The sensitive quantitative measurement of total human lysophosphatidic acid (LPA) in serum, plasma, and tissue homogenates is easily performed with this 96 well strip format ELISA kit. This assay employs the competitive inhibition enzyme immunoassay technique, in which pre-coated LPA competes with LPA (standards or samples) for the HRP-conjugated antibody specific for LPA. And the color elicited by HRP-TMB chromogenic reaction develops in opposite to the amount of LPA in the sample. The color development is stopped upon the addition of the stop solution and the intensity of the color is measured by a microplate reader at 450 nm.
This Human LPA ELISA Kit has high sensitivity and excellent specificity for detection of LPA. And it also has been validated with precision low than 10%, good linearity, high recovery, and lot-to-lot consistency. Refer to the product instructions for more details.
LPA is a membrane-derived lysophospholipid that can function as a signaling molecule. Upon recognition by GPCRs (LPAR1-LPAR6), LPA mediates in a plethora of biological responses, including differentiation, proliferation, migration, vascular regulation, synaptic plasticity, neurogenesis, cell survival, and cytoskeletal remodeling. Aberrant LPA signaling has been linked to malignant behaviors in various cancers, including breast cancer. Dysregulation of the LPA receptors can lead to hyperproliferation, which may contribute to oncogenesis and metastasis.