Rat Factor-related Apoptosis,FAS ELISA Kit

Instructions
Code CSB-E07324r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Fas (TNF receptor superfamily, member 6)
Alternative Names Fas ELISA Kit; Apt1 ELISA Kit; Tnfrsf6 ELISA Kit; Tumor necrosis factor receptor superfamily member 6 ELISA Kit; Apo-1 antigen ELISA Kit; Apoptosis-mediating surface antigen FAS ELISA Kit; FASLG receptor ELISA Kit; CD antigen CD95 ELISA Kit
Abbreviation FAS
Uniprot No. Q63199
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat FAS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %95
Range %86-105
1:2Average %96
Range %91-100
1:4Average %96
Range %87-105
1:8Average %96
Range %90-102
Recovery
The recovery of rat FAS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9689-105
EDTA plasma (n=4)9593-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.504 2.532 2.518 2.402
252.312 2.345 2.329 2.213
12.51.884 1.879 1.882 1.766
6.251.111 1.133 1.122 1.006
3.120.573 0.586 0.580 0.464
1.560.363 0.374 0.369 0.253
0.780.241 0.255 0.248 0.132
00.115 0.117 0.116  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity).
Gene References into Functions
  1. Report dysregulation of the Fas/FasL system occurs in an experimental animal model of HELLP syndrome. PMID: 28501275
  2. Vitamin D could alleviate pathological changes, reduce Fas/FasL expression, and attenuate myocardial cell apoptosis in diabetic cardiomyopathy rats, which might be used as a potential effective therapy for the disease. PMID: 28595623
  3. Study found that propofol anesthesia activates Fas/FasL-mediated extrinsic and Bcl-2-dependent intrinsic apoptotic pathways, resulting in caspase-8 and caspase-9 activations, respectively, and established upregulation of caspase-1 and IL-1beta cytokine transcriptions as well as subsequent activation of microglia. Behavioral data revealed that hyperactivity persists through adolescence and young adulthood. PMID: 27189477
  4. NaIO3 activates Fas-mediated cell death, both in the RPE and photoreceptor, and that a small peptide antagonist of the Fas receptor, Met12, significantly reduces the extent of this cell death. PMID: 28346613
  5. Western blotting demonstrated that expression of CD95, FasL and FADD was very weak during embryonic development, but rapidly increased at P14. PMID: 27561622
  6. miR-133b-5p might play a particularly important role in the cardioprotective effect of morphine preconditioning by regulating the target gene Fas PMID: 27919821
  7. Organic pollutants in drinking water impair liver function, with the involvement of Fas and mitochondria-mediated apoptosis in rats. PMID: 26316710
  8. Mip1 over-expression inhibited H2O2-induced apoptosis and upregulation of Fas induced by H2O2 in H9c2 myogenic cells. PMID: 25310648
  9. Chronic methamphetamine exposure will activate cardiac Fas-dependent and mitochondria-dependent apoptotic pathways. PMID: 24307234
  10. Fas protein was up-regulated in rat mesangial cells, and its neutralizing antibody ZB4 partly inhibited M2 macrophage-induced apoptosis PMID: 23973665
  11. The expression of Fas in spermatogenic cells and the sperm aberration of each fluorotic group were higher than control group. PMID: 20568470
  12. Autophagy activation occurs in photoreceptors after retina-retinal pigment epithelium separation. This appears to be, at least in part, dependent on Fas receptor activation and plays role in regulating level of photoreceptor apoptosis. PMID: 21421874
  13. The treatment with cefoperazone sodium and levofloxacin lactate could inhibit the expression of Fas and Bax and enhance the expression of Bcl-2 in rats infected with Vibrio vulnificus. PMID: 19399403
  14. Inhibition of Fas can diminish LPS-induced apoptosis and inflammatory cytokine production in type II alveolar epithelial cell. PMID: 19823951
  15. increased expression on conjunctival epithelial cells of patients with cystic fibrosis PMID: 19642903
  16. Aluminium could influence the activities of learning and memory and could reduce the expression of Bcl-2 and promote the expression of Fas. PMID: 19267062
  17. Results indicate that cytokine-treated beta-cells may possess two pools of Fas protein, one of which is inducible by cytokines and accounts for surface Fas expression, and one which is constitutively expressed in cytoplasmic compartments. PMID: 12683940
  18. critical tyrosine residues of CD95 are targets of the anti-apoptotic action of nitric oxide PMID: 14679192
  19. CD95 regulation involves complex phosphorylations with CD95-tyrosine phosphorylation favoring CD95 membrane trafficking and DISC formation, whereas CD95 Ser/Thr phosphorylation inhibits these processes. PMID: 14699504
  20. epidermal growth factor receptor and CD95 activation are triggered by Src family kinase Yes PMID: 15039424
  21. FAS-mediated apoptosis pathway becomes activated and transcriptionally upregulated after retinal detachment. The peak of FAS activation precedes that of the intrinsic pathway, and inhibition of FAS activation can decrease caspase-9 activity. PMID: 15557468
  22. Adult spinal motor neuron apoptosis is mediated by upstream NO and ONOO- genotoxicity and downstream p53 and Fas activation and is shifted to necrosis by mutant SOD1. PMID: 16000635
  23. In rat islets, constitutive expression of Fas ligand and glucose-induced Fas receptor expression were observed only in 7- to 8-month-old but not in 2- to 3-month-old islets PMID: 16936193
  24. Activation of glial cells and upregulation of Fas suggest that glial cells may contribute to Fas-mediated cell death in the neurodegeneration process of chronic ocular hypertensive retinal insult. PMID: 17045251
  25. Data strongly suggests that transcriptional up regulation of Fas could predispose a group of spermatocytes to Fas ligand triggering apoptosis by the extrinsic and intrinsic pathway. PMID: 17195944
  26. These results suggest that Hsp70 is capable of protecting the cardiomyocyte from stress-induced injury by inhibiting Fas-mediated apoptosis. PMID: 17441510
  27. Apoptosis in our model occurs via both extrinsic and intrinsic pathways, and activation of Fas may play a role. PMID: 18289516
  28. CDK inhibitors suppress MDM2 levels and enhance p53 expression that facilitates bile acid-induced, ceramide-dependent CD95 activation to induce both apoptosis and autophagy in primary hepatocytes PMID: 18614532
  29. Using single-particle EM, we have identified a near continuum of conformations consistent with a remarkable flexibility of FAS. PMID: 19151726

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Subcellular Location Membrane, Single-pass type I membrane protein
Database Links

KEGG: rno:246097

STRING: 10116.ENSRNOP00000025928

UniGene: Rn.162521

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