Recombinant Rat Carbonic anhydrase 1 (Ca1) gets expressed in E. coli and covers the full length of the mature protein from amino acids 2 to 261. The product comes with an N-terminal 6xHis-SUMO tag that makes purification and detection more straightforward. SDS-PAGE analysis shows a purity level greater than 90%. Research applications requiring precise protein characterization appear to be the main target for this product.
Carbonic anhydrase 1 (Ca1) catalyzes the reversible hydration of carbon dioxide to bicarbonate and protons. The enzyme seems to play a crucial role in regulating pH and ion balance in tissues. Various physiological processes likely depend on its function. Biochemical researchers find Ca1's activity and regulatory mechanisms particularly interesting since they may provide insights into metabolic pathways and potential therapeutic targets.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Carbonic Anhydrase Enzyme Kinetics and Inhibitor Screening
Scientists can use this recombinant rat CA1 protein to set up in vitro enzyme assays for studying carbonic anhydrase catalytic properties and kinetic parameters. The full-length mature protein (2-261aa) expressed in E. coli offers a cost-effective source for high-throughput screening of potential carbonic anhydrase inhibitors. That N-terminal His-SUMO tag makes purification easier and helps with immobilization for automated screening platforms. Researchers can evaluate inhibitor specificity, IC50 values, and structure-activity relationships using this purified enzyme preparation.
2. Antibody Development and Validation
The high purity recombinant rat CA1 protein works well as an immunogen for generating species-specific antibodies against rat carbonic anhydrase 1. Pull-down assays and ELISA-based screening can take advantage of the His-SUMO tag to identify and characterize antibody candidates during hybridoma development. This protein may also serve as a positive control and standard for validating antibody specificity in Western blotting, immunoprecipitation, and other immunoassays targeting rat CA1.
3. Protein-Protein Interaction Studies
Biochemical assays can make use of the tagged recombinant protein to identify and characterize potential binding partners of rat carbonic anhydrase 1. The His tag allows immobilization on nickel-affinity matrices for pull-down experiments with rat tissue lysates or cell extracts. Co-immunoprecipitation studies and surface plasmon resonance analyses can determine binding kinetics and affinities of identified interaction partners, though results might vary depending on experimental conditions.
4. Comparative Species Analysis and Evolutionary Studies
This rat CA1 protein appears to be a valuable tool for comparative biochemistry studies examining carbonic anhydrase function across different mammalian species. Researchers can perform side-by-side enzymatic comparisons with human or mouse carbonic anhydrase orthologs to understand species-specific differences in catalytic efficiency and substrate preferences. The recombinant protein creates controlled experimental conditions for studying evolutionary adaptations in carbonic anhydrase function among rodent species, though some caution is needed when extrapolating results across distantly related species.
