NUP98

What is NUP98?

The NUP98 gene belongs to the nucleoporin gene family. It is exclusively expressed in the vertebrate. Due to alternative mRNA splicing, human NUP98 commonly exists in two forms. One transcript codes for a 98 kDa nucleoporin named NUP98, which can be posttranslationally cleaved into a 90 kDa N-terminal fragment and an 8 kDa C-terminal fragment. The other encodes a NUP98-NUP96 precursor protein that is posttranslationally cleaved after Phe863 to form the 90 kDa N-terminal protein (hNUP98) and the C-terminal hNUP96.

What is the feature of the NUP98 structure?

The human NUP98 polypeptide chain contains two phenylalanine-glycine (FG)-repeat regions, a Gle2-binding sequence (GLEBS) motif lying between the two FG-repeat regions, and an RNA-binding motif. The FG-repeats provide multiple binding sites for nuclear transport factors as they carry cargo through the nuclear pore complex (NPC). The GLEBS motif interacts with the ribonucleic acid export 1 (RAE1), an mRNA binding protein linked to mRNA export, to form the RAE1-NUP98 complex capable of binding single-stranded RNA. The alone expressed 90 kDa N-terminal fragment localizes to the NPC, suggesting that localization to the NPC is somehow blocked by the uncleaved C-terminal fragment.

What is the function of the NUP98?

SARS-CoV Orf6 protein was previously shown to sequester nuclear import factors on the ER/Golgi membrane, thus blocking the expression of genes that establish antiviral response. However, SARS-CoV2 Orf6 was identified to interact with NUP98 and its interaction partner RAE1, probably preventing cellular mRNA export.

Additionally, NUP98 plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Human NUP98 is involved in many cellular processes, including nuclear bidirectional transport, mitotic progression, and regulation of gene expression. And it may anchor NUP153 and TPR to the NPC. NUP98 binds DHX9 and regulates its RNA-dependent ATPase activity. And translocations between the NUP98 gene and many other partner genes have been observed in different leukemias.

Hot products of NUP98:

Recombinant Human Nuclear pore complex protein Nup98-Nup96(NUP98), partial (C-terminal hFC-tagged) (CSB-MP016209HU)

NUP98 Antibody (ELISA, WB, IF, IP) (CSB-PA016209LA01HU)