FAS Antibody

Datasheet
Code CSB-PA942791
Product Type Polyclonal Antibody
Size US$297
Uniprot No. P25445
Image
  • Western blot analysis of extracts from LOVO cells, using Fas antibody.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Fas antibody.
Immunogen Synthesized peptide derived from C-terminal of Human Fas.
Raised in Rabbit
Species Reactivity Human
Specificity The antibody detects endogenous levels of total Fas protein.
Tested Applications ELISA,WB,IHC;WB:1:500-1:3000,IHC:1:50-1:100
Relevance Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

Michael J. Pinkoski, Am JPhysiol Gastrointest Liver Physiol, Mar 2000; 278: 354.
Morgan Jenkins, J. Immunol., Aug 1999; 163: 1195.
Nicholas Mitsiades, J Natl Cancer Inst, Oct 1999; 91: 1678 - 1684.
JOS? E. CARDIER, FASEB J, Nov 1999; 13: 1950.

Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality Polyclonal
Alias Apo-1; CD95; FasL receptor; TNFRSF6; TNR6
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Target Names FAS
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Involvement in disease Autoimmune lymphoproliferative syndrome 1A (ALPS1A)
Subcellular Location Isoform 1: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 2: Secreted, SUBCELLULAR LOCATION: Isoform 3: Secreted, SUBCELLULAR LOCATION: Isoform 4: Secreted, SUBCELLULAR LOCATION: Isoform 5: Secreted, SUBCELLULAR LOCATION: Isoform 6: Secreted
Tissue Specificity Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Database Links

HGNC: 11920

OMIM: 134637

KEGG: hsa:355

STRING: 9606.ENSP00000347979

UniGene: Hs.244139

Pathway MAPK signaling pathway
p53 signaling pathway
TNF signaling pathway
Apoptosis
Necroptosis
Natural killer cell mediated cytotoxicity

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