Product Details

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 20 pg/ml, corresponding to a specific activity of >5.0x10⁷ IU/mg.

Target Names

IL1B

Uniprot No.

Q28386

Research Area

Immunology

Alternative Names

IL1BInterleukin-1 beta; IL-1 beta

Species

Equus caballus (Horse)

Source

E.coli

Expression Region

116-268aa

Complete Sequence

AAMHSVNCRL RDIYHKSLVL SGACELQAVH LNGENTNQQV VFCMSFVQGE EETDKIPVAL GLKEKNLYLS CGMKDGKPTL QLETVDPNTY PKRKMEKRFV FNKMEIKGNV EFESAMYPNW YISTSQAEKS PVFLGNTRGG RDITDFIMEI TSA

Mol. Weight

17.3 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4, 0.1 % Tween-80

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

IL-1β is a master pro-inflammatory cytokine that drives NF-κB and MAPK signaling cascades, and this recombinant equine form delivers exceptional potency with an ED50 below 20 pg/ml in a D10S cell proliferation assay, corresponding to a specific activity exceeding 5.0 × 10⁷ IU/mg. The tag-free, mature-sequence protein (residues 116–268) produced in *E. coli* faithfully represents the native processed cytokine, making it a suitable basis for equine-specific immune cell activation studies, NF-κB signaling pathway dissection, and use as a reference standard in species-matched cytokine detection assays. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that would otherwise confound innate immune readouts, which supports use in sensitive functional assays such as primary cell proliferation, macrophage activation, and antibody validation by ELISA. Purity exceeding 95% by SDS-PAGE, combined with this stringent endotoxin control, satisfies the quality criteria typical for both *in vitro* mechanistic studies and *in vivo* equine inflammation models.

Target Background

Function

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.

Gene References into Functions

Increased PGE2 production led to reduction in 5-LO products in LPS-treated equine whole blood via IL-1b. PMID: 24530239
Results suggested that chemokine expression by cultured equine BECs following exposure to pulmonary hemorrhage conditions may contribute to the development of inflammatory airway disease in horses. PMID: 22280393
IL-1beta-induced up-regulation of matrix metalloproteinase 13 mRNA was blocked by all concentrations of geldanamycin tested PMID: 17599753
This study examined effects of in vitro exposure to solutions of hay dust, lipopolysaccharides, or beta-glucan on cytokine expression in pulmonary mononuclear cells isolated from healthy horses and horses with recurrent airway obstruction. PMID: 18052742
The effects of semen extender and seminal plasma on the expression of inflammatory modulators in the endometrium of mares are reported. PMID: 18584861
The acute pulmonary neutrophilia characteristic of recurrent airway obstruction was not associated with an increase in expression of chemokines in pulmonary mononuclear cells from disease-susceptible horses. PMID: 19795943

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Subcellular Location

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.

Protein Families

IL-1 family

Database Links

KEGG: ecb:100034237

STRING: 9796.ENSECAP00000000159

UniGene: Eca.13475

Code	CSB-AP003481HO
Abbreviation	Recombinant Horse IL1B protein (Active)
MSDS	MSDS Datasheet
Size	$354
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Recombinant Horse Interleukin-1 beta protein (IL1B) (Active)

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