Recombinant Human Growth-regulated alpha protein(CXCL1) (Active)

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Code CSB-AP000631HU
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Product Details

Purity >97% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 10-50 ng/ml.
Target Names CXCL1
Uniprot No. P09341
Research Area Immunology
Alternative Names C-X-C motif chemokine 1; Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity; alpha); chemokine (C-X-C motif) ligand 1; CINC-1; CXCL1; Cytokine-induced neutrophil chemoattractant 1; Fibroblast secretory protein ; Fsp; Gro 1; Gro A; Gro; GRO protein; alpha; GRO-alpha(1-73); GRO-alpha(6-73); Gro1; GRO1 oncogene (melanoma growth stimulating activity; alpha); GRO1 oncogene (melanoma growth-stimulating activity) ; Gro1 oncogene; GROa; GROA_HUMAN; Growth-regulated alpha protein; KC; KC chemokine; mouse; homolog of; melanoma growth stimulatory activity alpha ; Melanoma growth stimulatory activity; Melanoma growth stimulatory activity; alpha; MGSA alpha ; MGSA; MGSA-a; N51; NAP-3; NAP3; Neutrophil-activating protein 3; Platelet-derived growth factor-inducible protein KC; Scyb 1; Scyb1; Secretory protein N51; Small inducible cytokine subfamily B; member 1
Species Homo sapiens (Human)
Source E.coli
Expression Region 35-107aa
Complete Sequence ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN
Mol. Weight 7.9 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
Gene References into Functions
  1. The chemokine Gro1 induced in response to inflammation triggers senescence and arrests development of new neurons in the hippocampus and that the magnitude of this response is sex-dependent. PMID: 30201019
  2. CXCL1 displays a specific microRNA (miR) upregulated by the prototypical colon cancer onco-miR miR-105. PMID: 30115896
  3. These results show that AKIP1 is crucial in cervical cancer angiogenesis and growth by elevating the levels of the NF-kappaB-dependent chemokines CXCL1, CXCL2, and CXCL8. PMID: 29520695
  4. Adipose stromal cells recruitment to tumours, driven by CXCL1 and CXCL8, promotes prostate cancer progression. PMID: 27241286
  5. CXCL1, which is produced by breast cancer cells, can promote cancer growth and development PMID: 29438938
  6. miR-204 inhibits cell proliferation in gastric cancer by targeting CKS1B, CXCL1 and GPRC5A. PMID: 29283424
  7. Thrombocytosis was more prevalent in patients with inflammatory breast cancer (IBC)than in those with non-IBC and it was associated with poor prognosis. GRO and TGF-beta were associated with thrombocytosis in IBC PMID: 28831670
  8. the plasma concentrations of CXCL1 indicated the disease activity and prognosis in interstitial pneumonia with autoimmune features (IPAF). Thus, the CXCL1/CXCR2 axis appears to be involved in the progression of IPAF. PMID: 27958346
  9. CXCL1/8 secreted by adipose-derived mesenchymal stem cells could promote breast cancer angiogenesis. PMID: 28514506
  10. GROA overexpression is associated with invasion in triple negative breast cancer. PMID: 28560447
  11. The results of the transwell chemotaxis assay also supported the above results. Our data suggest that APN can promote h-JBMMSC chemotaxis by up-regulating CXCL1 and CXCL8 PMID: 28176455
  12. This study highlighted CAF-secreted CXCL1 as an attractive target to reverse tumor radioresistance. PMID: 28518141
  13. we identified the microRNA miR-200a as a putative post-transcriptional regulator of CXCL1 in hepatocellular carcinoma PMID: 27542259
  14. study demonstrates that CXCL1 can transform NOFs into senescent CAFs via an autocrine mechanism PMID: 29360827
  15. Association of polymorphic markers of chemokine genes, their receptors, and CD14 gene with coronary atherosclerosis PMID: 29369549
  16. GROalpha high in the tumor microenvironment can be used as potential indicators for the progression of non-small cell lung cancer PMID: 28375674
  17. IL-8, but not the CXCL1 circuit, is critical for the regulation of thyroid cancer stem cells. PMID: 27577959
  18. this study shows that CXCL1 is expressed in epithelium of the endometrium with adenomyosis and demonstrate that VEGF is capable of inducing CXCL1 expression PMID: 27665197
  19. Taken together, the present study indicates that IL-33 localized in the human atherosclerotic plaque increases GRO-alpha mRNA expression and protein secretion via activation of ERK1/2, JNK, and NF-kappaB in HUVECs, suggesting that IL-33 plays an important role in the pathophysiology and development of atherosclerosis. PMID: 28637660
  20. Study provides the first evidence that primary malignant cell-secreted VEGFA stimulates tumor-associated macrophages to produce CXCL1, which recruits CXCR2-positive MDSCs to form a premetastatic niche to promote liver metastases. PMID: 28455419
  21. the presence of elevated circulating levels of VEGF and CXCL1 are predictive of liver and lung metastasis, respectively of colorectal cancer. PMID: 28870907
  22. This study describe elevated levels of CXCL1 and it receptor in the Solid Component and Cyst Fluid of Human Adamantinomatous Craniopharyngioma, relative to other pediatric brain tumors and normal cerebral tissue. PMID: 28859336
  23. The expressions of CXCL1 in cancer cells and CXCR2 in stromal cells are useful prognostic factors for gastric cancer patients PMID: 28575019
  24. CXCL1 secreted by tumor-associated lymphatic endothelial cells promotes lymph node metastasis of gastric cancer through integrin beta1/FAK/AKT signaling pathway. PMID: 27832972
  25. S100A9 and S100A12 may have a role in the pathogenesis of pneumonia: S100A9 and CXCL1 may contribute solely in mild pneumonia, and CCL5 and CXCL11 may contribute in severe pneumonia. PMID: 28381820
  26. These findings support a role for CXCL1 and IL-8 in cystic fibrosis lung disease severity and identify STAT3 as a modulating pathway. PMID: 27799352
  27. Results suggest that CXCL1 is a key molecular link between senescence of stromal fibroblasts and tumor growth. PMID: 27092462
  28. Increased IL-8 and CXCL1 transcription in T84 and THP-1 cells compared to that in wild-type EPEC. PMID: 27297392
  29. CXCL1 signaling in the tumor microenvironment is highly responsible for repeated intravesical recurrence, disease progression, and drug resistance through enhanced invasion ability. In conclusion, disrupting CXCL1 signaling to dysregulate this chemokine is a promising therapeutic approach for human UCB. PMID: 27690238
  30. Silencing of the CXCL1 gene inhibits HGC803 cell migration and invasion. The positive expression of CXCL1 is correlated with poor survival of gastric cancer patients and CXCL1 is an independent prognostic factor for gastric cancer. PMID: 27748927
  31. These results demonstrate that tumor-derived CXCL1 contributes to TANs infiltration in lung cancer which promotes tumor growth. PMID: 27446967
  32. the serum levels of the soluble factors sCD40L and CXCL1 are not associated with endometriosis and are not suitable as biomarkers for disease diagnosis. PMID: 27190986
  33. Elevated expression of GRO-alpha in cytoplasm of cancer cells (hazard ratio [HR] = 5.730, P = 0.007) and stroma (HR = 3.120, P = 0.022) were independent prognostic factors of pancreatic cancer. T classification (HR = 2.130, P = 0.023), lymphatic metastasis (HR = 4.211, P = 0.009) and TNM classification (HR = 0.481, P = 0.031) were also prognostic predictors in PC patients. PMID: 27472713
  34. The novel findings reveal the critical role of NLRP12-IL-17A-CXCL1 axis in host defense by modulating neutrophil recruitment against Klebsiella pneumoniae. PMID: 26349659
  35. increased amounts released by neutrophils from fibromyalgia patients PMID: 26341115
  36. these findings suggest that CXCL1 plays critical roles in the growth and apoptosis of hepatocellular carcinoma PMID: 26499374
  37. BBP also stimulated the production of CXCL1/GROalpha by TADCs, which increased the angiogenesis of breast cancer in a mouse model PMID: 26397389
  38. Intense glomerular CXCL1 expression was observed in biopsy specimens from patients with lupus nephritis PMID: 25471749
  39. hCXCL1-GAG interactions provide stringent control over regulating chemokine levels and receptor accessibility and activation, and that chemotactic gradients mediate cellular trafficking to the target site. PMID: 26721883
  40. Urine CXCL1 is a promising, non-invasive molecular marker for tumor detection and outcome prediction in patients with bladder cancer . PMID: 26406865
  41. CXCR2-CXCL1 axis is correlated with neutrophil infiltration and predicts a poor prognosis in hepatocellular carcinoma PMID: 26503598
  42. Polymorphisms in the promoter regions of the CXCL1 and CXCL2 genes contribute to increased risk of alopecia areata in the Korean population PMID: 26345899
  43. VEGF markedly induces CXCL1 release in A549 lung epithelial cells. PMID: 23665907
  44. TGF-beta negatively regulates CXCL1 expression in CAFs through Smad2/3 binding to the promoter, and through suppression of HGF/c-Met autocrine signaling PMID: 26252654
  45. work shows parallel networks of necroptosis-induced CXCL1 and Mincle signalling that promote macrophage-induced adaptive immune suppression and thereby enable pancreatic ductal adenocarcinoma progression PMID: 27049944
  46. High CXCL1 expression is a poor prognostic biomarker in metastatic colorectal cancer. PMID: 26104296
  47. urinary CXCL1 as a new non-invasive predictor of IgAN progression PMID: 25816025
  48. CXCL1 expression was a negative prognostic factor. PMID: 25175281
  49. There was a significant positive correlation between CXCL-1 levels in the vitreous and the extent of the retinal detachment. PMID: 25766782
  50. CXCL1 expression was highly upregulated in patients with alcoholic hepatitis. PMID: 25930080
  51. miR141-CXCL1-CXCR2 signaling-induced Treg recruitment regulates metastases and survival of non-small cell lung cancer. PMID: 25349304
  52. CXCL1 and CXCL2 chemokines stimulate osteoclast differentiation in vitro PMID: 25802102
  53. The released CXCL1 enhanced monocyte migration. PMID: 25301363
  54. Elevated CXCL1 expression in breast cancer stroma predicts poor prognosis and is inversely associated with expression of TGF-beta signaling proteins. PMID: 25344051
  55. SF of BC OA displayed significantly higher concentrations for a number of proinflammatory cytokines [CXCL1, eotaxin, interferon (IFN)-gamma, interleukin (IL)-7, IL-8, IL-9, IL-12]. PMID: 25393692
  56. TNF-alpha stimulates CXCL1 release from human endothelial cells through JNK-mediated CXCL1 mRNA expression and p38 MAPK- and PI-3K-mediated CXCL1 secretory processes. PMID: 24487964
  57. CXCL1 increases local tumor growth through activation of VEGF signaling. PMID: 25641338
  58. In CSF, CXCL1 is up-regulated in opioid-tolerant patients (and rodents). PMID: 25383568
  59. High CXCL1 stromal protein expression is highly specific for the tumorous transition in cases of sporadic colorectal neoplasias. PMID: 24999605
  60. The silencing of the pro-angiogenic chemokine GRO-alpha is proportionally correlated with decreased expression of CXCR2 in pro-inflammatory cytokine-stimulated SGEC indicating the GRO-alpha/CXCR2 complex as a novel therapeutic target for Sjogren's syndrome. PMID: 23052840
  61. CXCL1 mRNA expression was significantly higher in the lymphocytes of Thromboangiitis obliterans patients than control subjects. PMID: 23567329
  62. The present study showed that nasal fibroblasts can produce GRO-alpha and IL-8, and their production is remarkably enhanced by IL-17 stimulation, thereby clarifying the mechanism of the IL-17 mediated neutrophil infiltration in nasal polyps. PMID: 25135733
  63. Four genes showed increased expression in the epithelium of OLP patients: CD14, CXCL1, IL8, and TLR1, and at least two of these proteins, TLR1 and CXCL1, were expressed at substantial levels in oral keratinocytes. PMID: 24581860
  64. CXCL1 is a negative regulator of mast cell chemotaxis to airway smooth muscle cell products in vitro. PMID: 24588865
  65. This protein displays antimicrobial activity against E. coli and S. aureus. PMID: 12949249
  66. CXCL1 inhibits airway smooth muscle cell migration via activation of the ERK-1/2 MAP kinase pathway. PMID: 24981451
  67. Normal keratinocytes and cancer cells can stimulate the CXCL-1 secretion from normal dermal fibroblasts and stromal fibroblasts from squamous cell carcinoma. PMID: 23890481
  68. decreased serum levels in patients with alopecia areata PMID: 23502337
  69. The rs4074 single nucleotide polymorphism within the CXCL1 gene is associated with increased CXCL1 blood levels and increased risk for development of cirrhosis in alcoholics. PMID: 24260493
  70. Increased CXCL1/CXCL8 instigated polymorphonuclear leukocyte adhesion to cerebral microvascular endothelial cells, possibly contributing to Diabetic ketoacidosis-associated intracranial vascular complications. PMID: 24619879
  71. Expression of the CXCL8, CXCL6 and CXCL1 genes are under the primary control of 1,25-dihydroxyvitamin D3 and its receptor. PMID: 24250750
  72. CXCL1 is a target for the embryos' secretion product IL-1beta in decidualised endometrial stromal cells during the peri-implantation period. PMID: 23953856
  73. IgA1 complexes from IgA nephropathy patients could up-regulate the secretion of CXCL1 and TGF-beta1 in mesangial cells. PMID: 24023680
  74. findings of this study demonstrated that serum concentrations of CXCL1 and CXCL12 were elevated in SCD patients when compared with controls PMID: 24600982
  75. CXCL1 expressed by monocytes and CXCR2 on HBMEC is involved in monocytes migrating from blood to brain in AD patients PMID: 23967336
  76. The CXCL1 gene and particularly T allele of rs3117604 was associated with ischemic stroke. PMID: 23198952
  77. findings of the current study suggest a broad involvement of the chemokine GRO-alpha/CXCR2 system in Sjogren's syndrome pathogenesis that is mediated by the involvement of ADAM17 PMID: 23389772
  78. A novel miRNA, miR-7641, significantly suppressed CXCL1 expression in embryonic stem cells. PMID: 23444042
  79. IL-1alpha regulated its sub-network genes including CXCL1, CXCL10, and ICAM1 mRNA levels in time dependent but not in concentration dependent manner PMID: 24273934
  80. this is the largest study describing increased CXCL1 protein expression in more aggressive phenotypes in human bladder cancer PMID: 23815949
  81. Neutrophil priming led to the rapid expression of a common set of transcripts for cytokines, chemokines and cell surface receptors (CXCL1, CXCL2, IL1A, IL1B, IL1RA, ICAM1). PMID: 23554905
  82. expressed and secreted from endothelial cells PMID: 23732813
  83. When expressed, CXCL1 is limited to small areas with faint staining and PCa progression does not rely on CXCL1 expression. PMID: 23334998
  84. sequence-linked, post-transcriptional activities provide substantial mechanistic diversity in the control of GRO family chemokine gene expression. PMID: 23519936
  85. Cowpox virus but not Vaccinia virus induces secretion of CXCL1, IL-8 and IL-6 and chemotaxis of monocytes in vitro. PMID: 23207068
  86. Data indicate that in NIH3T3 cells expressing unsulfated vGPCR mutant (yydd-vGN) but not vGPCR (wt-vGN) could significantly inhibit the tumor growth triggered by hGRO-alpha. PMID: 23269549
  87. Chemokine CXCL1 dimer is a potent agonist for the CXCR2 receptor. PMID: 23479735
  88. Upregulation of IL-6, IL-8 and CXCL-1 production in dermal fibroblasts by normal/malignant epithelial cells in vitro: Immunohistochemical and transcriptomic analyses. PMID: 23043537
  89. Elevated levels of CXCL1 and CXCL10 could possibly be used as a marker of inflammation and angiogenesis/angiostasis in type 2 diabetes PMID: 23505918
  90. CXCL1 was elevated in acute lymphoblastic leukemia and decreased after bone marrow transplantation. PMID: 22975476
  91. CXCL1/GROalpha increases cell migration and invasion of prostate cancer by decreasing fibulin-1 expression through NF-kappaB/HDAC1 epigenetic regulation. PMID: 23027620
  92. This study demonistrated that CXCL1, CXCL2, CXCL3, CXCL8, and CXCL11, absent from normal muscle fibers, were induced in DMD myofibers. PMID: 23225384
  93. Suppression of CXCL1 and CXCL2 by siRNA or by progesterone and calcitriol inhibits endometrial and ovarian tumor cell invasiveness. PMID: 22615136
  94. IL-17A produced by neutrophils stimulates Gro-alpha secretion from endometrial stromal cells, thereby recruiting more neutrophils and inducing perpetuating inflammation in endometriosis. PMID: 22902060
  95. Seminal plasma rapidly enhanced the expression of the angiogenic chemokines, interleukin (IL)-8 and growth regulated oncogene alpha (GRO) in HeLa cells. PMID: 22732298
  96. Study uncovered a paracrine network, with the chemokines CXCL1 and 2 at its core, that mediates lung metastasis and chemoresistance in breast cancer. PMID: 22770218
  97. CXCL1 rs4074 A allele as a genetic risk factor for cirrhosis progression in hepatitis C. PMID: 22173151
  98. Down-regulation of CXCL1 resulted in a nearly complete prevention of tumor growth. PMID: 22129625
  99. Serum CCL18 and CXCL1 are potentially useful as novel circulating tumor markers for the differential diagnosis between ovarian cancer and benign ovarian masses. PMID: 21928244
  100. Vitamin D3 oppositely regulated the expression of Growth-related oncogene-alpha in THP-1 cells in vitro and human primary monocytes ex vivo via different intracellular signal pathways. PMID: 22251138

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Subcellular Location Secreted
Protein Families Intercrine alpha (chemokine CxC) family
Database Links

HGNC: 4602

OMIM: 155730

KEGG: hsa:2919

STRING: 9606.ENSP00000379110

UniGene: Hs.708652

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