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| Code | CSB-E14191C |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 97-106 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:4 | Average % | 104 | ||||
| Range % | 99-109 | |||||
| 1:8 | Average % | 103 | ||||
| Range % | 99-106 | |||||
| The recovery of chicken IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 91 | 85-96 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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This Chicken Interleukin 8 (IL-8) ELISA Kit is used for detecting and quantifying IL-8 protein in your chicken samples. IL-8,a chemokine involved in inflammatory responses and infection,plays a crucial role in the immune system. Our ELISA kit is designed specifically for the detection of IL-8 in serum, plasma, and tissue homogenates of Gallus gallus (chicken) samples.
With a detection range of 1.56 pg/mL-400 pg/mL and a sensitivity of 0.78 pg/mL, this ELISA kit offers precise and accurate measurement of IL-8 levels in your samples. The assay principle is quantitative and competitive, making it highly specific and reliable. Assay time ranges from 1-5 hours, depending on the sample volume used (50-100ul), and detection wavelength is set at 450 nm. This kit is ideal for researchers in the field of immunology, as it can help in understanding the role of IL-8 in the immune response of chickens.
The Chicken IL-8 ELISA Kit has been validated and cited in more than 6 research papers, making it a trusted tool for your research needs.
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Can I get a copy of the kit instructions to as well as any references to papers that have used this kit? I want to make sure it will work in our specific use case. To that end I'm looking for information about the nature of the antibodies (capture and detection) are they monoclonal or poly. Are they the same Ab for both steps or different? Are the plates recoated, or do we need to coat them ourselves? Thank you for the help.
