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| Code | CSB-MP004888HU |
| Size | US$3328 |
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| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
To make this Recombinant Human CD19 protein, the CD19 gene was isolated at first and cloned into an expression vector. CUSABIO has built a mature recombinant protein platform. This Recombinant Human CD19 protein was developed in the platform. It was expressed in Mammalian cell at the region of 20-291aa of the Human CD19 protein. N-terminal 10xHis tag and C-terminal Myc tag was fused with the expression vector for affinity and purification purposes. The purity is 85%+ determined by SDS-PAGE. CD19 is a 95-kDa B-lineage specific transmembrane glycoprotein, which functions as a central response regulator in B lymphocytes by decreasing the threshold for antigen，receptor dependent stimulation, thereby enabling B cell activation when few receptors are engaged. With the important exceptions of hematopoietic stem cells and plasma cells, CD19 is expressed during all stages of B-cell differentiation and is maintained on cells that have undergone neoplastic transformation, being expressed on more than 95% of B-cell non-Hodgkin lymphoma and chronic lymphocytic leukemia. Recent studies have also shown that CD19 expression is maintained despite loss of CD20 expression following treatment with CD20 antibodies, which are frequent components of regimens currently used in the management of these disorders. This strict lineage restriction makes CD19 an attractive immunotherapeutic target and strategies directed at this antigen have become the paradigm for therapies employing chimeric antigen receptors. |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Target Names | CD19 |
| Uniprot No. | P15391 |
| Research Area | Immunology |
| Alternative Names | CD19; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD antigen CD19 |
| Species | Homo sapiens (Human) |
| Source | Mammalian cell |
| Expression Region | 20-291aa |
| Target Protein Sequence | PEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWK Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 35.1 |
| Protein Length | Partial |
| Tag Info |
N-terminal 10xHis-tagged and C-terminal Myc-tagged |
| Form |
Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge.
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| Gene References into Functions |
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| Involvement in disease | Immunodeficiency, common variable, 3 (CVID3) |
| Subcellular Location | Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein. |
| Tissue Specificity | Detected on marginal zone and germinal center B cells in lymph nodes. Detected on blood B cells (at protein level). |
| Database Links |
HGNC: 1633 OMIM: 107265 KEGG: hsa:930 STRING: 9606.ENSP00000437940 UniGene: Hs.652262 |
