Tumor necrosis factor receptor superfamily member 6 is a protein in humans that is encoded by FAS gene. Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
The following fas reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
fas Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA06354A0Rb | FAS Antibody |
Human | ELISA, IHC, IF |
CSB-PA146193 | FAS Antibody |
Human | ELISA,WB |
CSB-PA942791 | FAS Antibody |
Human | ELISA,WB,IHC |
CSB-PA001488 | FAS Antibody |
Human | WB, IHC, ELISA |
CSB-PA002481 | FAS Antibody |
Human | WB, IF, ELISA |
CSB-PA006041 | FAS Antibody |
Human | WB, ELISA |
CSB-PA009527 | Phospho-FAS (Y291) Antibody |
Human | IF, ELISA |
CSB-PA185779 | FAS Antibody |
Human | ELISA,WB,IHC |
CSB-RA252392A0HU | FAS Recombinant Monoclonal Antibody |
Human | ELISA, WB, IHC |
fas Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP008433HU CSB-EP008433HU CSB-BP008433HU CSB-MP008433HU CSB-EP008433HU-B |
Recombinant Human Tumor necrosis factor receptor superfamily member 6 (FAS), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-AP002311HU | Recombinant Human Tumor necrosis factor receptor superfamily member 6 protein (FAS), partial (Active) |
E.coli |
CSB-CF008433HU(A4) | Recombinant Human Tumor necrosis factor receptor superfamily member 6 (FAS) |
in vitro E.coli expression system |
CSB-MP008433HU(A4) | Recombinant Human Tumor necrosis factor receptor superfamily member 6 (FAS)-VLPs |
Mammalian cell |
fas Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP737175RA CSB-EP737175RA CSB-BP737175RA CSB-MP737175RA CSB-EP737175RA-B |
Recombinant Rat Tumor necrosis factor receptor superfamily member 6 (Fas), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
fas Proteins for Macaca nemestrina (Pig-tailed macaque)
Code | Product Name | Source |
---|---|---|
CSB-YP857720MOX CSB-EP857720MOX CSB-BP857720MOX CSB-MP857720MOX CSB-EP857720MOX-B |
Recombinant Macaca nemestrina Tumor necrosis factor receptor superfamily member 6 (FAS), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
fas Proteins for Macaca mulatta (Rhesus macaque)
Code | Product Name | Source |
---|---|---|
CSB-YP859783MOW CSB-EP859783MOW CSB-BP859783MOW CSB-MP859783MOW CSB-EP859783MOW-B |
Recombinant Macaca mulatta Tumor necrosis factor receptor superfamily member 6 (FAS), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
fas Proteins for Cercocebus atys (Sooty mangabey) (Cercocebus torquatus atys)
Code | Product Name | Source |
---|---|---|
CSB-YP861037DRI CSB-EP861037DRI CSB-BP861037DRI CSB-MP861037DRI CSB-EP861037DRI-B |
Recombinant Cercocebus atys Tumor necrosis factor receptor superfamily member 6 (FAS), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
fas Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Code | Product Name | Source |
---|---|---|
CSB-YP890581MOV CSB-EP890581MOV CSB-BP890581MOV CSB-MP890581MOV CSB-EP890581MOV-B |
Recombinant Macaca fascicularis Tumor necrosis factor receptor superfamily member 6 (FAS), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
fas Proteins for Bos taurus (Bovine)
Code | Product Name | Source |
---|---|---|
CSB-CF008433BO | Recombinant Bovine Tumor necrosis factor receptor superfamily member 6 (FAS) |
in vitro E.coli expression system |
CSB-YP008433BO1 CSB-EP008433BO1 CSB-BP008433BO1 CSB-MP008433BO1 CSB-EP008433BO1-B |
Recombinant Bovine Tumor necrosis factor receptor superfamily member 6 (FAS), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
fas Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-CF008433MO | Recombinant Mouse Tumor necrosis factor receptor superfamily member 6 (Fas) |
in vitro E.coli expression system |
CSB-YP008433MO1 CSB-EP008433MO1 CSB-BP008433MO1 CSB-MP008433MO1 CSB-EP008433MO1-B |
Recombinant Mouse Tumor necrosis factor receptor superfamily member 6 (Fas), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
fas Proteins for Sus scrofa (Pig)
Code | Product Name | Source |
---|---|---|
CSB-CF008433PI | Recombinant Pig Tumor necrosis factor receptor superfamily member 6 (FAS) |
in vitro E.coli expression system |
CSB-YP008433PI1 CSB-EP008433PI1 CSB-BP008433PI1 CSB-MP008433PI1 CSB-EP008433PI1-B |
Recombinant Pig Tumor necrosis factor receptor superfamily member 6 (FAS), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
fas ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E04542h | Human Factor-related Apoptosis,FAS ELISA Kit |
serum, plasma, tissue homogenates | 7.8 pg/mL |
fas ELISA Kit for Rattus norvegicus (Rat)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E07324r | Rat Factor-related Apoptosis,FAS ELISA Kit |
serum, plasma, tissue homogenates | 0.195 ng/mL |
fas ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E04543m | Mouse Factor-related Apoptosis,FAS ELISA Kit |
serum, plasma, cell culture supernates, tissue homogenates | 0.156 ng/mL |
FAS, also known as CD95/Apo-1/TNFRSF6, is a type I transmembrane (TM) protein on the cell surface, and a death receptor that belongs to the TNF-receptor superfamily [1]. Fas contains an N-terminal ligand-binding extracellular domain (ECD), a TM domain, and a C-terminal cytoplasmic death domain (DD) [1]. Expressed at the cell surface as a homotrimer, the FAS receptor implements both apoptotic and non-apoptotic signaling pathways. The ligation of FAS with FAS ligand (FASL) leads to the activation of a caspase cascade that initiates apoptosis [2][3][4]. FAS triggers apoptosis through FADD-mediated recruitment and activation of caspase-8 [5]. Fas–FasL-induced apoptosis is an important mechanism for the maintenance of immune homeostasis &peripheral tolerance, and the surveillance of tumor [6]. Its non-apoptotic cues seem to promote oncogenesis [7]. FAS/CD95 engagement also induces non-apoptotic signaling pathways promoting cell motility, invasiveness [8], inflammation, and organ regeneration. A large fraction of human ALPS (autoimmune lymphoproliferative syndrome) patients have heterozygous inherited mutations in the FAS gene [9]. ALPS is an inherited disorder in which the body cannot properly regulate the number of immune system cells (lymphocytes). In consideration of the multiple roles of Fas–FasL in immune responses and diseases, therapeutic targets of the Fas/FasL pathway might not protect against a specific disease rather than influencing the patient's prognosis.
[1] Wu H, Hymowitz SG. Structure and function of tumor necrosis factor (TNF) at the cell surface. In: Bradshaw RA, Dennis EA, editors. Handbook of cell signaling [J]. Oxford: Academic Press; 2009. pp. 265–275.
[2] Nagata S, Golstein P. The Fas death factor [J]. Science (1995) 267(5203):1449–56.
[3] Chinnaiyan AM, O’Rourke K, et al. FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis [J]. Cell (1995) 81:505–12.
[4] Kischkel FC, Hellbardt S, et al. Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing signaling complex (DISC) with the receptor [J]. EMBO J (1995) 14:5579–88.
[5] Nagata S. Apoptosis by death factor [J]. Cell (1997) 88(3):355–65.
[6] Strasser A, Jost PJ, et al. The many roles of FAS receptor signaling in the immune system [J]. Immunity. 2009 Feb 20;30(2):180-92.
[7] Le Gallo M, Poissonnier A, et al. CD95/Fas, Non-Apoptotic Signaling Pathways, and Kinases [J]. Front Immunol. 2017 Sep 27; 8:1216.
[8] Nijkamp MW, Hoogwater FJ, et al. CD95 is a key mediator of invasion and accelerated outgrowth of mouse colorectal liver metastases following radiofrequency ablation [J]. J Hepatol. 2010 Dec;53(6):1069-77.
[9] Dowdell KC, Niemela JE, et al. Somatic FAS mutations are common in patients with genetically undefined autoimmune lymphoproliferative syndrome [J]. Blood. 2010 Jun 24;115(25):5164-9.