Raf1 Antibody

Code CSB-PA950764
Size US$297
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  • Western blot analysis of extract from mouse brain tissue and C6 cells using Raf-1 Antibody
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Product Details

Full Product Name
Rabbit anti-Rattus norvegicus (Rat) Raf1 Polyclonal antibody
Uniprot No.
Target Names
Raf1
Alternative Names
Raf1 antibody; Raf antibody; RAF proto-oncogene serine/threonine-protein kinase antibody; EC 2.7.11.1 antibody; Proto-oncogene c-RAF antibody; cRaf antibody; Raf-1 antibody
Raised in
Rabbit
Species Reactivity
Rat
Immunogen
Peptide sequence around aa. 641-645(T-S-P-R-L ) derived from Rat Raf-1.
Immunogen Species
Rattus norvegicus (Rat)
Clonality
Polyclonal
Purification Method
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide
Concentration
It differs from different batches. Please contact us to confirm it.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Tested Applications
ELISA,WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:1000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. Phosphorylates TNNT2/cardiac muscle troponin T.
Gene References into Functions
  1. Wild-type PKGII phosphorylated Raf-1 at Ser-43 in a cGMP-dependent manner, but a PKGII D412A/R415A mutant, which has a low affinity for cGMP, did not. PMID: 28057484
  2. Raf-1 kinase contributes to cerebral vasospasm and early brain injury after subarachnoid hemorrhage by enhancing the activation of the Raf-1/ERK1/2 and Raf-1/NF-kappaB signaling pathways. PMID: 25367879
  3. sorafenib exhibits a regulatory role on the occurrence of LVH following AB in rats by blocking the rise in growth factors PDGF-BB and TGFbeta1, activation of the corresponding c-Raf-ERK1/2 signaling pathway PMID: 24885948
  4. Our results demonstrate that XIAP plays a new role as a negative regulator of neurotrophin-induced neurite outgrowth and neuronal differentiation in developing neurons. PMID: 23928917
  5. Induction of streptozotocin diabetes resulted in decreased level of GLP-1R and increased levels of IRS-1 and Raf-1 in the liver. PMID: 22983684
  6. Data show that a dimeric Raf kinase inhibitor protein (RKIP) mutant displayed a higher affinity to G protein-coupled receptor kinase (GRK) 2, but a lower affinity to Raf1. PMID: 22610096
  7. Protein arginine methyltransferase 5 regulates ERK1/2 signal transduction amplitude and cell fate through CRAF. PMID: 21917714
  8. RAF1 mutants caused hypertrophy signaled via nuclear factor of activated T cells PMID: 21440552
  9. These results indicate the initial robust activation of ERK phosphorylates Raf-1 at Ser289/296/301, resulting in Raf-1inhibition and then prompt inactivation of ERK following sublethal preconditioning ischemia. PMID: 20953701
  10. Raf-1 played a significant role in sustained morphine-mediated paradoxical pain sensitization and antinociceptive tolerance in vivo. PMID: 20718739
  11. Raf-1 kinase plays an important role in the regulation of vascular contractility through regulation of calcium sensitization. PMID: 20110729
  12. the role of Raf-1 in v-Ha-ras transformation PMID: 11933072
  13. Affinity with Raf is sufficient for Ras to efficiently induce rat mammary carcinomas PMID: 12538354
  14. Raf-1 has a role in modulating integrin-stimulated ERK activation when bound to CAMKII PMID: 12954639
  15. Changes in flexibility upon protein-protein complex formation of H-Ras & the Ras-binding domain of C-Raf1 have been investigated using the molecular framework approach FIRST and molecular dynamics simulations of in total approximately 35 ns length. PMID: 15211515
  16. selective modulation of c-Raf-1 but not B-Raf activation by RKIP can limit the dynamic range of the MAPK signaling response to growth factors and may play a critical role in growth and development PMID: 15886202
  17. aldosterone seems to exert mainly MR-induced effects that stimulate c-Raf, MEK1/2, MAPK1/2, the activities of CDK2 and CDK4, and the cell-cycle progression in mesangial cells PMID: 15975997
  18. c-raf.1 expression was located in the late basophilic lesions associated with hepatocellular carcinoma. PMID: 17554210
  19. Our results suggest that ROS production mediates the biphasic activation of ERK through different signaling cascades following post-ischemic reperfusion. Mediation of these signaling pathways involves changes in Raf-1 phosphorylation at different sites. PMID: 18243549
  20. AhR is a master regulator of c-raf and propose cross-talk between AhR and the mitogen-activated protein kinase signaling pathway. PMID: 18708364
  21. These studies identify Raf-dependent cTnT-Thr(206) phosphorylation as a novel mechanism that would link growth factor-dependent signaling pathways to dynamic changes in cardiac contractile function. PMID: 19381846

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Subcellular Location
Cytoplasm. Cell membrane. Mitochondrion. Nucleus.
Protein Families
Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily
Database Links
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