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| Code | CSB-PA445340 |
| Product Type | Polyclonal Antibody |
| Size | US$360 |
| Uniprot No. | P04049 |
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| Immunogen | Peptide sequence around phosphorylation site of Serine 621(S-A-S(p)-E-P) derived from Human C-RAF. |
| Raised in | Rabbit |
| Species Reactivity | Human,Mouse,Rat |
| Specificity | The antibody detects endogenous level of C-RAF only when phosphorylated at Serine 621. |
| Tested Applications | ELISA,WB;WB:1:500-1:1000 |
| Relevance | Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. |
| Form | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi |
| Clonality | Polyclonal |
| Alias | NS5, CRAF, Raf-1, c-Raf |
| Immunogen Species | Homo sapiens (Human) |
| Protocols | ELISA Protocol Western Blotting(WB) Protocol |
| Target Names | RAF1 |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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| Function | Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases |
| Involvement in disease | Noonan syndrome 5 (NS5); LEOPARD syndrome 2 (LPRD2); Cardiomyopathy, dilated 1NN (CMD1NN) |
| Subcellular Location | Cytoplasm, Cell membrane, Mitochondrion, Nucleus |
| Protein Families | Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily |
| Tissue Specificity | In skeletal muscle, isoform 1 is more abundant than isoform 2. |
| Database Links |
HGNC: 9829 OMIM: 164760 KEGG: hsa:5894 STRING: 9606.ENSP00000251849 UniGene: Hs.159130 |
| Pathway |
cAMP signaling pathway
Chemokine signaling pathway ErbB signaling pathway Estrogen signaling pathway Jak-STAT signaling pathway MAPK signaling pathway Melanogenesis mTOR signaling pathway PI3K-Akt signaling pathway VEGF signaling pathway Focal adhesion Gap junction Regulation of actin cytoskeleton Vascular smooth muscle contraction Apoptosis Autophagy Cellular senescence MAPK Erk pathway Ras signaling pathway Signaling pathways regulating pluripotency of stem cells B cell receptor signaling pathway Fc epsilon RI signaling pathway Fc gamma R-mediated phagocytosis Natural killer cell mediated cytotoxicity T cell receptor signaling pathway Apelin signaling pathway cGMP-PKG signaling pathway FoxO signaling pathway Insulin signaling pathway Oxidative Phosphorylation Phospholipase D signaling pathway Rap1 signaling pathway Sphingolipid signaling pathway Excitatory synapse pathway Neurotrophin signaling pathway |
RAF1 Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA019284KA01HU | RAF1 Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-RA019284A259phHU | Phospho-RAF1 (S259) Antibody | Human | ELISA, WB, IHC, IF |
| CSB-PA242762 | RAF1 (Ab-259) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA291059 | Phospho-RAF1 (Ser259) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA151308 | Phospho-RAF1 (Ser338) Antibody | Human,Mouse | ELISA,WB |
| CSB-PA209480 | RAF1 (Ab-338) Antibody | Human,Mouse | ELISA,WB,IHC |
| CSB-PA580751 | Phospho-RAF1 (Tyr341) Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA299750 | Phospho-RAF1 (Thr269) Antibody | Human,Mouse | ELISA,IHC |
| CSB-PA148049 | Phospho-RAF1 (Ser642) Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA948259 | Phospho-RAF1 (Ser301) Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA000615 | Phospho-RAF1 (S259) Antibody | Human,Mouse,Rat,Monkey | WB,IHC,ELISA |
| CSB-PA000660 | Phospho-RAF1 (S338) Antibody | Human,Mouse,Rat | WB,ELISA |
| CSB-PA003921 | RAF1 Antibody | Human,Mouse,Rat | WB,IHC,IF,ELISA |
| CSB-PA003922 | RAF1 Antibody | Human,Mouse,Rat,Monkey | WB,IF,ELISA |
| CSB-PA003923 | RAF1 Antibody | Human,Mouse,Rat | WB,ELISA |
| CSB-PA003926 | RAF1 Antibody | Human,Mouse,Rat | WB,IHC,ELISA |
| CSB-PA040148 | Phospho-RAF1 (S43) Antibody | Human,Mouse,Rat | WB,ELISA |
| CSB-PA040149 | Phospho-RAF1 (S301) Antibody | Human,Mouse,Rat | WB,ELISA |
| CSB-PA040150 | Phospho-RAF1 (S642) Antibody | Human,Mouse,Rat | WB,ELISA |
| CSB-PA040151 | Phospho-RAF1 (Y341) Antibody | Human,Mouse,Rat | WB,IHC,ELISA |
| CSB-PA040152 | Phospho-RAF1 (S296) Antibody | Human,Mouse,Rat | WB,IHC,ELISA |
| CSB-PA040153 | Phospho-RAF1 (S621) Antibody | Human,Mouse,Rat,Monkey | WB,IHC,IF,ELISA |
| CSB-PA040154 | Phospho-RAF1 (T269) Antibody | Human,Mouse,Rat | IHC,ELISA |
| CSB-PA040155 | Phospho-RAF1 (S289) Antibody | Human,Mouse,Rat | IHC,ELISA |
| CSB-PA040156 | RAF1 Antibody | Human,Mouse,Rat | WB,ELISA |
| CSB-PA040157 | RAF1 Antibody | Human,Mouse,Rat | WB,IHC,ELISA |
| CSB-PA040158 | RAF1 Antibody | Human,Mouse,Rat | WB,IHC,ELISA |
| CSB-PA040160 | RAF1 Antibody | Human,Mouse,Rat | IHC,ELISA |
| CSB-PA080220 | RAF1 Antibody | Human | WB,IHC |
| CSB-PA010547 | RAF1 Antibody | Human,Mouse,Rat | WB,ELISA |
| CSB-PA694669 | RAF1 Antibody | Human,Mouse,Rat | ELISA,IHC |
| CSB-RA019284A621phHU | Phospho-RAF1 (S621) Antibody | Human | ELISA, WB, IF |
| CSB-RA019284A43phHU | Phospho-RAF1 (S43) Antibody | Human | ELISA, IF |
RAF1 Antibodies for Human
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA019284LA01HU | RAF1 Antibody | Human | ELISA, WB, IHC, IF, IP |
| CSB-PA019284LB01HU | RAF1 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA019284LC01HU | RAF1 Antibody, FITC conjugated | Human | ELISA |
| CSB-PA019284LD01HU | RAF1 Antibody, Biotin conjugated | Human | ELISA |
RAF1 Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP019284HU CSB-BP019284HU CSB-MP019284HU |
Recombinant Human RAF proto-oncogene serine/threonine-protein kinase(RAF1) | Yeast Baculovirus Mammalian cell |
| CSB-EP019284HU | Recombinant Human RAF proto-oncogene serine/threonine-protein kinase(RAF1) | E.coli |
2-hydroxyisobutyryl-HIST1H3A (K23) Antibody
Applications: ELISA, WB, IF, ChIP
Species Reactivity: Human
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