Code | CSB-RA019284A43phHU |
Size | US$210 |
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Application | Recommended Dilution |
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IF | 1:20-1:200 |
The process of generating the phospho-RAF1 (S43) recombinant monoclonal antibody commences with the isolation of genes responsible for coding the RAF1 antibody from rabbits previously immunized with a synthesized peptide derived from the human RAF1 protein phosphorylated at S43. These antibody genes are subsequently introduced into expression vectors, and the genetically modified vectors are carefully transfected into host suspension cells. After successful transfection, positive cells are cultured to promote the robust expression and secretion of antibodies. Following this cultivation phase, the phospho-RAF1 (S43) recombinant monoclonal antibody undergoes a meticulous purification process utilizing affinity chromatography, effectively separating the antibody from the surrounding cell culture supernatant. Ultimately, the functionality of the antibody is comprehensively evaluated through ELISA and IF, unequivocally confirming its capacity to interact with the human RAF1 protein phosphorylated at S43.
Phosphorylation of RAF1 at S43 is a crucial regulatory event in the MAPK signaling pathway, impacting cell growth, survival, and differentiation. Dysregulation of this phosphorylation event can have significant implications in cancer and other diseases driven by aberrant signaling.
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