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The BCL2 recombinant monoclonal antibody is produced through a complex series of steps. The process begins with the harvesting of the BCL2 monoclonal antibody and sequencing of its genetic sequence. Next, a vector carrying the BCL2 monoclonal antibody gene is created and transfected into a host cell line for culturing. A synthesized peptide derived from human BCL2 is used to stimulate the BCL2 monoclonal antibody production. The BCL2 recombinant monoclonal antibody is purified using affinity chromatography to achieve high specificity and purity. Finally, the specificity of the antibody is confirmed using ELISA, WB, and IHC assays to verify its ability to detect BCL2. It reacts with human and mouse BCL2 proteins.
The BCL2 protein plays a key role in regulating apoptosis, which is a programmed cell death process that is essential for normal development and homeostasis in multicellular organisms. BCL2 is an anti-apoptotic protein that promotes cell survival by inhibiting the pro-apoptotic members of the family. BCL2 also plays a role in the regulation of mitochondrial function. BCL2 can interact with and modulate the activity of several mitochondrial proteins, including the voltage-dependent anion channel (VDAC), which regulates mitochondrial outer membrane permeability, and the adenine nucleotide translocator (ANT), which is involved in mitochondrial energy metabolism. It also has a role in the regulation of the cell cycle. Dysregulation of BCL2 expression or function is associated with cancer and resistance to chemotherapy.
Applications : Western blots
Sample type: cells
Review: Expression of apoptotic proteins with respective positive bands at 38 kDa for Caspase 12, 38 kDa for Mcl 1, 26 kDa for BAX, 26 kDa for Bcl 2, 36 kDa for cyt c, 60 kDa for PARP3, Dox—Doxorubicin, S1—NC4, S2—NC5, S3—NC6, S4—NP1, S5—NP6 Dox, S6—Chit-ND1.