RAF1 (Ab-259) Antibody

Datasheet
Code CSB-PA242762
Product Type Polyclonal Antibody
Size US$297
Uniprot No. P04049
Image
  • Western blot analysis of extracts from Hela, K562 and HT29 cells using Raf1(Ab-259) Antibody.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1(Ab-259) Antibody.
Immunogen Peptide sequence around aa.257~261 (S-T-S-T-P) derived from Human RAF.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous level of total Raf1 protein.
Tested Applications ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:200
Relevance Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.

Dougherty M K, et al. (2005) Mol Cell. 17(2): 215-224.
Hekman M, et al. (2005) FEBS Lett. 579(2): 464-468.
Avruch J,et al. (1994) Trends Biochem. Sci. 19, 279-283.
Chong H, et al. (2001) EMBO J. 20, 3716-3727.
King A J, et al. (1998) Nature. 396:180-183.
Fabian J R, et al. (1993) Mol Cell Biol. 13: 7170-7179.
Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality Polyclonal
Alias C-RAF; C-Raf; CRAF
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Target Names RAF1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases
Involvement in disease Noonan syndrome 5 (NS5); LEOPARD syndrome 2 (LPRD2); Cardiomyopathy, dilated 1NN (CMD1NN)
Subcellular Location Cytoplasm, Cell membrane, Mitochondrion, Nucleus
Protein Families Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily
Tissue Specificity In skeletal muscle, isoform 1 is more abundant than isoform 2.
Database Links

HGNC: 9829

OMIM: 164760

KEGG: hsa:5894

STRING: 9606.ENSP00000251849

UniGene: Hs.159130

Pathway cAMP signaling pathway
Chemokine signaling pathway
ErbB signaling pathway
Estrogen signaling pathway
Jak-STAT signaling pathway
MAPK signaling pathway
Melanogenesis
mTOR signaling pathway
PI3K-Akt signaling pathway
VEGF signaling pathway
Focal adhesion
Gap junction
Regulation of actin cytoskeleton
Vascular smooth muscle contraction
Apoptosis
Autophagy
Cellular senescence
MAPK Erk pathway
Ras signaling pathway
Signaling pathways regulating pluripotency of stem cells
B cell receptor signaling pathway
Fc epsilon RI signaling pathway
Fc gamma R-mediated phagocytosis
Natural killer cell mediated cytotoxicity
T cell receptor signaling pathway
Apelin signaling pathway
cGMP-PKG signaling pathway
FoxO signaling pathway
Insulin signaling pathway
Oxidative Phosphorylation
Phospholipase D signaling pathway
Rap1 signaling pathway
Sphingolipid signaling pathway
Excitatory synapse pathway
Neurotrophin signaling pathway

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