Code | CSB-RA019284A259phHU |
Size | US$210 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
To create the phospho-RAF1 (S259) recombinant monoclonal antibody, the first step involves obtaining the genes responsible for coding this RAF1 antibody. These genes are sourced from rabbits previously exposed to a synthesized peptide derived from the human RAF1 protein phosphorylated at S259. Subsequently, these antibody genes are cloned into specialized expression vectors. Following this genetic modification, the vectors are carefully transfected into host suspension cells, which are then diligently cultivated to promote the expression and secretion of antibodies. The phospho-RAF1 (S259) recombinant monoclonal antibody is then subjected to a meticulous purification process using affinity chromatography, effectively separating the antibody from the surrounding cell culture supernatant. Finally, the antibody's functionality is rigorously assessed through a battery of tests, including ELISA, WB, IHC, and IF, confirming its capability to effectively interact with the human RAF1 protein phosphorylated at S259.
Phosphorylation of RAF1 at S259 is a critical regulatory event in the MAPK signaling pathway, helping to control cell proliferation, differentiation, and survival. Dysregulation of this phosphorylation event can have significant implications for cancer and other diseases driven by abnormal signaling pathways.
There are currently no reviews for this product.