Recombinant Mouse RAF proto-oncogene serine/threonine-protein kinase (Raf1)

1. that ablation of c-RAF expression in advanced tumors driven by Kras(G12V)/Trp53 mutations leads to significant tumor regression with no detectable appearance of resistance mechanisms PMID: 29395869
2. Our findings indicated that miR-195 inhibited WT and L613V RAF-1 induced hyperactive osteoblast differentiation in MC3T3-E1 cells by targeting RAF-1 PMID: 29197556
3. Data indicate that Raf-1 proto-oncogene, serine-threonine kinase (RAF1) is a negative regulator of hepatocarcinogenesis. PMID: 28000790
4. Results indicate that proto-oncogene c-RAF (CRAF) function affects postmitotic neural cell differentiation and points to a critical role of CRAF-dependent growth factor signaling pathway in the postmitotic development of adult-born neurons. PMID: 29590115
5. our studies demonstrate that PDE8A inhibition by enzymatic inhibitors or PDE8A-Raf-1 kinase complex disruptors decreases Teff cell adhesion and migration under flow, and represents a novel approach to target T cells in inflammation PMID: 28851628
6. Neuroprotective (arylthio)cyclopentenone prostaglandins directly bind to c-Raf protein and protect cells from down-regulation of the c-Raf protein itself, resulting in protection against oxidative stress. PMID: 28888848
7. We confirmed that Raf1(L613V) knock-in confers a NS-like phenotype, including cardiac hypertrophy. Active RSK3 was increased in Raf1(L613V) mice. Constitutive RSK3 gene deletion prevented the Raf1(L613V)-dependent concentric growth in width of the cardiac myocyte and attenuated cardiac hypertrophy in female mice. PMID: 26940993
8. C-Raf involves in osteoblast survival in response to mechanical stress. PMID: 27240957
9. Dual inhibition of c-Raf and soluble epoxide hydrolase by t-CUPM prevents mutant KrasG12D-initiated murine pancreatic carcinoma growth. PMID: 26683769
10. Simultaneous inhibition of sEH and c-RAF prevents chronic pancreatitis and murine pancreatic intraepithelial neoplasia in LSL-KrasG(1)(2)D/Pdx-1-Cre mice. PMID: 26722025
11. under normal physiological conditions, PTEN suppresses AKT activity to maintain activation of the RAF1/ERK signaling pathway, which in turn maintains normal function of the initial segment and therefore, normal sperm maturation. PMID: 25512490
12. Raf-MAPK-dependent psoriatic-like epidermal hyperplasia requires Stat3 signaling in keratinocytes PMID: 23870655
13. Computational modeling revealed that complexes containing H-ras conformers and galectin-1 affect both the number and lifetime of nanoclusters and thus determine the specific Raf effector recruitment. PMID: 24569991
14. This EPOR/CR3 system may serve as a useful model to evaluate the unknown Raf kinase pathway and the effects of signal transduction inhibitors for Raf as a target PMID: 23820731
15. Data indicate that caspase-8 activity is lost upon deletion of c-FLIPL, and p43FLIP rescues caspase-8 activity through Raf1, TRAF2, and RIPK1 association, augmenting ERK and NF-kappaB pathways. PMID: 24275659
16. Endothelial ERK signaling is critical for both arteriogenesis and arterial-venous patterning and that RAF1 Ser(259) phosphorylation plays a critical role in preventing unopposed ERK activation. PMID: 23529931
17. Raf1(D486N/+) (here D486N/+) female mice exhibited a mild growth defect. Male and female D486N/D486N mice developed concentric cardiac hypertrophy and incompletely penetrant, but severe, growth defects. PMID: 22826437
18. Data suggest that K-Ras-responsive tissues exhibit activated Ras/Raf/Erk1/2 signaling, rapidly form premalignant tumors, and activate potent antitumor responses that effectively prevent further malignant progression. PMID: 22532587
19. C-Raf but not B-Raf is required for the proliferative effects of K-Ras(G12D) in primary epithelial cells. PMID: 22043453
20. Arrestin-2 and -3 association with beta(2)-adrenergic receptor (beta2AR) significantly enhanced ERK2 binding, but showed little effect on arrestin interactions with the upstream kinases c-Raf1 and MEK1. PMID: 22174878
21. Raf-1 specifically and acutely regulates insulin 2 mRNA via negative action on Foxo1 PMID: 21817126
22. Raf1, but not BRAF, modulates BCR-ABL-dependent ERK activation and transformation of hematopoietic cells PMID: 21715303
23. Data show that through HINT1, the MOR facilitates the cross-talk of two NO- and zinc-regulated signal-transduction pathways, PKC/Src and Raf-1/ERK1/2, implicated in the negative control of morphine effects. PMID: 21235400
24. c-Raf plays a unique role in mediating K-Ras signaling in non-small cell lung carcinoma and makes it a suitable target for therapeutic intervention. PMID: 21514245
25. enhanced MEK-ERK activity is critical for causing hypertrophic cardiomyopathy and other RAF1-mutant Noonan syndrome phenotype PMID: 21339642
26. Results define a novel mechanism of survivin regulation by EGF through the Raf-1/MEK/ERK pathway in pancreatic beta-cells, via prolongation of survivin protein half-life and inhibition of the ubiquitin-mediated proteasomal degradation pathway. PMID: 20807437
27. Protein kinase C epsilon regulates translocator protein Tspo gene expression through a proto-oncogene protein Raf-1-MAP-kinase signal transduction pathway, acting at least in part through c-Jun and STAT3 transcription factors. PMID: 20469933
28. Activation of the Raf signaling pathway is involved in the epidermal hyperplasia and the neutrophil-dominant cutaneous inflammatory reactions which are characteristics of psoriasis. PMID: 20188517
29. analysis of molecular pertubations allowing dysplasia to progress further to adenocarcinoma induced by exaggerted c-Raf kinase activity PMID: 19812696
30. Rok-alpha inhibition by Raf-1 relies on an intermolecular interaction between the Rok-alpha kinase domain and the cysteine-rich Raf-1 regulatory domain (Raf-1reg). PMID: 19948477
31. Cyclic AMP blocks cell growth through Raf-1-dependent and Raf-1-independent mechanisms. PMID: 11997508
32. Raf regulates glycolysis in mouse cells PMID: 12123723
33. Data indicate that protein kinase C alpha and RAF1 are important in the translational regulation of lipoprotein lipase in adipocytes and that the mechanism of regulation is probably through an ERK-independent pathway. PMID: 12149272
34. The observed alpha-adrenergic effects on lung neutrophil activation appeared to involve primarily the extracellular signal-regulated kinase pathway at the upstream kinase Raf. PMID: 12223526
35. elucidation of essential function in erythropoiesis PMID: 12438425
36. Data suggest that hydrogen peroxide regulates the activity of NF-kappaB by MEK1 activation through a MEKK1-dependent but Ras/Raf-independent mechanism. PMID: 12532330
37. RafS259 mutants genetically separate MEK/ERK activation from the ability of Raf-1 to induce transformation and differentiation. The results further suggest that RafS259 mutants inhibit signaling pathways required to promote these biological processes. PMID: 12612072
38. upon activation of protein kinase A, Raf-1 is phosphorylated and 14-3-3 binds, blocking Raf-1 recruitment to the plasma membrane and preventing its activation PMID: 12801936
39. Raf-1 is dispensable for megakaryocytopoiesis, and for thrombopoietin-induced ERK1/2 activation in primary megakaryocyte-lineage cells. PMID: 14576068
40. After stimulation of G-protein-coupled receptors, RKIP dissociates from its known target, Raf-1, to associate with GRK-2 and block its activity PMID: 14654844
41. Raf-1 kinase activity is essential for cardiac hypertrophy and cardiomyocyte survival in response to pressure overload. PMID: 15289381
42. Ras-independent activation of Raf-1 protein kinase by phorbol myristate acetate and H2O2 is enhanced by src tyrosine kinase inhibitor PP2 PMID: 15356004
43. These results indicate that Raf-1 promotes cardiomyocyte survival through a MEK/ERK-independent mechanism. PMID: 15467832
44. a novel Jun-Dmp1 pathway directly links oncogenic Ras-Raf signaling and p19(Arf), independent of the classical cyclin D1/Cdk4-Rb-E2F pathway PMID: 15601844
45. proteomic analysis of Raf-1 signaling complexes was used to show that Raf-1 counteracts apoptosis by suppressing the activation of mammalian sterile 20-like kinase (MST2) PMID: 15618521
46. identifed six phosphorylation sites that contribute to the downregulation of Raf-1 after mitogen stimulation; phosphorylation of all six sites requires MEK signaling, indicating a negative feedback mechanism PMID: 15664191
47. RAF1, protein kinase A, and protein kinase Cepsilon have roles in regulates amphotropic murine leukemia virus envelope protein-induced syncytium formation PMID: 15741175
48. RAf1 regulates rho signaling and cell migration. PMID: 15753127
49. Pak1-dependent Raf-1 phosphorylation regulates its mitochondrial localization, phosphorylation of BAD, and Bcl-2 association PMID: 15849194
50. Raf-1 and A-Raf have a combined role in controlling physiological transient ERK activation and in maintenance of cell cycle progression at its usual rate. PMID: 15856007

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KEGG: mmu:110157

STRING: 10090.ENSMUSP00000000451

UniGene: Mm.184163