Mouse thyroxine,T4 ELISA Kit

Code CSB-E05083m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


The Mouse thyroxine (T4) ELISA Kit is designed for the quantitative determination of endogenic mouse T4 concentrations in serum, plasma, or tissue homogenates. This assay employs the competitive inhibition enzyme immunoassay technique. Standards or samples are added to the appropriate anti-mouse T4 antibody-pre-coated microtiter plate wells with a Biotin-conjugated T4 antibody. The competitive inhibition reaction is launched between Biotin-labeled T4 and T4 in the sample or standard with the immobilized T4 antibody. Substrate A and B solution are mixed and then added to the wells, and the color turns blue. The color development is terminated after the addition of a stop solution, and the intensity of the color is opposite to the amount of T4 in the sample. Read the OD value with the Microplate reader and calculated the T4 concentration referring to the standard curve. This kit has been quality tested by multiple criteria, including sensitivity, specificity, precision, linearity, and lot-to-lot consistency. Click the product instructions for more information.

Thyroxine/T4 is a hormone secreted into the blood by the thyroid gland. It exerts multiple effects throughout the body, such as the regulation of the heart and digestive function and involvement of metabolism, brain development, bone health, and muscle control. So proper thyroxine levels are vital for health. T4 is converted into T3 after the deiodination. T3-T4 imbalance leads to life-threatening conditions such as myxedema coma and thyroid storm.

Target Name thyroxine,T4
Abbreviation T4
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 20 ng/mL-320 ng/mL
Sensitivity 20 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive


Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.


Typical Data


These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


Materials provided
  • A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-mouse T4 antibody.
  • Standard 6 x 1 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
  • Biotin-conjugated T4 1 x 6 ml
  • HRP-avidin 1 x 6 ml -- HRP catalyzes the TMB to elicit a chromogenic reaction.
  • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
  • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
  • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
  • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm to 630 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Please let us know delivery time and the sulfuric acid content of [stop solution] .

Thanks for your inquiry. The sulfuric acid content of [stop solution] is 4N. Delivery time is usually around 3-5 business days.

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