RAF1 is a member of the Raf family. The protein encoded by RAF1 is a kinase with multiple functional domains, including a kinase domain and multiple binding sites. The RAF1 protein is involved in the Ras-Raf-MEK-ERK signaling pathway, regulating the signaling of various growth factors within cells, affecting cell proliferation, differentiation, and survival. By activating downstream signaling molecules like MEK and ERK, RAF1 participates in regulating processes such as gene expression, cell cycle, and proliferation. Understanding the RAF1 signaling pathway is of significant importance for the development and research of cancer treatment strategies. Research also indicates that certain drugs can treat specific types of cancer by modulating the RAF1 signaling pathway.
Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
The following RAF1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
RAF1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA019284LA01HU | RAF1 Antibody |
Human | ELISA, WB, IHC, IF, IP |
CSB-PA242762 | RAF1 (Ab-259) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA291059 | Phospho-RAF1 (Ser259) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA151308 | Phospho-RAF1 (Ser338) Antibody |
Human,Mouse | ELISA,WB |
CSB-PA209480 | RAF1 (Ab-338) Antibody |
Human,Mouse | ELISA,WB,IHC |
CSB-PA445340 | Phospho-RAF1 (Ser621) Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA580751 | Phospho-RAF1 (Tyr341) Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA299750 | Phospho-RAF1 (Thr269) Antibody |
Human,Mouse | ELISA,IHC |
CSB-PA148049 | Phospho-RAF1 (Ser642) Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA948259 | Phospho-RAF1 (Ser301) Antibody |
Human,Mouse,Rat | ELISA,WB |
CSB-PA000615 | Phospho-RAF1 (S259) Antibody |
Human,Mouse,Rat,Monkey | WB, IHC, ELISA |
CSB-PA000660 | Phospho-RAF1 (S338) Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA003921 | RAF1 Antibody |
Human,Mouse,Rat | WB, IHC, IF, ELISA |
CSB-PA003922 | RAF1 Antibody |
Human,Mouse,Rat,Monkey | WB, IF, ELISA |
CSB-PA003923 | RAF1 Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA003926 | RAF1 Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040148 | Phospho-RAF1 (S43) Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA040149 | Phospho-RAF1 (S301) Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA040150 | Phospho-RAF1 (S642) Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA040151 | Phospho-RAF1 (Y341) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040152 | Phospho-RAF1 (S296) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040153 | Phospho-RAF1 (S621) Antibody |
Human,Mouse,Rat,Monkey | WB, IHC, IF, ELISA |
CSB-PA040154 | Phospho-RAF1 (T269) Antibody |
Human,Mouse,Rat | IHC, ELISA |
CSB-PA040155 | Phospho-RAF1 (S289) Antibody |
Human,Mouse,Rat | IHC, ELISA |
CSB-PA040156 | RAF1 Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA040157 | RAF1 Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040158 | RAF1 Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
CSB-PA040160 | RAF1 Antibody |
Human,Mouse,Rat | IHC, ELISA |
CSB-PA080220 | RAF1 Antibody |
Human | WB, IHC |
CSB-PA010547 | RAF1 Antibody |
Human,Mouse,Rat | WB, ELISA |
CSB-PA694669 | RAF1 Antibody |
Human,Mouse,Rat | ELISA,IHC |
CSB-RA019284A621phHU | Phospho-RAF1 (S621) Recombinant Monoclonal Antibody |
Human | ELISA, WB, IF |
CSB-RA019284A259phHU | Phospho-RAF1 (S259) Recombinant Monoclonal Antibody |
Human | ELISA, WB, IHC, IF |
CSB-RA019284A43phHU | Phospho-RAF1 (S43) Recombinant Monoclonal Antibody |
Human | ELISA, IF |
RAF1 Antibodies for Rattus norvegicus (Rat)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA950764 | Raf1 Antibody |
Rat | ELISA,WB |
RAF1 Antibodies for Zea mays (Maize)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA184121XA01ZAX | RAF1 Antibody |
Zea mays (Maize) | ELISA, WB (ensure identification of antigen) |
RAF1 Antibodies for Oryza sativa subsp. japonica (Rice)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA269806XA01OFG | RAF1 Antibody |
Oryza sativa subsp. japonica (Rice) | ELISA, WB (ensure identification of antigen) |
RAF1 Antibodies for Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA553776XA01SVG | RAF1 Antibody |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ELISA, WB (ensure identification of antigen) |
RAF1 Antibodies for Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA524907XA01SXV | raf1 Antibody |
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) | ELISA, WB (ensure identification of antigen) |
RAF1 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP019284RA CSB-EP019284RA CSB-BP019284RA CSB-MP019284RA CSB-EP019284RA-B |
Recombinant Rat RAF proto-oncogene serine/threonine-protein kinase (Raf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP019284HU CSB-BP019284HU CSB-MP019284HU CSB-EP019284HU-B |
Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP019284HU | Recombinant Human RAF proto-oncogene serine/threonine-protein kinase (RAF1) |
E.coli |
RAF1 Proteins for Gallus gallus (Chicken)
Code | Product Name | Source |
---|---|---|
CSB-YP019284CH CSB-EP019284CH CSB-BP019284CH CSB-MP019284CH CSB-EP019284CH-B |
Recombinant Chicken RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-YP019284BO CSB-EP019284BO CSB-BP019284BO CSB-MP019284BO CSB-EP019284BO-B |
Recombinant Bovine RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Xenopus laevis (African clawed frog)
Code | Product Name | Source |
---|---|---|
CSB-YP019284XBE CSB-EP019284XBE CSB-BP019284XBE CSB-MP019284XBE CSB-EP019284XBE-B |
Recombinant Xenopus laevis RAF proto-oncogene serine/threonine-protein kinase (raf1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Code | Product Name | Source |
---|---|---|
CSB-YP524907SXV CSB-EP524907SXV CSB-BP524907SXV CSB-MP524907SXV CSB-EP524907SXV-B |
Recombinant Schizosaccharomyces pombe Rik1-associated factor 1 (raf1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Code | Product Name | Source |
---|---|---|
CSB-YP722278PYX CSB-EP722278PYX CSB-BP722278PYX CSB-MP722278PYX CSB-EP722278PYX-B |
Recombinant Pongo abelii RAF proto-oncogene serine/threonine-protein kinase (RAF1), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF1 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP859572MO CSB-EP859572MO CSB-BP859572MO CSB-MP859572MO CSB-EP859572MO-B |
Recombinant Mouse RAF proto-oncogene serine/threonine-protein kinase (Raf1) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
RAF proto-oncogene serine/threonine-protein kinase is a protein in humans that is encoded by RAF1 gene [1][2]. RAF1 is part of a signaling pathway called the RAS/MAPK pathway, which transmits chemical signals from outside the cell to the cell's nucleus. RAS/MAPK signaling helps control the growth and division (proliferation) of cells, the process by which cells mature to carry out specific functions (differentiation), cell movement (migration), and the self-destruction of cells (apoptosis). As a serine/threonine-protein kinase, RAF1 acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2).
[1] Li P, Wood K, Mamon H, Haser W, Roberts T (February 1991). "Raf-1: a kinase currently without a cause but not lacking in effects". Cell. 64 (3): 479–82.
[2] Rapp UR, Goldsborough MD, Mark GE, Bonner TI, Groffen J, Reynolds FH, Stephenson JR (July 1983). "Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus". Proc. Natl. Acad. Sci. U.S.A. 80 (14): 4218–22.