Rat p53/tumor protein,p53/TP53 ELISA Kit

Instructions
Code CSB-E08336r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name tumor protein p53
Abbreviation TP53
Uniprot No. P10361
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 12.5 pg/mL-800 pg/mL
Sensitivity 3.12 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat p53/TP53 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %100
Range %95-108
1:2Average %87
Range %82-91
1:4Average %90
Range %85-94
1:8Average %92
Range %88-96
Recovery
The recovery of rat p53/TP53 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8984-93
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1OD2AverageCorrected
8002.479 2.615 2.547 2.405
4002.009 1.974 1.992 1.850
2001.356 1.330 1.343 1.201
1000.874 0.929 0.902 0.760
500.532 0.571 0.552 0.410
250.401 0.425 0.413 0.271
12.50.297 0.287 0.292 0.150
00.140 0.143 0.142
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

How can I convert pyggrams per decilitre to picograms per milligram, because your kit is in deciliters and concentrates
picogeram/dl---picogeram/mg.
This kit is according to picogerm/dl, i want according to picogeram /mg.

A:
Thanks for your inquiry. For this kit,convertions are not suggested.We recommend you calculate according to the unit stated in the manual.

Target Data

Function Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.
Involvement in disease p53 is found in increased amounts in a wide variety of transformed cells. p53 is frequently mutated or inactivated in many types of cancer.
Subcellular Location Cytoplasm, Nucleus, Nucleus, PML body, Endoplasmic reticulum, Mitochondrion matrix
Protein Families P53 family
Database Links

KEGG: rno:24842

STRING: 10116.ENSRNOP00000047840

UniGene: Rn.54443

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