RAF proto-oncogene serine/threonine-protein kinase is a protein in humans that is encoded by RAF1 gene. Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2).
The following RAF1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
RAF1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA019284LA01HU | RAF1 Antibody |
Human | ELISA, WB, IHC, IF, IP |
CSB-PA242762 | RAF1 (Ab-259) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA291059 | Phospho-RAF1 (Ser259) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA151308 | Phospho-RAF1 (Ser338) Antibody |
Human,Mouse | ELISA,WB |
CSB-PA209480 | RAF1 (Ab-338) Antibody |
Human,Mouse | ELISA,WB,IHC |
CSB-PA445340 | Phospho-RAF1 (Ser621) Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA580751 | Phospho-RAF1 (Tyr341) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA299750 | Phospho-RAF1 (Thr269) Antibody |
Human,Mouse | ELISA,IHC |
CSB-PA148049 | Phospho-RAF1 (Ser642) Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA948259 | Phospho-RAF1 (Ser301) Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA000615 | Phospho-RAF1 (S259) Antibody |
Human,Mouse,Rat,Monkey | WB, IHC, ELISA |
CSB-PA000660 | Phospho-RAF1 (S338) Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA003921 | RAF1 Antibody |
Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-PA003922 | RAF1 Antibody |
Human,Mouse,Rat,Monkey | WB, IF, ELISA |
CSB-PA003923 | RAF1 Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA003926 | RAF1 Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040148 | Phospho-RAF1 (S43) Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA040149 | Phospho-RAF1 (S301) Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA040150 | Phospho-RAF1 (S642) Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA040151 | Phospho-RAF1 (Y341) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040152 | Phospho-RAF1 (S296) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040153 | Phospho-RAF1 (S621) Antibody |
Human,Mouse,Rat,Monkey | WB, IHC, IF, ELISA |
CSB-PA040154 | Phospho-RAF1 (T269) Antibody |
Human,Mouse,Rat | IHC, ELISA |
CSB-PA040155 | Phospho-RAF1 (S289) Antibody |
Human,Mouse,Rat | IHC, ELISA |
CSB-PA040156 | RAF1 Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA040157 | RAF1 Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040158 | RAF1 Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040160 | RAF1 Antibody |
Human,Mouse,Rat | IHC, ELISA |
CSB-PA080220 | RAF1 Antibody |
Human | WB, IHC |
CSB-PA010547 | RAF1 Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA694669 | RAF1 Antibody |
Human,Mouse,Rat | ELISA,IHC |
CSB-RA019284A621phHU | Phospho-RAF1 (S621) Recombinant Monoclonal Antibody |
Human | ELISA, WB, IF |
CSB-RA019284A259phHU | Phospho-RAF1 (S259) Recombinant Monoclonal Antibody |
Human | ELISA, WB, IHC, IF |
CSB-RA019284A43phHU | Phospho-RAF1 (S43) Recombinant Monoclonal Antibody |
Human | ELISA, IF |
RAF1 Antibodies for Rattus norvegicus (Rat)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA950764 | Raf1 Antibody |
Rat | ELISA,WB |
RAF1 Antibodies for Zea mays (Maize)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA184121XA01ZAX | RAF1 Antibody |
Zea mays (Maize) | ELISA, WB (ensure identification of antigen) |
RAF1 Antibodies for Oryza sativa subsp. japonica (Rice)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA269806XA01OFG | RAF1 Antibody |
Oryza sativa subsp. japonica (Rice) | ELISA, WB (ensure identification of antigen) |
RAF1 Antibodies for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA553776XA01SVG | RAF1 Antibody |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ELISA, WB (ensure identification of antigen) |
RAF1 Antibodies for Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA524907XA01SXV | raf1 Antibody |
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) | ELISA, WB (ensure identification of antigen) |
RAF1 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP019284RA CSB-EP019284RA CSB-BP019284RA CSB-MP019284RA CSB-EP019284RA-B |
Recombinant Rat RAF proto-oncogene serine/threonine-protein kinase (Raf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP019284HU CSB-BP019284HU CSB-MP019284HU CSB-EP019284HU-B |
Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP019284HU | Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) |
E.coli |
RAF1 Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-YP019284CH CSB-EP019284CH CSB-BP019284CH CSB-MP019284CH CSB-EP019284CH-B |
Recombinant Chicken RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP019284BO CSB-EP019284BO CSB-BP019284BO CSB-MP019284BO CSB-EP019284BO-B |
Recombinant Bovine RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Xenopus laevis (African clawed frog)
Code | Product Name | Source |
---|---|---|
CSB-YP019284XBE CSB-EP019284XBE CSB-BP019284XBE CSB-MP019284XBE CSB-EP019284XBE-B |
Recombinant Xenopus laevis RAF proto-oncogene serine/threonine-protein kinase (raf1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Code | Product Name | Source |
---|---|---|
CSB-YP524907SXV CSB-EP524907SXV CSB-BP524907SXV CSB-MP524907SXV CSB-EP524907SXV-B |
Recombinant Schizosaccharomyces pombe Rik1-associated factor 1 (raf1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Code | Product Name | Source |
---|---|---|
CSB-YP722278PYX CSB-EP722278PYX CSB-BP722278PYX CSB-MP722278PYX CSB-EP722278PYX-B |
Recombinant Pongo abelii RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP859572MO CSB-EP859572MO CSB-BP859572MO CSB-MP859572MO CSB-EP859572MO-B |
Recombinant Mouse RAF proto-oncogene serine/threonine-protein kinase (Raf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF proto-oncogene serine/threonine-protein kinase is a protein in humans that is encoded by RAF1 gene [1][2]. RAF1 is part of a signaling pathway called the RAS/MAPK pathway, which transmits chemical signals from outside the cell to the cell's nucleus. RAS/MAPK signaling helps control the growth and division (proliferation) of cells, the process by which cells mature to carry out specific functions (differentiation), cell movement (migration), and the self-destruction of cells (apoptosis). As a serine/threonine-protein kinase, RAF1 acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2).
[1] Li P, Wood K, Mamon H, Haser W, Roberts T (February 1991). "Raf-1: a kinase currently without a cause but not lacking in effects". Cell. 64 (3): 479–82.
[2] Rapp UR, Goldsborough MD, Mark GE, Bonner TI, Groffen J, Reynolds FH, Stephenson JR (July 1983). "Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus". Proc. Natl. Acad. Sci. U.S.A. 80 (14): 4218–22.