RAF1, also recognized as MAPK 1 or CRAF, is a protein kinase encoded by the RAF1 gene and part of the RAF family, with widespread expression across various cell types. Acting as a crucial regulator in the MAPK signaling pathway, RAF1 phosphorylates and activates MEK1/2, leading to the activation of ERK1/2 and influencing cell proliferation, differentiation, migration, and survival. RAF1's role in cell cycle control, stress response, and apoptosis makes it a significant factor in various cellular processes. Its dysregulation is linked to numerous diseases, particularly cancer, where RAF1 mutations or overactivation can result in uncontrolled cell proliferation and drug resistance, highlighting its potential as a therapeutic target.
Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) (CSB-EP019284HUc7)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Phospho-RAF1 (S621) Recombinant Monoclonal Antibody (CSB-RA019284A621phHU)
Validated Data
Western Blot
Positive WB detected in Hela whole cell lysate(treated with Calyculin A or EGF)
All lanes Phospho-RAF1 antibody at 1.525μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73 KDa
Observed band size: 73 KDa
Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with CSB-RA019284A621phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
The following RAF1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
RAF1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA019284LA01HU | RAF1 Antibody | Human | ELISA, WB, IHC, IF, IP |
CSB-PA242762 | RAF1 (Ab-259) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA291059 | Phospho-RAF1 (Ser259) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA151308 | Phospho-RAF1 (Ser338) Antibody | Human,Mouse | ELISA,WB |
CSB-PA209480 | RAF1 (Ab-338) Antibody | Human,Mouse | ELISA,WB,IHC |
CSB-PA445340 | Phospho-RAF1 (Ser621) Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA580751 | Phospho-RAF1 (Tyr341) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA299750 | Phospho-RAF1 (Thr269) Antibody | Human,Mouse | ELISA,IHC |
CSB-PA148049 | Phospho-RAF1 (Ser642) Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA948259 | Phospho-RAF1 (Ser301) Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA000615 | Phospho-RAF1 (S259) Antibody | Human,Mouse,Rat,Monkey | WB, IHC, ELISA |
CSB-PA000660 | Phospho-RAF1 (S338) Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA003921 | RAF1 Antibody | Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-PA003922 | RAF1 Antibody | Human,Mouse,Rat,Monkey | WB, IF, ELISA |
CSB-PA003923 | RAF1 Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA003926 | RAF1 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040148 | Phospho-RAF1 (S43) Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA040149 | Phospho-RAF1 (S301) Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA040150 | Phospho-RAF1 (S642) Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA040151 | Phospho-RAF1 (Y341) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040152 | Phospho-RAF1 (S296) Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040153 | Phospho-RAF1 (S621) Antibody | Human,Mouse,Rat,Monkey | WB, IHC, IF, ELISA |
CSB-PA040154 | Phospho-RAF1 (T269) Antibody | Human,Mouse,Rat | IHC, ELISA |
CSB-PA040155 | Phospho-RAF1 (S289) Antibody | Human,Mouse,Rat | IHC, ELISA |
CSB-PA040156 | RAF1 Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA040157 | RAF1 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040158 | RAF1 Antibody | Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040160 | RAF1 Antibody | Human,Mouse,Rat | IHC, ELISA |
CSB-PA080220 | RAF1 Antibody | Human | WB, IHC |
CSB-PA010547 | RAF1 Antibody | Human,Mouse,Rat | WB, ELISA |
CSB-PA694669 | RAF1 Antibody | Human,Mouse,Rat | ELISA,IHC |
CSB-RA019284A621phHU | Phospho-RAF1 (S621) Recombinant Monoclonal Antibody | Human | ELISA, WB, IF |
CSB-RA019284A259phHU | Phospho-RAF1 (S259) Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC, IF |
CSB-RA019284A43phHU | Phospho-RAF1 (S43) Recombinant Monoclonal Antibody | Human | ELISA, IF |
RAF1 Antibodies for Rattus norvegicus (Rat)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA950764 | Raf1 Antibody | Rat | ELISA,WB |
RAF1 Antibodies for Zea mays (Maize)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA184121XA01ZAX | RAF1 Antibody | Zea mays (Maize) | ELISA, WB (ensure identification of antigen) |
RAF1 Antibodies for Oryza sativa subsp. japonica (Rice)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA269806XA01OFG | RAF1 Antibody | Oryza sativa subsp. japonica (Rice) | ELISA, WB (ensure identification of antigen) |
RAF1 Antibodies for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA553776XA01SVG | RAF1 Antibody | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ELISA, WB (ensure identification of antigen) |
RAF1 Antibodies for Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA524907XA01SXV | raf1 Antibody | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) | ELISA, WB (ensure identification of antigen) |
RAF1 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP019284RA CSB-EP019284RA CSB-BP019284RA CSB-MP019284RA CSB-EP019284RA-B |
Recombinant Rat RAF proto-oncogene serine/threonine-protein kinase (Raf1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP019284HU CSB-BP019284HU CSB-MP019284HU CSB-EP019284HU-B |
Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP019284HU | Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) | E.coli |
CSB-EP019284HUc7 | Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) | E.coli |
RAF1 Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-YP019284CH CSB-EP019284CH CSB-BP019284CH CSB-MP019284CH CSB-EP019284CH-B |
Recombinant Chicken RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP019284BO CSB-EP019284BO CSB-BP019284BO CSB-MP019284BO CSB-EP019284BO-B |
Recombinant Bovine RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Xenopus laevis (African clawed frog)
Code | Product Name | Source |
---|---|---|
CSB-YP019284XBE CSB-EP019284XBE CSB-BP019284XBE CSB-MP019284XBE CSB-EP019284XBE-B |
Recombinant Xenopus laevis RAF proto-oncogene serine/threonine-protein kinase (raf1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Code | Product Name | Source |
---|---|---|
CSB-YP524907SXV CSB-EP524907SXV CSB-BP524907SXV CSB-MP524907SXV CSB-EP524907SXV-B |
Recombinant Schizosaccharomyces pombe Rik1-associated factor 1 (raf1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Code | Product Name | Source |
---|---|---|
CSB-YP722278PYX CSB-EP722278PYX CSB-BP722278PYX CSB-MP722278PYX CSB-EP722278PYX-B |
Recombinant Pongo abelii RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP859572MO CSB-EP859572MO CSB-BP859572MO CSB-MP859572MO CSB-EP859572MO-B |
Recombinant Mouse RAF proto-oncogene serine/threonine-protein kinase (Raf1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |