Your Good Partner in Biology Research
Tel : 301-363-4651
Your Good Partner in Biology Research
| Code | CSB-PA445340 |
| Size | US$360 |
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| Full Product Name | Rabbit anti-Homo sapiens (Human) RAF1 Polyclonal antibody | ||||
| Uniprot No. | P04049 | ||||
| Target Names | RAF1 | ||||
| Alternative Names | NS5, CRAF, Raf-1, c-Raf | ||||
| Raised in | Rabbit | ||||
| Species Reactivity | Human,Mouse,Rat | ||||
| Immunogen | Peptide sequence around phosphorylation site of Serine 621(S-A-S(p)-E-P) derived from Human C-RAF. | ||||
| Immunogen Species | Homo sapiens (Human) | ||||
| Clonality | Polyclonal | ||||
| Purification Method | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi | ||||
| Concentration | It differs from different batches. Please contact us to confirm it. | ||||
| Form | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | ||||
| Tested Applications | ELISA,WB | ||||
| Recommended Dilution |
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| Protocols | ELISA Protocol Western Blotting(WB) Protocol |
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| Troubleshooting and FAQs | Antibody FAQs | ||||
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. | ||||
| Still Have Questions? | Leave a Message or Start an on-line Chat |
| Function | Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases |
| Involvement in disease | Noonan syndrome 5 (NS5); LEOPARD syndrome 2 (LPRD2); Cardiomyopathy, dilated 1NN (CMD1NN) |
| Subcellular Location | Cytoplasm, Cell membrane, Mitochondrion, Nucleus |
| Protein Families | Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily |
| Tissue Specificity | In skeletal muscle, isoform 1 is more abundant than isoform 2. |
| Database Links |
HGNC: 9829 OMIM: 164760 KEGG: hsa:5894 STRING: 9606.ENSP00000251849 UniGene: Hs.159130 |
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Tel: 301-363-4651
Email: support@cusabio.com
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