mL-200 ng/mL

Sensitivity: 2.758 ng/mL
Human apolipoprotein E (Apo-E) ELISA Kit

Detect Range: 15.62 ng/mL-1000 ng/mL

Sensitivity: 3.9 ng/mL
Hamster adiponectin, C1Q and collagen domain containing (ADIPOQ)ELISA kit

Detect Range: 0.469 ng/mL-30 ng/mL

Sensitivity: 0.117 ng/mL
Human adiponectin,ADP ELISA Kit

Detect Range: 1.562 ng/mL-100 ng/mL

Sensitivity: 1.102 ng/mL