BCL2, the B-cell lymphoma/leukaemia-2 gene, aliases include anti-apoptotic proteins in the Bcl-2 family, such as Bcl-2, Bcl-XL, Bcl-W, Mcl-1 and others. It was initially identified in blood lymphocytes and was able to significantly inhibit cell death.The BCL2 gene has a complex mechanism of action and inhibits apoptosis mainly through the regulation of the mitochondrial pathway, where it prevents the release of cytochrome c and inhibits the effects of activated caspases on mitochondria, thereby maintaining cell survival. In addition, BCL2 also acts through various mechanisms such as antioxidant effects, inhibition of calcium ion flow across the membrane and regulation of ion channel protein function.
BCL2 plays a key role in the signalling pathway of apoptosis by interacting with pro-apoptotic proteins, such as Bax, to form a dimer, which regulates mitochondrial membrane permeability, and thus affects the release of cytochrome c and activation of the Caspase cascade reaction. The biological significance of BCL2 is significant, as it not only plays a role in the balance between cell survival and apoptosis, but is also closely related to the development of many diseases, including cancer and neurodegenerative diseases, etc. Dysfunction of BCL2 may lead to abnormal activation of apoptotic pathways, which may affect normal physiological functions of cells.
Recombinant Rat Apoptosis regulator Bcl-2 (Bcl2) (CSB-CF002611RA)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
BCL2 Recombinant Monoclonal Antibody (CSB-RA564360A0HU)
Validated Data
Western Blot
Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U-937 whole cell lysate, THP-1 whole cell lysate, Raji whole cell lysate, Mouse Spleen whole cell lysate
All lanes: Bcl-2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 27, 23 kDa
Observed band size: 27 kDa
IHC image of CSB-RA564360A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Rat B-cell CLL/lymphoma 2 (BCL2) ELISA kit (CSB-E08854r)
Validated Data
Code: CSB-E08854r
Size: 96T,5×96T,10×96T
Sensitivity: 0.078 ng/mL
Detection Range: 0.312 ng/mL-20 ng/mL
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The following BCL2 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
BCL2 Antibodies for Mus musculus (Mouse)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA002611LC01MO | Bcl2 Antibody, FITC conjugated | Mouse | |
CSB-PA002611LA01MO | Bcl2 Antibody | Mouse | ELISA, IHC |
CSB-PA002611LB01MO | Bcl2 Antibody, HRP conjugated | Mouse | ELISA |
CSB-PA006342 | Phospho-BCL2 (S70) Antibody | Mouse,Rat | WB, ELISA |
BCL2 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA002611GA01HU | BCL2 Antibody | Human,Mouse,Rat,Guinea pig | ELISA,WB,IHC |
CSB-PA187601 | BCL2 (Ab-56) Antibody | Human | ELISA,WB,IHC,IF |
CSB-PA129411 | BCL2 (Ab-70) Antibody | Human | ELISA,WB,IHC,IF |
CSB-PA206782 | Phospho-BCL2 (Thr56) Antibody | Human | ELISA,IHC |
CSB-PA824266 | Phospho-BCL2 (Ser70) Antibody | Human | ELISA,IHC |
CSB-PA971921 | Phospho-BCL2 (Ser87) Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-MA000196 | BCL2 Monoclonal Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA000484 | Phospho-BCL2 (S70) Antibody | Human,Monkey | WB, IHC, IP, ELISA |
CSB-PA000485 | Phospho-BCL2 (T56) Antibody | Human | WB, IHC, IP, IF, ELISA |
CSB-PA000982 | BCL2 Antibody | Human,Monkey | WB, IHC, ELISA |
CSB-PA000983 | BCL2 Antibody | Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-PA000984 | BCL2 Antibody | Human | WB, IHC, IF, ELISA |
CSB-PA040190 | Phospho-BCL2 (T69) Antibody | Human | IHC, IF, ELISA |
CSB-PA040191 | Phospho-BCL2 (S87) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040192 | Phospho-BCL2 (T74) Antibody | Human | IHC, ELISA |
CSB-PA597947 | BCL2 Antibody | Human | ELISA,WB |
CSB-PA832691 | BCL2 Antibody | Human | ELISA,WB,IHC |
CSB-PA703826 | BCL2 Antibody | Mouse,Rat | WB,ELISA |
CSB-PA12959A0Rb | BCL2 Antibody | Human | ELISA, IHC, IF |
CSB-PA12959B0Rb | BCL2 Antibody, HRP conjugated | Human | ELISA |
CSB-PA12959C0Rb | BCL2 Antibody, FITC conjugated | Human | |
CSB-PA12959D0Rb | BCL2 Antibody, Biotin conjugated | Human | ELISA |
CSB-RA002611A0HU | BCL2 Recombinant Monoclonal Antibody | Human, Mouse, Rat | ELISA, WB, IHC, FC |
CSB-RA564360A0HU | BCL2 Recombinant Monoclonal Antibody | Human, Mouse | ELISA, WB, IHC |
CSB-MA002611A0m | BCL2 Monoclonal Antibody | Human, Mouse | ELISA, WB, FC |
BCL2 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP002611MO CSB-BP002611MO CSB-MP002611MO CSB-EP002611MO-B |
Recombinant Mouse Apoptosis regulator Bcl-2 (Bcl2), partial | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-CF002611MO(A4) | Recombinant Mouse Apoptosis regulator Bcl-2 (Bcl2) | in vitro E.coli expression system |
CSB-EP002611MO | Recombinant Mouse Apoptosis regulator Bcl-2 (Bcl2), partial | E.coli |
BCL2 Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-CF002611BO | Recombinant Bovine Apoptosis regulator Bcl-2 (BCL2) | in vitro E.coli expression system |
CSB-YP002611BO1 CSB-EP002611BO1 CSB-BP002611BO1 CSB-MP002611BO1 CSB-EP002611BO1-B |
Recombinant Bovine Apoptosis regulator Bcl-2 (BCL2), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
BCL2 Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-CF002611CH | Recombinant Chicken Apoptosis regulator Bcl-2 (BCL2) | in vitro E.coli expression system |
CSB-YP002611CH1 CSB-EP002611CH1 CSB-BP002611CH1 CSB-MP002611CH1 CSB-EP002611CH1-B |
Recombinant Chicken Apoptosis regulator Bcl-2 (BCL2), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
BCL2 Proteins for Canis lupus familiaris (Dog) (Canis familiaris)
Code | Product Name | Source |
---|---|---|
CSB-CF744176DO | Recombinant Dog Apoptosis regulator Bcl-2 (BCL2) | in vitro E.coli expression system |
CSB-YP744176DO1 CSB-EP744176DO1 CSB-BP744176DO1 CSB-MP744176DO1 CSB-EP744176DO1-B |
Recombinant Dog Apoptosis regulator Bcl-2 (BCL2), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
BCL2 Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Code | Product Name | Source |
---|---|---|
CSB-CF872860DXU | Recombinant Cricetulus griseus Apoptosis regulator Bcl-2 (BCL2) | in vitro E.coli expression system |
CSB-YP872860DXU1 CSB-EP872860DXU1 CSB-BP872860DXU1 CSB-MP872860DXU1 CSB-EP872860DXU1-B |
Recombinant Cricetulus griseus Apoptosis regulator Bcl-2 (BCL2), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
BCL2 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-CF002611RA | Recombinant Rat Apoptosis regulator Bcl-2 (Bcl2) | in vitro E.coli expression system |
BCL2 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-CF002611HU | Recombinant Human Apoptosis regulator Bcl-2 (BCL2) | in vitro E.coli expression system |
CSB-EP002611HU1 | Recombinant Human Apoptosis regulator Bcl-2 (BCL2), partial | E.coli |
CSB-YP002611HU1 CSB-BP002611HU1 CSB-MP002611HU1 CSB-EP002611HU1-B |
Recombinant Human Apoptosis regulator Bcl-2 (BCL2), partial | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
BCL2 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP002611RA1 CSB-EP002611RA1 CSB-BP002611RA1 CSB-MP002611RA1 CSB-EP002611RA1-B |
Recombinant Rat Apoptosis regulator Bcl-2 (Bcl2), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
BCL2 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E08853h | Human B-cell leukemia/lymphoma 2,Bcl-2 ELISA Kit | serum, plasma, tissue homogenates | 0.225 ng/mL |
BCL2 ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E08855m | Mouse B-cell leukemia/lymphoma 2,Bcl-2 ELISA Kit | serum, plasma | 3.9 pg/mL |
BCL2 ELISA Kit for Rattus norvegicus (Rat)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E08854r | Rat B-cell CLL/lymphoma 2 (BCL2) ELISA kit | serum, plasma, tissue homogenates | 0.078 ng/mL |